Isolation and functional expression of the human atrial natriuretic peptide clearance receptor cDNA

Porter, J G; Arfsten, Ann; Fuller, Forrest; Miller, Judith; Gregory, Lisa C.; Lewicki, John

doi:10.1016/0006-291x(90)91216-f

Cited by 107 publications

(45 citation statements)

References 21 publications

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“…This relationship has since been recognized in the fetal, neonatal, and mature mammalian heart (4,5). Results of our study show a similar relationship in the stage 24 embryonic chick heart.…”

Section: Cycle Length (Ms)supporting

confidence: 84%

“…One receptor, R,, is coupled to guanylate cyclase and is proposed to mediate the known physiologic actions of ANF on target tissues through the production of cyclic GMP (4). A second ANF receptor, R1, is not coupled to guanylate cyclase and is characterized by its ability to bind to various truncated and ring-deleted analogs of ANF (5,6). Although the ANF-RZ receptors have been postulated to serve an ANF storage/clearance function (7), recent data indicate that ANF binding to these receptors stimulates phosphoinositol hydrolysis (8) and inhibits adenylate cyclase and CAMP production (9).…”

Section: Pcan-anf Plasma Clearance Ratementioning

confidence: 99%

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The Effect of Cardiac Cycle Length on Ventricular End-Diastolic Pressure and Maximum Time Derivative of Pressure in the Stage 24 Chick Embryo

Cuneo

et al. 1991

Pediatr Res

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ABSTRACT. We hypothesized that developmental in-(1) have shown that increases in both ventricular EDP and creases in both ventricular end-diastolic pressure (EDP) maximum dP/dt occur concurrently with CL decrease (heart and the maximum time derivative of pressure (dP/dt) ob-rate increase) from d 2 to 6 (Hamburger-Hamilton stages 12 to served in stage 12 to 29 chick embryos are the result of 29) of a 21-d incubation. observed cardiac cycle length (CL) decrease (heart rateWe wondered to what extent the developmental changes in increase). To test this hypothesis, we evaluated EDP and EDP and dP/dt might reflect observed changes in CL. The dP/dt changes that occur during acute CL alterations in purpose of this study, therefore, is to examine whether the the Hamburger-Hamilton stage 24 chick embryo (n = 18). observed increases in both EDP and dP/dt are a function of the Ventricular pressure measurements were obtained with a observed decrease in CL. We evaluated EDP and maximum dP/ servo-null pressure system and digitally recorded at 500 dt during acute CL alterations in the stage 24 (d 4) chick embryo. samples/s. A 1-mm steel probe, heated (decrease CL) or By studying these relationships in a single stage of development, cooled (increase CL), was applied to the sinus venosus. the effect of CL alteration can be assessed independently of other The average baseline CL was 454 ms. The heart rate effects secondary to changes in cardiac morphology. perturbation resulted in CL that varied over a range of 200-2966 ms, assimilating the range of CL change observed during development. Changes in EDP ranged from 0.014 to 0.130 kPa (baseline = 0.061 kPa) and maximum MATERIALS AND METHODS dP/dt ranged from 0.33 to 13.33 kPa/s (baseline = 5.99) Fertile White Leghorn chicken eggs were incubated to HamkPa/s). In each study, EDP varied directly with CL (R2 = burger-Hamilton stage 24 (4 d) at 38°C (2). An egg was removed 0.70). Conversely, maximum dP/dt changes were inversely from the incubator and placed in a heated sand bath (Bioengirelated to CL alterations (R2 = 0.54). Thus, we found that neering Department, Medical College of Georgia) under a radiant there is a direct relationship between changes in CL and warmer to maintain a constant egg temperature of 37-38°C EDP in the stage 24 chick embryo, whereas CL and dP/dt throughout the experiment. The embryo was exposed by removvary inversely. During cardiac development, observed in-ing a portion of the shell and the overlying membranes. The CL creases in maximum dP/dt may be attributed to CL de-was altered using a 1-mm stainless steel probe that was first creases. In contrast, developmental increases in EDP can-heated or cooled in a water bath. The probe was then placed on not be explained by CL decrease and must be accounted the sinus venosus for 10 s to shorten or lengthen the CL. An for by maturational changes in cardiac function in the chick additional 10 control studies were performed using a probe at embryo. (Pediatr Res 29: 338-341, 1991) 37-38°C to exclude pressure of the pr...

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Section: Cycle Length (Ms)supporting

confidence: 84%

Section: Pcan-anf Plasma Clearance Ratementioning

confidence: 99%

The Effect of Cardiac Cycle Length on Ventricular End-Diastolic Pressure and Maximum Time Derivative of Pressure in the Stage 24 Chick Embryo

Cuneo

et al. 1991

Pediatr Res

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“…1A, mRNA expression of none of the natriuretic peptide receptors was detected in monocytes as had been previously reported (13). In contrast, monocytederived immature DCs clearly expressed mRNA of GC-A but not GC-B or NPR-C. Human placenta cDNA was used for a positive control for natriuretic peptide receptor mRNA (35,36). Flow cytometric analysis using anti-human GC-A mAb, A-397, also revealed that expression of GC-A was induced after differentiation of monocytes into DCs (Fig.…”

Section: Immature Dcs But Not Monocytes Express Gc-amentioning

confidence: 61%

Atrial Natriuretic Peptide Polarizes Human Dendritic Cells Toward a Th2-Promoting Phenotype Through Its Receptor Guanylyl Cyclase-Coupled Receptor A

