Isolation and Functional Analysis of VvWRKY28, a Vitis vinifera WRKY Transcription Factor Gene, with Functions in Tolerance to Cold and Salt Stress in Transgenic Arabidopsis thaliana

Abstract

The grape (Vitis vinifera L.) not only has a long history of cultivation, but also has rich nutritional value and high economic value. However, grapes often face many threats in the growth process. For example, low temperature and salt stress restrict the growth status, yield, and geographical distribution of grapes. WRKY, as one of the largest transcription factor (TF) families in plants, participates in the response of plants to stress. VvWRKY28, a new zinc finger type transcriptional regulator gene, was isolated from Beichun (V. vinifera×V.amurensis) in this study. From the subcellular localization results, it can be concluded that VvWRKY28 was localized in the nucleus. The expression of VvWRKY28 was enriched in leaves (young and mature leaves), and cold and high salt conditions can induce high expression of VvWRKY28. After being transferred into Arabidopsis, VvWRKY28 greatly improved the tolerance of Arabidopsis to low temperature and high salt and also changed many physiological and biochemical indicators of transgenic Arabidopsis to cope with cold and high salt stimulation. The content of malondialdehyde (MDA) was decreased, but for chlorophyll and proline, their content increased, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were improved. In addition, under cold stress, binding with cis-acting elements promotes the expression of downstream genes related to cold stress (RAB18, COR15A, ERD10, PIF4, COR47, and ICS1). Moreover, it also plays an active role in regulating the expression of genes related to salt stress (NCED3, SnRK2.4, CAT2, SOD1, SOS2, and P5CS1) under salt stress. Therefore, these results provide evidence that VvWRKY28may play a role in the process of plant cold and salt stress tolerance.

Brassica napus Transcription Factor Bna.A07.WRKY70 Negatively Regulates Leaf Senescence in Arabidopsis thaliana

“…The WRKY proteins are one of the largest and most important superfamilies of transcription factors (TFs) in plants. WRKY transcription factors encompass a core motif WRKYGQK (a highly conserved WRKY domain) at the N-terminus and an atypical Zinc finger motif at the C-terminus [ 12 ]. On the basis of both the number of WRKY domains and the features of the Zn-finger motif in their evolutionary history, the WRKY TFs can be divided into three different groups (I, II, and III).…”

Identification of Hsp20 gene family in Malus domestica and functional characterization of Hsp20 class I gene MdHsp18.2b

“…Moreover, H 2 O 2 may also act as signalling molecules that activate related signalling pathways to regulate salt stress responses. In addition, Hsp20 might act as a chaperone protein to co‐regulate salt stress resistance together with other proteins, such as transcription factors FvMYB82 (from Fragaria vesca ), MbCBF1 (from Malus baccata ), and VvWRKY28 (from Vitis vinifera ), which are positive regulators of salt tolerance (Liu et al, 2022; Liang et al, 2022; Li et al, 2022).…”

The Role of MaWRKY70 in Regulating Lipoxygenase Gene Transcription during Chilling Injury Development in Banana Fruit

“…CsWRKY46 confers cold tolerance by modulating cold-stress-responsive genes in cucumber [ 37 ]. A cold-induced WRKY gene in grape, VvWRKY28 , regulated the expression of cold-stress-related genes and played a role in cold stress tolerance [ 38 ]. In this study, a CI-inducible WRKY TF, MaWRKY70, was identified.…”