Isolation and expression of the homeobox gene <i>Gbx1</i> during mouse development

Rhinn, Muriel; Lun, Klaus; Werner, Michaela; Simeone, Antonio; Brand, Michael

doi:10.1002/dvdy.10435

Cited by 31 publications

(41 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As in other vertebrates, this precise alignment suggests that forming the otx2/gbx1 interface is a crucial step for positioning the MHB in the neural plate. Indeed, Otx and Gbx loss-and gain-offunction experiments in different species support the notion that these genes are required to correctly position the MHB in the neural plate (Wassarman et al, 1997;Rhinn et al, 1998;Rhinn et al, 2004;Acampora et al, 1998;Broccoli et al, 1999;Millet et al, 1999;Li and Joyner, 2001;Martinez-Barbera et al, 2001;Kikuta et al, 2003) (M.R. and M.B., unpublished).…”

Section: Introductionmentioning

confidence: 78%

Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling

et al. 2005

Self Cite

View full text Add to dashboard Cite

The organizing center located at the midbrain-hindbrain boundary (MHB)patterns the midbrain and hindbrain primordia of the neural plate. Studies in several vertebrates showed that the interface between cells expressing Otx and Gbx transcription factors marks the location in the neural plate where the organizer forms, but it is unclear how this location is set up. Using mutant analyses and shield ablation experiments in zebrafish, we find that axial mesendoderm, as a candidate tissue, has only a minor role in positioning the MHB. Instead, the blastoderm margin of the gastrula embryo acts as a source of signal(s) involved in this process. We demonstrate that positioning of the MHB organizer is tightly linked to overall neuroectodermal posteriorization, and specifically depends on Wnt8 signaling emanating from lateral mesendodermal precursors. Wnt8 is required for the initial subdivision of the neuroectoderm,including onset of posterior gbx1 expression and establishment of the posterior border of otx2 expression. Cell transplantation experiments further show that Wnt8 signaling acts directly and non-cell-autonomously. Consistent with these findings, a GFP-Wnt8 fusion protein travels from donor cells through early neural plate tissue. Our findings argue that graded Wnt8 activity mediates overall neuroectodermal posteriorization and thus determines the location of the MHB organizer.

show abstract

Section: Introductionmentioning

confidence: 78%

Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, it has been shown that mice null for Gbx1, which marks a subset of dIL A neurons (John et al, 2005), show no aversive behaviors but do have abnormal hindlimb gait (Buckley et al, 2013;Meziane et al, 2013 preprint). Given that this was a complete Gbx1 knockout, and knowing that Gbx1 is expressed more broadly in the ventricular zone of the caudal neural tube and regions that will develop into the hindbrain and inhibitory cortical interneurons (Buckley et al, 2013;John et al, 2005;Rhinn et al, 2004), the manipulation of Gbx1 lineage neurons specifically in the spinal cord is necessary before a definitive contribution of dIL A neurons to the gait phenotype can be concluded. Furthermore, as analyses of subsets of Ptf1a lineage neurons become more refined, the full extent to which inhibitory neurons gate or attenuate somatosensory inputs will be revealed.…”

Section: Inhibitory Neuronsmentioning

confidence: 99%

Making sense out of spinal cord somatosensory development

2016

View full text Add to dashboard Cite

The spinal cord integrates and relays somatosensory input, leading to complex motor responses. Research over the past couple of decades has identified transcription factor networks that function during development to define and instruct the generation of diverse neuronal populations within the spinal cord. A number of studies have now started to connect these developmentally defined populations with their roles in somatosensory circuits. Here, we review our current understanding of how neuronal diversity in the dorsal spinal cord is generated and we discuss the logic underlying how these neurons form the basis of somatosensory circuits.

show abstract

“…Then, we studied about cooperative interplay of Gbx1, TG2/Gh and TGF-b2 as they are reported to be induced individually by RA in cultures of chick embryonic skin (Obinata et al, 2001), mouse epidermal cells (Lichti and Yuspa 1985a) and human hair follicles (Foitzik et al, 2005) respectively. Studies of Gbx1 have concentrated on the brain or neurons (Rhinn et al, 2004;Asbreuk et al, 2002) and are poor in other tissues. RA induces a catagen-like stage in human hair follicles, accompanying upregulation of TGF-b2 in the dermal papilla, resulting in hair loss (Foitzik et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Tgm2/Gh, Gbx1 and TGF-beta are involved in retinoic acid-induced transdifferentiation from epidermis to mucosal epithelium

Obinata¹,

Osakabe²,

Yamaguchi³

et al. 2011

Int. J. Dev. Biol.

View full text Add to dashboard Cite

�e previousl� demonstrated that retinoic acid �RA� induces epidermis to transdifferen�e previousl� demonstrated that retinoic acid �RA� induces epidermis to transdifferentiate to mucosal epithelium with goblet cells in chick embr�onic cultured skin. To characterize the molecular mechanism of this transdifferentiation process, we used rat embr�onic cultured skin and immunohistochemistr� to confirm that RA-induced epidermal transdifferentiation accompanies the expression of markers of esophagus epithelium. Because Gbx1, TG2/Gh �transglutaminase2� and TGF-b2 are reported individuall� to be induced b� RA in cultures of chick embr�onic skin, mouse epidermal cells and human hair follicles respectivel�, here, we investigated whether cooperative interpla� of Gbx1, TG2/Gh and TGF-b2 is required for the transdifferentiation of epidermal cells to mucosal cells. �e have shown that expression of Gbx1, TG2/Gh and TGF-b proteins were all upregulated in RA-induced transdifferentiated skin and that the former two were expressed in the epidermis, while TGF-b was expressed in the dermis. Inhibitors of the TGF-b signal pathwa� partiall� inhibited transdifferentiation. Overexpression of both hTG2/Gh and mGbx1 together in the epidermis b� electroporation resulted in cuboidal cells in the upper cell la�ers of the epidermis without keratinized la�ers, although epidermal keratinization was observed in skin b� overexpression of either of them. Labeling DNA with BrdU indicated that RA directl� transdifferentiated transient amplif�ing epidermal cells, not stem cells, to mucosal cells. This stud� showed that coexpression of TG/2 and Gbx1 in the epidermis was required for esophagus-like mucosal transdifferentiation, and that increase in TGF-b2 expression b� RA in the dermis was essential to induce transdifferentiation through epithelial-mesench�mal interaction.

show abstract

Isolation and expression of the homeobox gene Gbx1 during mouse development

Cited by 31 publications

References 33 publications

Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling

Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling

Making sense out of spinal cord somatosensory development

Tgm2/Gh, Gbx1 and TGF-beta are involved in retinoic acid-induced transdifferentiation from epidermis to mucosal epithelium

Contact Info

Product

Resources

About