Isolation and genetic characterization of <i>Neospora caninum</i> from asymptomatic calves in Spain

Regidor‐Cerrillo, Javier; Gómez-Bautista, Mercedes; Pereira-Bueno, J.; Aduriz, G.; Navarro-Lozano, Vanesa; Risco-Castillo, Verónica; Fernández-García, Aurora; Pedraza-Dı́az, Susana; Ortega‐Mora, Luis Miguel

doi:10.1017/s003118200800509x

Cited by 67 publications

(74 citation statements)

References 21 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, significant biological and genetic differences between isolates of N. caninum are previously reported (Atkinson et al 1999;Schock et al 2001). Nine isolates of N. caninum were made from asymptomatic calves in Spain (Regidor-Cerrillo et al 2008), and one of them (Nc-Spain-1H) failed to induce clinical signs in a BALB/c mouse, grew slowly in vitro and provided protection against fetal death in a pregnant mouse model (Rojo-Montejo et al 2009). …”

Section: Discussionmentioning

confidence: 99%

Comparison of the pathogensis of two isolates of Besnoitia caprae in inbred BALB/c mice

et al. 2010

View full text Add to dashboard Cite

This study was performed to evaluate the infectivity of bradyzoites of two Besnoitia caprae isolates, BC-1 and BC-2, to inbred BALB/c mice. Each group of inbred BALB/c mice was inoculated intraperitoneally with 1 x 10(3), 1 x 10(4), 1 x 10(5), 5 x 10(5) and 1 x 10(6) of one of the two isolates of B. caprae bradyzoites. The mice were monitored daily for a period of 40 days for survival. After death of each mice, several passages from its peritoneal washing and tissues were analyzed using ribosomal DNA-specific PCR assay. Marked differences in pathogenicity between the isolates were seen. All the inbred BALB/c mice infected with BC-2 survived but all the mice that were administered with 1 x 0(5), 5 x 10(5) and 1 x 10(6) BC-1 bradyzoites were died within 4-9 days post-infection (DPI). Histopathological examination of the tissues of the dead mice revealed hyperemia and necrosis with presence of mononuclear and polymorphonuclear cell infiltration in myocardium, spleen and intestines together with interstitial pneumonia and peritonitis. All inbred BALB/c mice in the 1 x 10(3) and 1 x 10(4) groups of BC-1 inoculated mice survived and they were euthanized after 40 DPI. Chronic inflammation with infiltration of mononuclear cells was evident in myocardium, spleen, alveolar septa of the lungs of most of the examined tissues with hemorrhagic enteritis in the mice infected with 1 x 10(6) bradyzoites. The mice infected with different doses of BC-2 were euthanized after 40 DPI and no lesion was seen in histopathological sections of their organs. All peritoneal washings and examined tissues were PCR positive in BC-1 group. This experiment is the first report to show inbred BALB/c mice as a relevant model for B. caprae and demonstrates that this strain of inbred BALB/c mice is a suitable animal model for biological studies and examination of pathogenesis for this species of Besnoitia. The present findings also provide evidence for significant differences between the two isolates of B. caprae.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of the pathogensis of two isolates of Besnoitia caprae in inbred BALB/c mice

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Then, the mouse brains were aseptically removed and a portion was used for in vivo maintenance of the isolate until the cryopreservation of the parasite was carried out, as described . The isolation cultures were maintained with successive passages onto a fresh monolayer of cells every 4-7 days, as outlined by Regidor-Cerrillo et al (2008).…”

mentioning

confidence: 99%

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

García-Melo¹,

Regidor‐Cerrillo

Ortega‐Mora

et al. 2009

Acta Parasitologica

View full text Add to dashboard Cite

Neospora caninum is a tissue-cyst forming parasite that has been recognized worldwide as a cause of abortion in cattle. Despite the ubiquitous distribution of this parasite and its broad range of hosts, the number of N. caninum isolates obtained to date is limited. In addition, the majority of isolates have been obtained from clinically affected hosts, therefore potentially biasing this population towards more virulent isolates. This report describes the isolation and biological characterisation of a new N. caninum isolate, Nc-Goiás 1, obtained from an asymptomatic, naturally infected calf from Brazil. This new isolate was identified as a member of the N. caninum species by polymerase chain reaction (PCR) using specific primers based on the N. caninum internal transcribed spacer 1 (ITS-1) sequence, and was genetically identified at multiple loci using microsatellite analysis. Finally, a pathogenicity study was conducted in a BALB/c mice model. All Nc-Goiás 1-infected mice survived without exhibiting any clinical signs. Further pathogenic characterisation of this isolate suggested that Nc-Goiás 1 is less virulent than other N. caninum isolates (Nc-Liv and Nc-1) studied in this mouse model. This is the first report of the isolation and biological characterisation of N. caninum from an infected but clinically healthy calf in South America.

show abstract

“…It could influence the clinical outcome of the disease and could be directly related with other important epidemiological features, such as the potential for transmission (Regidor-Cerrillo et al 2008, AlQassab et al, 2009. Therefore, it is important to determine genetic diversity of Neospora species, and to link these to pathobiological features and peculiarities.…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Genetic diversity of bovine Neospora caninum determined by microsatellite markers

Salehi

Gottstein

Haddadzadeh

2015

Parasitology International

View full text Add to dashboard Cite

Neospora caninum is one of the most significant parasitic organisms causing bovine abortion worldwide. Despite the economic impact of this infection, relatively little is known about the genetic diversity of this parasite. In this study, using Nc5 and ITS1 nested PCR, N. caninum has been detected in 12 brain samples of aborted fetuses from 298 seropositive dairy cattle collected from four different regions in Tehran, Iran. These specimen (Nc-Iran) were genotyped in multilocus using 9 different microsatellites markers previously described (MS4, MS5, MS6A, MS6B, MS7, MS8, MS10, MS12 and MS21). Microsatellite amplification was completely feasible in 2 samples, semi-completely in 8 samples, and failed in 2 samples. Within the two completely performed allelic profiles of Nc-Iran strains, unique multilocus profiles were obtained for both and novel allelic patterns were found in the MS8 and MS10 microsatellite markers. The Jaccard's similarity index showed significant difference between these two strains and from other standard isolates derived from GenBank such as Nc-Liv, Nc-SweB1, Nc-GER1, A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 KBA1, and KBA2. All samples originating from the same area showed identical allelic numbers and a correlation between the number of repeats and geographic districts was observed.

show abstract

Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain

Cited by 67 publications

References 21 publications

Comparison of the pathogensis of two isolates of Besnoitia caprae in inbred BALB/c mice

Comparison of the pathogensis of two isolates of Besnoitia caprae in inbred BALB/c mice

Isolation and biological characterisation of a new isolate of Neospora caninum from an asymptomatic calf in Brazil

Genetic diversity of bovine Neospora caninum determined by microsatellite markers

Contact Info

Product

Resources

About