Isolation and Expression Analysis of Sucrose Synthase Gene (ScSuSy4) from Sugarcane

Identification of proteins and metabolic networks associated with sucrose accumulation in sugarcane (Saccharum spp. interspecific hybrids)

“…Recognizing the complexity of sugar accumulation in sugarcane, considerable efforts were directed to genomic research, particularly transcriptomics, to unravel the genetic elements and possible genetic regulatory networks of sucrose accumulation in sugarcane (Huang et al 2016;Thirugnanasambandam et al 2017;Chen et al 2019). These studies provided more clarity on molecular processes and genes likely to be involved in sugar metabolism and related biological processes (Reinders et al 2006;Chen et al 2016;Huang et al 2017;Thirugnanasambandam et al 2019;Miao et al 2020). This expanded knowledge, while strengthening the rationale for experimental manipulation of growth and sugar content in sugarcane, also highlights the degree of regulatory complexity inherent in sugarcane stem sucrose accumulation process (Garcia Tavares et al 2018;Chen et al 2019).…”

Analysis of the diversity and tissue specificity of sucrose synthase genes in the long read transcriptome of sugarcane

“…Due to the importance of sucrose in sugarcane, the SuSy genes, especially their identity, gene structure, evolutionary mechanisms and potential functions in sugar and fibre synthesis and accumulation, needs to be well explored. Previous studies suggested the presence of a small gene family encoding different SuSy isoforms within the polyploid sugarcane, but to date characterization of the SuSy genes have not been reported, except for SuSy1 and 2 [15] and SuSy4 [52]. Recently a haplotype identification study of SuSy genes has reported five genes (1–5) in sugarcane [18].…”

Control of sucrose accumulation in sugarcane (Saccharum spp. hybrids) involves miRNA‐mediated regulation of genes and transcription factors associated with sugar metabolism

“…A large number of genes, transcription factors, many known and unknown regulatory factors, such as miRNAs and hormones are involved in sugar metabolism, and starch and sugar accumulation (Wang et al, 2017). Many enzymes and their genes F I G U R E 1 0 Expression levels of genes (left panel) and miRNAs (right panel) by high-throughput sequencing and qRT-PCR involved in sucrose accumulation in sugarcane have been reported (Chen et al, 2016;Grof & Campbell, 2001;Huang et al, 2017;Khanbo et al, 2021;Qin et al, 2021;Thirugnanasambandam et al, 2017;Verma et al, 2019;Wang et al, 2017). MiRNAs are known to regulate numerous growth and development, and metabolic processes in many plants (Chen, Heikkinen, et al, 2019;Saliminejad et al, 2019).…”

“…Over the last few decades considerable efforts were made to understand the mechanistic basis of sucrose accumulation in sugarcane (Chen et al, 2016;Chen, Qin, et al, 2019;Grof & Campbell, 2001;Huang et al, 2017;McCormick et al, 2009;Moore, 2005;Qin et al, 2021;Roopendra et al, 2018;Verma et al, 2019). The theoretical knowledge generated from these studies led to biotechnology efforts, including molecular marker development and transgenics, to improve sucrose accumulation but made little progress so far.…”