Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina

Fontana, Paola; Rago, Alejandro; Fontana, Cecilia Alejandra; Vignolo, Graciela; Cocconcelli, Pier Sandro; Mariotti, J. A.

doi:10.1007/s10658-013-0263-y

Cited by 26 publications

(39 citation statements)

References 22 publications

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“…avenae ( Aaa ), the causal agent of the red stripe disease, results significant yield losses [1,5,6]. For instance, in Argentina the red stripe disease of sugarcane affects 30% of the milling stems and consequently the juice quality [7]. In addition, this disease has similar symptomatology to “false red stripe” caused by a Xanthomonas sp., described firstly in Brazil [8].…”

Section: Introductionmentioning

confidence: 99%

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

et al. 2016

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Sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. Crop productivity is negatively affected by Acidovorax avenae subsp avenae (Aaa), which causes the red stripe disease. Little is known about the molecular mechanisms triggered in response to the infection. We have investigated the molecular mechanism activated in sugarcane using a RNA-seq approach. We have produced a de novo transcriptome assembly (TR7) from sugarcane RNA-seq libraries submitted to drought and infection with Aaa. Together, these libraries present 247 million of raw reads and resulted in 168,767 reference transcripts. Mapping in TR7 of reads obtained from infected libraries, revealed 798 differentially expressed transcripts, of which 723 were annotated, corresponding to 467 genes. GO and KEGG enrichment analysis showed that several metabolic pathways, such as code for proteins response to stress, metabolism of carbohydrates, processes of transcription and translation of proteins, amino acid metabolism and biosynthesis of secondary metabolites were significantly regulated in sugarcane. Differential analysis revealed that genes in the biosynthetic pathways of ET and JA PRRs, oxidative burst genes, NBS-LRR genes, cell wall fortification genes, SAR induced genes and pathogenesis-related genes (PR) were upregulated. In addition, 20 genes were validated by RT-qPCR. Together, these data contribute to a better understanding of the molecular mechanisms triggered by the Aaa in sugarcane and opens the opportunity for the development of molecular markers associated with disease tolerance in breeding programs.

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Section: Introductionmentioning

confidence: 99%

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

et al. 2016

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“…(Wei et al ., ), mottled stripe caused by Herbaspirillum rubrisubalbicans (Saumtally, ), and false red stripe caused by Xanthomonas sp. (Giglioti & Matsuoka, ; Fontana et al ., ). In this study, the occurrence of Aaa, the causal agent of sugarcane red stripe, in five provinces of China was demonstrated using PCR assays and sequencing data.…”

Section: Discussionmentioning

confidence: 99%

“…In preliminary experiments, three previously published primer pairs were tested for detection of A. avenae : Oaf1/Oar1 that targets the 16S–23S rDNA ITS region (Song et al ., ), universal primer pair P0F/P6f (Kihupi et al ., ), and P1b/M1b (Hébert et al ., ) targeting the 16S rDNA region. For unknown reasons, these primers did not work very well in the present investigation for detection of Aaa in sugarcane, despite the fact that primer pair Oaf1/Oar1 had been used for detection of Aaa in rice, oat, maize, millet and sugarcane (Song et al ., ; Fontana et al ., ). The positive amplifications obtained with primers P0F/P6f and P1b/M1b were generally not as strong as with primer pair RS‐ITS‐F1/RS‐ITS‐R1 developed in the current study (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…xyli , and leaf scald caused by Xanthomonas albilineans (Rott et al ., ). Red stripe of sugarcane was first reported in Hawaii in the 1920s, and has since been found in most sugarcane‐growing locations worldwide (Martin & Wismer, ; Rott & Davis, ; Fontana et al ., ). The causal agent of sugarcane red stripe was formerly named Pseudomonas rubrilineans (Martin & Wismer, ) but was later transferred to the genus Acidovorax , the species avenae , and the subspecies avenae of the family Comamonadaceae (Willems et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification and prevalence of Acidovorax avenae subsp. avenae causing red stripe of sugarcane in China

Sun

Rott

et al. 2017

Plant Pathology

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Red stripe caused by the bacterium Acidovorax avenae subsp. avenae (Aaa) is a disease of sugarcane that is distributed worldwide. In this study, 108 sugarcane leaf samples were collected in 2013–2016 from nine sugarcane‐growing regions in China. Aaa was detected by PCR with specific and novel primers from the 16S–23S rDNA internal transcribed spacer region in 81 of 84 (96%) leaves with red stripe symptoms and in 20 of 24 (83%) leaves without symptoms. Furthermore, Aaa was detected in all nine sampling locations representing six sugarcane‐producing provinces in China. The 101 amplified fragments were cloned and sequenced. The size of the nucleotide sequences varied from 436 to 454 bp and the sequence identity ranged from 89.2% to 100%, suggesting a significant genetic variation among Aaa strains from China. Five major restriction fragment length polymorphism (RFLP) profiles were obtained by in silico and polyacrylamide gel electrophoresis analyses of the PCR products digested with HindIII and EcoRI. The causal agent of sugarcane red stripe was also successfully isolated from a diseased plant and its pathogenicity confirmed by inoculation of healthy sugarcane plantlets and reproduction of disease symptoms. The data showed that Aaa is currently widespread in China, suggesting that control methods should be implemented to limit the impact of red stripe on sugarcane production.

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“…In China, sugarcane production is geographically distributed in two regions: Southwest China (Guangxi and Yunnan) and the South China (Fujian and Guangdong), representing one of the main economic resources in the Southwest region. Recently, Guangxi has been the most important region with 60-65% of the total of China sugar production, Sugarcane diseases caused by different pathogens such as fungi, viruses and bacteria [1], may be considered as a major limiting issue affecting crop productivity [2]. Pokkah boeng of sugarcane is an economically important disease worldwide.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Fusarium Verticillioide Isolates from Pokkah Boeng on Sugarcane and The Disease Incidence In Field

Lin

Zhang

Que

et al. 2015

JMEN

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Isolation and genetic characterization of Acidovorax avenae from red stripe infected sugarcane in Northwestern Argentina

Cited by 26 publications

References 22 publications

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

Sugarcane transcriptome analysis in response to infection caused by Acidovorax avenae subsp. avenae

Molecular identification and prevalence of Acidovorax avenae subsp. avenae causing red stripe of sugarcane in China

Characterization of Fusarium Verticillioide Isolates from Pokkah Boeng on Sugarcane and The Disease Incidence In Field

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