Isolation and expression studies of the ERD15 gene involved in drought-stressed responses

Abstract

ABSTRACT. The early response to the dehydration 15 (ERD15) gene is widely involved in the processes of signal transduction, programmed cell death, gene transcription, and stress tolerance in plants. In a previous study, the ERD15 gene was shown to be an important regulator of the abscisic acid response and salicylic acid-dependent defense pathway, acting as an important negative regulator of abscisic acid. The complete IbERD15 gene (accession No. KF723428) was isolated by reverse transcription-polymerase chain reaction. The IbERD15 gene contains an open reading frame of 504 bp, encodes a peptide of 167 amino acids, and has a molecular mass of 18.725 kDa. The transcript levels of the IbERD15 gene in a variety of tissues were examined by digital gene expression profiling. The roots of the sweet potato were treated by 3 degrees of polyethylene glycol, and the results indicate that the IbERD15 gene might play an important role in the defense response to drought stress. Moreover, the IbERD15 gene was successfully transformed into yeast cells for analysis of drought tolerance in transgenic yeast.

Ocimum metabolomics in response to abiotic stresses: Cold, flood, drought and salinity

“…The sixth transcript “EARLY RESPONSIVE TO DEHYDRATION 15 or ERD15 (CDS_17616_Unigene_29085)” was found to be up-regulated in DGE and qRT-PCR results. Shao et al [65] have reported that ERD15 gene from the roots of sweet potato is involved in defense response to drought which could be related to the fact that Ocimum plant was able to withstand drought until a period of 30 days. “Receptor kinase FERONIA” (CDS_32039_Unigene_49003) is the next transcript taken up for validating the up-regulation in the DGE and qRT-PCR data.…”

Overexpression of BplERD15 Enhances Drought Tolerance in Betula platyphylla Suk.

“…The ERD15 from sweet potato (Ipomoea batatas (L.) Lam. ), IbERD15, has been reported to play an important role in the response to drought stress [39].…”

Research Article Evaluation of extraction methods for obtaining high-quality RNA from sweet potato

“…Hou et al (2021) used the TRIzol method to isolate RNA and analyze genes from the Bgalactosidase family, which are involved in the response of biotic and abiotic stresses. In addition, the TRIzol method has also been used on the transcriptome sequencing of sweet potato (Tao et al, 2012), and on the characterization of ERD15, a gene that seems to be involved in the defense response to water stress in sweet potato (Shao et al, 2014), to identify water deficit responsive genes in RNAseq and RT-qPCR studies (Arisha et al, 2020), in the validation of reference genes for the expression normalization of miRNAs (Liu et al, 2020), and for the evaluation of genes associated to starch and sucrose metabolism (Ma et al, 2020;Qin et al, 2021).…”