Isolation and expression pattern of genes related to flower initiation in the evergreen azalea, Rhododendron×pulchrum ‘Oomurasaki’

Cheon, Kyeong-Seong; Nakatsuka, Akira; Kobayashi, Nobuo

doi:10.1016/j.scienta.2011.09.001

Cited by 21 publications

(18 citation statements)

References 42 publications

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“…Quantitation was performed by using the difference in the threshold cycle values (ΔCt) between the two samples (the target genes and the reference genes, both azalea ACTIN and Histone H3) to calculate the relative amounts of the template present. The value of the relative expression was calculated as the standard using the maximum value for the RoPI gene, and the mean and standard error (SE) were calculated from the value of the relative expression (Cheon et al, 2011).…”

Section: Expression Analysis Of Kurume Azalea Pi/glo Homologmentioning

confidence: 99%

“…In contrast to other plants, floral form mutations in azaleas have been poorly investigated at the molecular level. After initiation of floral bud formation, RpAP3 expression was increased in the floral buds of Rhododendron × pulchrum (Cheon et al, 2011), and RkAG was overexpressed in the second whorl of staminoid-petaled cultivars of R. kaempferi (Tasaki et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Mutant <i>PI/GLO</i> Homolog Confers the Hose-in-hose Flower Phenotype in Kurume Azaleas

2016

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To analyze hose-in-hose flowers of Kurume azaleas, floral morphologies and homeotic MADS genes were investigated in single and hose-in-hose cultivars. In floral morphological analysis, single flowers are comprised of four whorls of floral organs: sepals, petals, stamens, and carpels. Hose-in-hose flowers, in contrast, produced petaloid sepals instead of sepals in the first whorl. Based on molecular analysis, we isolated PISTILLATA (PI)/GLOBOSA (GLO) homologs in the hose-in-hose flower cultivar 'Kirin' and analyzed their expression in single flower and hose-in-hose flower cultivars. The mRNA sequences of two RoPI genes, RoPI-1 and RoPI-2, differed in terms of a 6 bp deletion. Based on genomic analysis, an insertion sequence was found in the second intron of RoPI-1 in hose-in-hose flowers. These RoPI genes were mainly expressed in the second and third whorls of single flowers, whereas these genes were expressed in all whorls of hose-in-hose flowers. These results suggest that the up-regulation of RoPI-1 expression contributed to the petaloid sepal formation of hosein-hose flowers of Kurume azaleas.

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Section: Expression Analysis Of Kurume Azalea Pi/glo Homologmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mutant <i>PI/GLO</i> Homolog Confers the Hose-in-hose Flower Phenotype in Kurume Azaleas

2016

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“…The primer pairs used for the RT-qPCR assay were reported previously (Cheon et al, 2011(Cheon et al, , 2012. In the RT-qPCR assay, 0.5 μl of the cDNA samples was used as the template in a 20 μl RT-qPCR assay mix containing 10 μl SYBR Premix Ex Taq II (TaKaRa Bio) and 0.25 μM of each primer.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

“…Each stage of floral organ formation was collected on 24 July and 7 August. RT-qPCR was performed with gene-specific primers (Cheon et al, 2011(Cheon et al, , 2012. Data are the mean ± SE (n = 3; normalized against both Histone H3 and ACTIN).…”

Section: Observation Of Floral Organ Developmentmentioning

confidence: 99%

“…The expression pattern of these genes and shoot growth were used to determine the putative timing of the initiation of floral bud formation (Cheon et al, 2011(Cheon et al, , 2012; however, the expression pattern of these genes, in relation to morphological changes of the floral meristem in the shoot apex, remains unclear. In this study, the evergreen azalea 'Oomurasaki', which has a single flowering season, was used to examine microscopically the developmental stage of floral organs during floral bud formation, because 'Oomurasaki' has larger shoots and floral buds than other evergreen azaleas.…”

Section: Introductionmentioning

confidence: 99%

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Expression Pattern of Several Flowering-related Genes during Flower Bud Formation in Rhododendron × pulchrum ‘Oomurasaki’

Cheon

Nakatsuka

Tasaki

et al. 2013

J. Japan. Soc. Hort. Sci.

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To investigate the relationship between the development of floral buds and the expression pattern of floweringrelated genes, observation of floral organ development and expression analysis of RpFT, RpSOC1, RpLFY, RpTFL1, RpCEN, and RpAP3 were carried out from July to August 2011 of the evergreen azalea 'Oomurasaki'. After floral meristem development, floral organs, such as sepals, petals, stamens, and carpels, were observed subsequently on the floral meristems. The expression level of RpTFL1 gradually decreased with floral meristem development; however, the expression level of RpFT and RpLFY increased continuously until carpel development and that of RpAP3 began to increase with stamen development. RpSOC1 and RpCEN showed an irregular expression pattern during floral bud development. These results suggest that the period of floral organ development can be determined by the expression pattern of RpTFL1, RpFT, RpLFY, and RpAP3 in evergreen azalea 'Oomurasaki'.

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Characterization of the MIKCC-type MADS-box gene family in blueberry and its possible mechanism for regulating flowering in response to the chilling requirement

Zhang,

Wu,

Zhang

et al. 2024

Planta

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Isolation and expression pattern of genes related to flower initiation in the evergreen azalea, Rhododendron×pulchrum ‘Oomurasaki’

Cited by 21 publications

References 42 publications

Mutant <i>PI/GLO</i> Homolog Confers the Hose-in-hose Flower Phenotype in Kurume Azaleas

Mutant <i>PI/GLO</i> Homolog Confers the Hose-in-hose Flower Phenotype in Kurume Azaleas

Expression Pattern of Several Flowering-related Genes during Flower Bud Formation in Rhododendron × pulchrum ‘Oomurasaki’

Characterization of the MIKCC-type MADS-box gene family in blueberry and its possible mechanism for regulating flowering in response to the chilling requirement

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