Isolation and expression of HMG-CoA synthase and HMG-CoA reductase genes in different development stages, tissues and treatments of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae)

Yu, Junjian; Dai, Lulu; Zhang, Ranran; Li, Zhumei; Pham, Thanh; Chen, Hui

doi:10.1016/j.cbpb.2015.04.015

Cited by 9 publications

(10 citation statements)

References 38 publications

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“…According to the expression patterns observed in GPPS / FPPS and GGPPS genes, it is unlikely that frontalin or ipsdienol are produced in this species, despite D . rhizophagus males presenting higher expression levels than females, as has been reported in other bark beetle species that produce these pheromones [27,32,48,67]. These findings were also supported by the GC-MS analysis, which did not record the present of these compounds.…”

Section: Discussionsupporting

confidence: 78%

“…With respect to MVA pathway genes in JH III stimulated insects, an irregular expression pattern was displayed, and there was no tendency for a specific sex or time (Figure 2). However, other studies in bark beetles have reported that JH III induced some of these genes, showing high expression levels mainly in males, or at least higher compared to females [25,27,28,48,67,68].…”

Section: Discussionmentioning

confidence: 99%

“…These last genes achieved similar expression levels in D. armandi males both in phloem-fed insects and JH III stimulated insects [48], but in I. confusus , both genes reached higher expression levels in phloem-fed insects than in stimulated insects. In contrast to what was observed in fed insects, neither the enzymatic activity of HMGS, HMGR, and GPPS nor pheromone production was recorded in the JH III stimulated insects of I .…”

Section: Discussionmentioning

confidence: 99%

“…Several in vitro assays have documented that the feeding and topical application of JH III in some bark beetle species—such as Ips confusus , Ips pini [47], Dendroctonus armandi [48] and Dendroctonus jeffreyi [24,49]—increases the transcription levels from the MVA pathway genes, and in some cases, it also induces the production of ipsdienol or frontalin in the midgut tissue [24,25,26]. Because frontalin and ipsdienol were not previously identified in the gut of D .…”

Section: Introductionmentioning

confidence: 99%

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The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

Sarabia

López

Obregón-Molina

et al. 2019

IJMS

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Bark beetles commonly produce de novo terpenoid pheromones using precursors synthesized through the mevalonate pathway. This process is regulated by Juvenile Hormone III (JH III). In this work, the expression levels of mevalonate pathway genes were quantified after phloem feeding—to induce the endogenous synthesis of JH III—and after the topical application of a JH III solution. The mevalonate pathway genes from D. rhizophagus were cloned, molecularly characterized, and their expression levels were quantified. Also, the terpenoid compounds produced in the gut were identified and quantified by Gas Chromatography Mass Spectrometry (GC-MS). The feeding treatment produced an evident upregulation, mainly in acetoacetyl-CoA thiolase (AACT), 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS), 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), phosphomevalonate kinase (PMK), and isopentenyl diphosphate isomerase (IPPI) genes, and males reached higher expression levels compared to females. In contrast, the JH III treatment did not present a clear pattern of upregulation in any sex or time. Notably, the genes responsible for the synthesis of frontalin and ipsdienol precursors (geranyl diphosphate synthase/farnesyl diphosphate synthase (GPPS/FPPS) and geranylgeranyl diphosphate synthase (GGPPS)) were not clearly upregulated, nor were these compounds further identified. Furthermore, trans-verbenol and myrtenol were the most abundant compounds in the gut, which are derived from an α-pinene transformation rather than de novo synthesis. Hence, the expression of mevalonate pathway genes in D. rhizophagus gut is not directed to the production of terpenoid pheromones, regardless of their frequent occurrence in the genus Dendroctonus.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

Sarabia

López

Obregón-Molina

et al. 2019

IJMS

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“…Four genes ( DaHMG‐R, DaHMG‐S, DaG/FPPS , and DaIDI ) of D. armandi are regulated by feeding and JH III (Yu et al, 2015). However, as an ongoing effort to characterize other parts of the semiochemical biosynthetic genes in D. armandi , the regulation of AACT, MK, MPDC, PMK , and GGPPS is nesseray for analyzing the formation of informational compounds.…”

Section: Introductionmentioning

confidence: 99%

Isolation and expression of five genes in the mevalonate pathway of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae)

Sun

Dai

Kang

et al. 2020

Arch Insect Biochem Physiol

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The Chinese white pine beetle Dendroctonus armandi (Tsai and Li) is a significant pest of the Qinling and Bashan Mountains pine forests of China. The Chinese white pine beetle can overcome the defences of Chinese white pine Pinus armandi (Franch) through pheromone‐assisted aggregation that results in a mass attack of host trees. We isolated five full‐length complementary DNAs encoding mevalonate pathway‐related enzyme genes from the Chinese white pine beetle (D. armandi), which are acetoacetyl‐CoA thiolase (AACT), geranylgeranyl diphosphate synthase (GGPPS), mevalonate kinase (MK), mevalonate diphosphate decarboxylase (MPDC), and phosphomevalonate kinase (PMK). Bioinformatic analyses were performed on the full‐length deduced amino acid sequences. Differential expression of these five genes was observed between sexes, and within these significant differences among topically applied juvenile hormone III (JH III), fed on phloem of P. armandi, tissue distribution, and development stage. Mevalonate pathway genes expression were induced by JH III and feeding.

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Function of mevalonate pathway genes in the synthesis of frontalin in Chinese white pine beetle,Dendroctonus armandi(curculionidae: Scolytinae)

Sun

Kang

et al. 2021

Arch Insect Biochem Physiol

Self Cite

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The Chinese white pine beetle (Dendroctonus armandi Tsai and Li) is a significant pest of pine forests in the Qinling and Bashan Mountains of China. Adult males commonly produce frontalin using precursors synthesized through the mevalonate pathway, which is regulated by juvenile hormone III (JHIII). In this study, the expression levels of mevalonate pathway genes were quantified after phloem feeding and topical application of the JHIII solution. The frontalin was quantified by gas chromatography-mass spectrometry. Both the phloem feeding and JHIII treatments produced an evident upregulation in the male gut, mainly in 3-hydroxy-3methylglutaryl-CoA synthase (HMGS) and 3-hydroxy-3methylglutaryl-CoA reductase (HMGR). Moreover, HMGS, HMGR, isopentenyl diphosphate isomerase, and geranyl diphosphate synthase/farnesyl diphosphate synthase were upregulated in fed and JHIII-stimulated males of D. armandi under both conditions (solitary and paired). The expression levels were higher in paired compared to solitary males.Males had higher expression levels compared with females. Correspondingly, the phloem-feeding males produced more frontalin than JHIII-treated males, and the production of frontalin was higher in paired males than in solitary males. The knockdown of mevalonate pathway genes using RNAi in vivo effectively reduced the messenger RNA level of these genes and inhibited the production of frontalin. Among them, the silencing of HMGR or HMGS genes reduced the synthesis of frontalin most significantly.

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Isolation and expression of HMG-CoA synthase and HMG-CoA reductase genes in different development stages, tissues and treatments of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae)

Cited by 9 publications

References 38 publications

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle Dendroctonus rhizophagus (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones

Isolation and expression of five genes in the mevalonate pathway of the Chinese white pine beetle, Dendroctonus armandi (Curculionidae: Scolytinae)

Function of mevalonate pathway genes in the synthesis of frontalin in Chinese white pine beetle,Dendroctonus armandi(curculionidae: Scolytinae)

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