Morita

Ukyo

Furuya

et al. 2003

The Journal of Immunology

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Atrial natriuretic peptide (ANP) is a cardiovascular hormone secreted mainly by the cardiac atria and regulates the volume-pressure homeostasis. The action of ANP is mediated by its receptor, guanylyl cyclase-coupled receptor A (GC-A). In this study, we explored the possibility that ANP and GC-A may play a role in the dendritic cell (DC)-mediated immune regulation. We first examined the expression of GC-A in human monocyte-derived DCs in comparison with monocytes and found that DCs but not monocytes express GC-A at both the mRNA and protein levels. DCs responded to ANP with an increase in intracellular cGMP in a dose-dependent manner, indicating that GC-A expressed on DCs is functional. Furthermore, treatment of DCs with ANP decreased production of IL-12 and TNF-α and conversely increased that of IL-10 upon stimulation with LPS. In accordance with this change of cytokine production, DCs treated with ANP plus LPS promoted differentiation of naive CD4+ T cells into a Th2 phenotype. Finally, we presented evidence that ANP affected cytokine production of fresh whole blood stimulated with LPS in line with the above-mentioned results. These results indicate that ANP polarizes human DCs toward a Th2-promoting phenotype through GC-A and thus can regulate immune responses.

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“…This was shown by the demonstrations that the ligand binding activities of bovine 1341 and eel [27] NPR-C were not impaired by site-directed mutagenesis of the Cys residues involved in the interchain disulfide bonds. Also, a soluble form of the extracellular domain of human NPR-C, which was produced by truncating at a juxtamembrane site so that it did not contain the membrane-spanning and cytoplasmic domains, nor the Cys residue responsible for the interchain disulfide linkage, retained nearly full binding activity [39]. In the case of the guanylatecyclase-linked receptors, however, the oligomerization appears to be essential for the expression of the cyclase activity [40] in analogy to adenylate cyclase (41, 421 and soluble guanylate cyclase [43].…”

Section: Discussionmentioning

confidence: 99%

Cloning and Properties of a Novel Natriuretic Peptide Receptor, NPR‐D

Kashiwagi

Katafuchi

Kato

et al. 1995

European Journal of Biochemistry

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A novel natriuretic peptide receptor, which we have termed natriuretic peptide receptor D (NPR-D), has been cloned and characterized. cDNAs related to the natriuretic peptide receptor (NPR) were amplified by PCR from a template of poly(A)-rich RNA isolated from the eel gill. Sequencing of the PCR products revealed the presence of a new clone that showed about 70% sequence identity to the eel type-C receptor, NPR-C. The PCR fragment was used to determine the tissue distribution of the new NPR-D message by an RNase protection assay, which gave the strongest signal in brain samples, and then used to screen a brain library to obtain a full-length cDNA clone. The cDNA clone predicted a protein of 500 amino acids containing a signal sequence and a hydrophobic transmembrane segment. The predicted sequence also contained the NPR motif which is essential for the binding of natriuretic peptides. The protein NPR-D was expressed in COS cells and shown to have high affinities for eel and rat natriuretic peptides. The newly cloned NPR-D has a short cytoplasmic tail; in this respect, NPR-C and NPR-D are very similar and form a subfamily of the NPR family. Affinity labeling indicated that NPR-D exists as a disulfide-linked tetramer. This is a marked contrast to the homodimeric structure of NPR-C. HS-142-1, a non-peptide natriuretic peptide receptor antagonist of microbial origin previously shown to be selective for the guanylate-cyclase-coupled receptors NPR-A and NPR-B, competitively inhibited the binding of "'I-labeled eel natriuretic peptide to eel NPR-D, whereas it did not affect the binding activity of eel NPR-C, suggesting that HS-142-1 is an antagonist that recognizes the tetrameric structures of NPR since the guanylate-cyclase-coupled receptors have also been demonstrated to exist as tetramers.Keywords: antagonist; cloning; eel ; natriuretic peptide; receptor.The natriuretic peptide system is involved in the central and peripheral control of body fluid volume and blood pressure [ 1 -61. In mammals, three peptides, natriuretic peptides A, B and C (ANP, BNP and CNP, respectively), have been identified as the members of the natriuretic peptide family and shown to have diverse biological actions including diuresis, natriuresis, vasorelaxation, anti-mitogenesis, and modulation of other regulatory systems. Comparative studies using non-mammalian species revealed the presence of additional members; for example, two types of CNP have been cloned from the frog [ 7 ] , and from the eel ventricle, ventricular natriuretic peptide has been isolated and suggested to be a fourth member of the family [8].

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Isolation and functional expression of the human atrial natriuretic peptide clearance receptor cDNA

Cited by 107 publications

References 21 publications

The Effect of Cardiac Cycle Length on Ventricular End-Diastolic Pressure and Maximum Time Derivative of Pressure in the Stage 24 Chick Embryo

The Effect of Cardiac Cycle Length on Ventricular End-Diastolic Pressure and Maximum Time Derivative of Pressure in the Stage 24 Chick Embryo

Atrial Natriuretic Peptide Polarizes Human Dendritic Cells Toward a Th2-Promoting Phenotype Through Its Receptor Guanylyl Cyclase-Coupled Receptor A

Cloning and Properties of a Novel Natriuretic Peptide Receptor, NPR‐D

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