Isolation and genetic characteristics of a neurotropic teschovirus variant belonging to genotype 1 in northeast China

Ma, Hailong; Zhang, Mengjia; Ghonaim, Ahmed H.; Su, Fan; He, Qigai

doi:10.1007/s00705-021-04994-3

Cited by 4 publications

(14 citation statements)

References 35 publications

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“…Serum and tissue samples were collected from neonatal pigs with CT-like symptoms on pig farms. All samples were treated as described previously and were negative for PRV, PRRSV, CSFV, and PCV2 (Ma et al, 2021). APPV-positive samples were used for E2 gene sequencing (Supplementary Table S1).…”

Section: Sample Collection Amplification and Sequencingmentioning

confidence: 99%

The Diversity and Spatiotemporally Evolutionary Dynamic of Atypical Porcine Pestivirus in China

Li²,

Zhang³

et al. 2022

Front. Microbiol.

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The presence of congenital tremor (CT) type A-II in newborn piglets, caused by atypical porcine pestivirus (APPV), has been a focus since 2016. However, the source, evolutionary history, and transmission pattern of APPV in China remain poorly understood. In this study, we undertook phylogenetic analyses based on available complete E2 gene sequences along with 98 newly sequenced E2 genes between 2016 and 2020 in China within the context of global genetic diversity. The phylogenies revealed four distinct lineages of APPV, and interestingly, all lineages could be detected in China with the greatest diversity. Bayesian phylogenetic analyses showed that the E2 gene evolves at a mean rate of 1.22 × 10−3 (8.54 × 10−4-1.60 × 10−3) substitutions/site/year. The most recent common ancestor for APPVs is dated to 1886 (1837–1924) CE, somewhat earlier than the documented emergence of CT (1922 CE). Our phylogeographic analyses suggested that the APPV population possibly originated in the Netherlands, a country with developed livestock husbandry, and was introduced into China during the period 1837–2010. Guangdong, as a primary seeding population together with Central and Southwest China as epidemic linkers, was responsible for the dispersal of APPVs in China. The transmission pattern of “China lineages” (lineage 3 and lineage 4) presented a “south to north” movement tendency, which was likely associated with the implementation of strict environmental policy in China since 2000. Reconstruction of demographic history showed that APPV population size experienced multiple changes, which correlated well with the dynamic of the number of pigs in the past decades in China. Besides, positively selected pressure and geography-driven adaptation were supposed to be key factors for the diversification of APPV lineages. Our findings provide comprehensive insights into the diversity and spatiotemporal dynamic of APPV in China.

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Section: Sample Collection Amplification and Sequencingmentioning

confidence: 99%

The Diversity and Spatiotemporally Evolutionary Dynamic of Atypical Porcine Pestivirus in China

Li²,

Zhang³

et al. 2022

Front. Microbiol.

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“…MA-104 cells grown in 96-well plates were used to determine the TCID 50 of the isolated group A CRV with a modification of the method of Reed and Muench [21,30]. The modified protocol was as follows.…”

Section: Viral Isolation and Purificationmentioning

confidence: 99%

“…of 0. 1 [29,30]. After 24 h, when CPE was observed in the MA-104 cells, the MEM was discarded and the cells were washed with PBS.…”

Section: Immunofluorescence Assay (Ifa) Analysismentioning

confidence: 99%

Molecular evolution and genetic characteristics of G3P[3] group A canine rotavirus isolated in Wuhan, China

