Isolation and functional validation of stress tolerant EaMYB18 gene and its comparative physio-biochemical analysis with transgenic tobacco plants overexpressing SoMYB18 and SsMYB18

Abstract

MYB transcription factors are one of the most important mediators for the survival of plants under multiple stress responses. In the present study, EaMYB18, encoding a single R3 repeat MYB DNA binding domain was isolated from stress-tolerant wild relative species of sugarcane Erianthus arundinaceus. In silico analysis of 948 bp coding mRNA sequence of EaMYB18 exhibited the presence of four exons and three introns. Further, the EaMYB18 gene was transformed in tobacco and its stable inheritance was confirmed through antibiotic resistance screening, PCR amplification and Southern hybridization blotting. Results of the estimation of MDA, proline, total chlorophyll and antioxidant activities of EaMYB18 transgenic tobacco lines exhibited least oxidative damage under drought and cold stress over the untransformed ones, the over-expression of EaMYB18 has improved drought and cold stress tolerance ability in tobacco. The comparative physiological and biochemical analysis of transgenic tobacco plants overexpressing SoMYB18, SsMYB18 and EaMYB18, revealed that the EaMYB18 and SsMYB18 transgenic plants demonstrated effective tolerance to drought and cold stresses, while SoMYB18 showed improved tolerance to salt stress alone. Amongst these three genes, EaMYB18 displayed the highest potential for drought and cold stress tolerances as compared to SoMYB18 and SsMYB18 genes.

Production of biologically active peptides by hydrolysis of whey protein isolates using hydrodynamic cavitation

“…There was a marginal increase in the biochemical parameters with an increase in the concentration of WPI hydrolysate in the sugarcane growth medium, but significantly superior results were observed at 150 and 200 mg/L supplemented medium. In the plant system, the estimation of pigment content is an essential factor for determining the growth [35] , [36] . The pigments have dual functions viz.…”

Meta-analysis of the effect of expression of MYB transcription factor genes on abiotic stress

“…However, the small sample size rendered the results inconclusive. There are many similar physiological indicators related to resistance, such as antioxidant enzyme activity ( Li et al, 2019 ; Li et al, 2020 ; Shingote et al, 2020 ), proline and MDA content ( Gao et al, 2017 ; Shingote et al, 2020 ; Zhang et al, 2012 ) and so on, which are affected by overexpression MYB genes under low temperature stress. However, due to the insufficient sample size of the study, it was not included in the meta-analysis.…”

RNA-Seq-Based Transcriptome Profiling of Early Fruit Development in Chieh-qua and Analysis of Related Transcription Factors

“…7c,7d). EaMYB18 and SsMYB18 transgenic plants showed effective tolerance to drought and cold stress [14]. During the fruit development of chieh-qua, the expression of MYB18 homologous gene in the fruit after pollination for one week was signi cantly higher than that in the other two stages, which also indicated that the stronger stress resistance of old chieh-qua fruits might be related to the expression regulation of transcription factors.…”