Isolation and Genetic Identification of Dibenzothiophene Degrading Bacteria from Contaminated Soil

Abstract

The biodegradation abilities of 10 dibenzothiophene degrading microbial consortia isolated from contaminated soil were investigated. 5 highly efficient dibenzothiophene degrading bacterial strains were obtained from the consortium LKY10 by screening on LB-agar plates.The bacterial strain LKY10-5 reduced more than 90% of dibenzothiophene with 40 mg•L-1concentration, and had higher degradation efficiency than enriched bacterial consortia in 7 days of cultivation. According to species identification and phylogenetic analysis, strain LKY10-1 and LKY10-3 belonged to Actinobacteria and could be included in Rhodococcus and Cellulosimicrobium genus, LKY10-5 and LKY10-6 belonged to Proteobacteria and could be included in Pseudomonas and Devosia genus, and LKY10-13 could be included in Lysinibacillus genus and belonged to Firmicutes.

Bioaugmentation and biostimulation as strategies for the bioremediation of a burned woodland soil contaminated by toxic hydrocarbons: A comparative study

“…It deserves mentioning that a strain of O. intermedium, formerly isolated from hydrocarbon-rich wastewater and involved in the degradation of HMW hydrocarbons, has recently been described (Gargouri et al, 2011). Besides, a strain of C. funkei able to degrade dibenzothiophene has also been isolated from a hydrocarbon-polluted site (Li et al, 2013) while a C. cellulans strain with a role in oil degradation has been identified in the phyllosphere of Pisum sativum (Al-Awadhi et al, 2012). Furthermore, a strain of L. rhizovicinus was identified in petroleumcontaminated sites (Mahjoubi et al, 2013) as well as in forest soils where it resulted able to degrade chlorophenol (C aliz et al, 2011).…”

Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth

“…Devosia strains are involved in the degradation of the plant toxin deoxynivalenol [72,73], while one study showed bacterial strains identified as potentially Devosia sp. with the ability to degrade dibenzothiophene, an organosulphur compound found in petroleum [74]. However, while it is not possible to say whether Devosia is in some way concerned with plastic metabolization based on these findings alone, these results do merit further study, especially given the interest in discovering potential plastic degraders.…”

The Study of Degradation Characteristics of Isolated <i>Pseudomonas</i> Sp. LKY-5 on Dibenzothiophene

“…As much as 70% of the sulfur in the PASHs may be in the form of benzothiophene, dibenzothiophene (DBT), and more complex thiophenes. DBT is widely accepted as the model compound for biodesulfurization or biodegradation [2][3][4] . So far, biodesulfurization has been studied from 1970s and many efficient desulfurizing bacteria through the 4S pathway were successfully isolated and characterized [5] .…”

“…In this research, the Pseudomonas sp. LKY-5 which had excellent ability of removing DBT was already isolated from oil contaminated soil samples in previous work [3] . It has been reported that the environment factors was influential to the microbial activities in bioremediation [6,7] .…”