Isolation and functional characterisation of the PHT1 gene encoding a high-affinity phosphate transporter in<i>Camellia oleifera</i>

Zhou, Jinlin; Lu, Mengqi; Zhang, Chenhui; Qu, Xinjing; Liu, Yiyao; Yang, Jin Ok; Yuan, Jiangang

doi:10.1080/14620316.2019.1703562

Cited by 6 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The multiple sequence alignment of the EaPHT1;2 protein with the reference genomes was carried out by utilizing the Clustal X program, and as illustrated in Figure 3B, the EaPHT1;2 transporter is identical to other PHT1 proteins, which possess the characteristic motif sequence GGDYPLSATIMSE (GGDYPLSATIxSE) [45][46][47]. The secondary structure showed 10 transmembrane domains with a hydrophilic loop in the central part of the domains, 39 phosphorylation sites, and 1 N-glycosylation site [13,24,48,49]. The 3D structure of many plant PHT proteins is still not available for comparative analysis with the phosphate transporter genes; therefore, according to the homology in SWISS-MODEL, a 7sp5.1.A template was chosen to construct the 3D structure with 92.4% sequence coverage, followed by validation, and the binding sites of the EaPHT1;2 protein structure were analyzed.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco

Naveenarani,

Swamy,

Surya Krishna

et al. 2023

Plants

View full text Add to dashboard Cite

Phosphorus deficiency highly interferes with plant growth and development. Plants respond to persistent P deficiency by coordinating the expression of genes involved in the alleviation of stress. Promoters of phosphate transporter genes are a great choice for the development of genetically modified plants with enhanced phosphate uptake abilities, which improve crop yields in phosphate-deficient soils. In our previous study, the sugarcane phosphate transporter PHT1;2 gene showed a significantly high expression under salinity stress. In this study, the Erianthus arundinaceus EaPHT1;2 gene was isolated and characterized using various in silico tools. The deduced 542 amino acid residues have 10 transmembrane domains, with a molecular weight and isoelectric point of 58.9 kDa and 9.80, respectively. They displayed 71–96% similarity with Arabidopsis thaliana, Zea mays, and the Saccharum hybrid. To elucidate the function of the 5′ regulatory region, the 1.1 kb promoter was isolated and validated in tobacco transgenics under Pi stress. The EaPHT1;2 promoter activity was detected using a β-glucuronidase (GUS) assay. The EaPHT1;2 promoter showed 3- to 4.2-fold higher expression than the most widely used CaMV35S promoter. The 5′ deletion analysis with and without 5′ UTRs revealed a small-sized 374 bp fragment with the highest promoter activity among 5′ truncated fragments, which was 2.7 and 4.2 times higher than the well-used CaMV35S promoter under normal and Pi deprivation conditions, respectively. The strong and short promoter of EaPHT1;2 with 374 bp showed significant expression in low-Pi-stress conditions and it could be a valuable source for the development of stress-tolerant transgenic crops.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco

Naveenarani,

Swamy,

Surya Krishna

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Other studies have examined the high-affinity Pi transporter gene and have characterized rbcL and rbcS genes from C. oleifera ( Chen et al, 2015 ; Zhou et al, 2020 ). These findings are useful for identifying promising cultivars ( Figure 2 )…”

Section: Research On the Biology Of C Oleiferamentioning

confidence: 99%

Applications of Chinese Camellia oleifera and its By-Products: A Review

et al. 2022

View full text Add to dashboard Cite

Camellia oleifera is a woody oil tree species unique to China that has been cultivated and used in China for more than 2,300 years. Most biological research on C. oleifera in recent years has focused on the development of new varieties and breeding. Novel genomic information has been generated for C. oleifera, including a high-quality reference genome at the chromosome level. Camellia seeds are used to process high-quality edible oil; they are also often used in medicine, health foods, and daily chemical products and have shown promise for the treatment and prevention of diseases. C. oleifera by-products, such as camellia seed cake, saponin, and fruit shell are widely used in the daily chemical, dyeing, papermaking, chemical fibre, textile, and pesticide industries. C. oleifera shell can also be used to prepare activated carbon electrodes, which have high electrochemical performance when used as the negative electrode of lithium-ion batteries. C. oleifera is an economically valuable plant with diverse uses, and accelerating the utilization of its by-products will greatly enhance its industrial value.

show abstract

“…In Arabidopsis thaliana, the PHT1 family comprises nine phosphorus transporters. AtPHT1-1 and AtPHT1-4 function as high-affinity phosphate transporters under varying phosphorus levels, with their expression being upregulated under conditions of phosphorus deficiency [31] and exhibiting high transcription in the roots [32]. In rice (Oryza sativa), OsPht1, part of the Pht8 family, regulates phosphate homeostasis [33].…”

Section: Introductionmentioning

confidence: 99%

NopC/T/L Signal Crosstalk Gene GmPHT1-4

Zhu,

Yu,

et al. 2023

IJMS

View full text Add to dashboard Cite

Symbiotic nodulation between leguminous plants and rhizobia is a critical biological interaction. The type III secretion system (T3SS) employed by rhizobia manipulates the host’s nodulation signaling, analogous to mechanisms used by certain bacterial pathogens for effector protein delivery into host cells. This investigation explores the interactive signaling among type III effectors HH103ΩNopC, HH103ΩNopT, and HH103ΩNopL from SinoRhizobium fredii HH103. Experimental results revealed that these effectors positively regulate nodule formation. Transcriptomic analysis pinpointed GmPHT1-4 as the key gene facilitating this effector-mediated signaling. Overexpression of GmPHT1-4 enhances nodulation, indicating a dual function in nodulation and phosphorus homeostasis. This research elucidates the intricate regulatory network governing Rhizobium–soybean (Glycine max (L.) Merr) interactions and the complex interplay between type III effectors.

show abstract

Isolation and functional characterisation of the PHT1 gene encoding a high-affinity phosphate transporter inCamellia oleifera

Cited by 6 publications

References 52 publications

Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco

Isolation and Characterization of Erianthus arundinaceus Phosphate Transporter 1 (PHT1) Gene Promoter and 5′ Deletion Analysis of Transcriptional Regulation Regions under Phosphate Stress in Transgenic Tobacco

Applications of Chinese Camellia oleifera and its By-Products: A Review

NopC/T/L Signal Crosstalk Gene GmPHT1-4

Contact Info

Product

Resources

About