Chen

et al. 2022

Journal of General Virology

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Rotaviruses can infect multiple animal species and have the potential for cross-recombination based on the segmented genome characteristics. To study the intra-host recombination and zoonotic potential of group A canine rotavirus (CRV), 438 samples were collected from domestic dogs in six animal hospitals and from stray dogs from October 2019 to May 2021 in Wuhan, China. Seven of the samples were positive (7/438) for group A CRV from which a CRV strain was successfully isolated in MA-104 cells. The genotype of the isolated strain was characterized by whole-genome sequencing showing that the genotype was group A CRV G3P[3]. According to the Rotavirus Classification Working Group (RCWG), the genomic constellation of the isolated CRV was G3-P[3]-I3-R3-C3-M3-A9-N2-T3-E3-H6, which belongs to the AU-1-like group with gene segments of AU-1-like and Cat 97-like strains. Based on the phylogenetic analysis of the 11 gene segments, we found that the different segments of the isolated group A CRV were closely related to several reassortment rotaviruses from different animal sources and bat strains. Based on the analysis of the molecular evolution and genetic characteristics, we concluded that the isolated strain might be a reassortment strain. These data further enrich our understanding of rotavirus molecular evolution and genetic characteristics in China.

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“…PTV is globally endemic and circulates in most pig herds. It has been frequently detected in the feces of health pigs suggesting that these viruses could colonize in the intestinal tract without causing any disease [ 1 , 2 , 10 , 11 ]. However, highly pathogenic PTV strains are capable of causing severe clinical signs, including severe to moderate neurological symptoms, reproductive disorders, respiratory diseases, and diarrhea [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…In China, PTV associated disease in pigs was firstly reported in 2003 and a PTV-1 strain (Swine/CH/IMH/03) was isolated in this case [ 1 ]. Since then, a number of PTV strains have been sporadically discovered in multiple provinces in China, mainly associating with asymptomatic infection or milder forms of disease resulting in no more than 10% mortality rate [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel porcine Teschovirus 2 strain as causative agent of encephalomyelitis in suckling piglets with high mortality in China

Liang

Ding

et al. 2023

BMC Vet Res

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Background Porcine Teschovirus (PTV), also named Teschovirus A, is prevalent in pig populations, mainly causing neurological symptoms, diarrhea, pneumonia, and reproductive failure, however the morbidity and mortality are usually low in pig farms. Case presentation In this study, we reported a PTV outbreak investigation in one large-scale pig farm in China with severe symptoms including diarrhea, lethargy, locomotor ataxia, nystagmus, paralysis of the hind limbs, and coma in piglets. More importantly, the mortality reached 38% in suckling pigs, which is remarkably high in PTV history. A novel PTV strain, named HeNZ1, was isolated from cerebral samples of one suckling pig and the genome sequence was obtained by NGS sequencing. Phylogenetic and evolutionary divergence analyses revealed that HeNZ1 belongs to PTV genotype 2. Surprisingly, the VP1 coding region of HeNZ1 shares the highest sequence similarity with European PTV-2 strains, instead of China domestic PTV-2 strains, implying it may not derive from China local PTV-2 strains. Multiple sequence alignment and B cell epitope prediction of PTV VP1 and VP2 protein revealed 10 B cell epitopes, 5 mutant clusters and 36 unique mutation sites, of which 19 unique mutation sites are located in B cell epitopes and exposed on the surface of VP1 or VP2, implying significant antigenic drift potential of HeNZ1. Conclusion These results indicate that HeNZ1 is a highly virulent PTV-2 strain, which capable of causing severe neurological symptoms and high mortality in piglets. Bioinformatic analysis suggest that HeNZ1 is genetically and antigenically different from other Chinese PTV-2 strains. Overall, current case expanded our understanding of PTV-2 clinical spectrum and revealed the emergence of a highly virulent PTV-2 strain with substantial genetic diversity and antigenic drift potential in VP1 and VP2.

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Isolation and genetic characteristics of a neurotropic teschovirus variant belonging to genotype 1 in northeast China

Cited by 4 publications

References 35 publications

The Diversity and Spatiotemporally Evolutionary Dynamic of Atypical Porcine Pestivirus in China

The Diversity and Spatiotemporally Evolutionary Dynamic of Atypical Porcine Pestivirus in China

Molecular evolution and genetic characteristics of G3P[3] group A canine rotavirus isolated in Wuhan, China

Identification of a novel porcine Teschovirus 2 strain as causative agent of encephalomyelitis in suckling piglets with high mortality in China

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