Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos

Aamar, Emil; Dawid, Igor B.

doi:10.1387/ijdb.072477ea

Cited by 26 publications

(19 citation statements)

References 36 publications

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“…sox17 and foxj1a are expressed in the zebrafish DFCs, and foxj1a functions to promote cilia formation in various tissues (Aamar and Dawid, 2008;Amack and Yost, 2004;Yu et al, 2008;Tian et al, 2009). We found that sox17 and foxj1a expression levels in DFC -cat1MO , DFC -cat2MO or DFC…”

Section: Dfcs-specific Ctnnb1/2 Activity Regulates Distinct Transcripmentioning

confidence: 73%

β-Catenin 1 and β-catenin 2 play similar and distinct roles in left-right asymmetric development of zebrafish embryos

Zhang

Lin

et al. 2012

Development

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SUMMARY-Catenin-mediated canonical Wnt signaling has been found to be required for left-right (LR) asymmetric development. However, the implication of endogenous -catenin in LR development has not been demonstrated by loss-of-function studies. In zebrafish embryos, two -catenin genes, -catenin 1 (ctnnb1) and -catenin 2 (ctnnb2) are maternally expressed and their zygotic expression occurs in almost all types of tissues, including Kupffer's vesicle (KV), an essential organ that initiates LR development in teleost fish. We demonstrate here that morpholino-mediated knockdown of ctnnb1, ctnnb2, or both, in the whole embryo or specifically in dorsal forerunner cells (DFCs) interrupts normal asymmetry of the heart, liver and pancreas. Global knockdown of ctnnb2 destroys the midline physical and molecular barrier, while global knockdown of ctnnb1 impairs the formation of the midline molecular barrier. Depletion of either gene or both in DFCs/KV leads to poor KV cell proliferation, abnormal cilia formation and disordered KV fluid flow with downregulation of ntl and tbx16 expression. ctnnb1 and ctnnb2 in DFCs/KV differentially regulate the expression of charon, a Nodal antagonist, and spaw, a key Nodal gene for laterality development in zebrafish. Loss of ctnnb1 in DFCs/KV inhibits the expression of charon around KV and of spaw in the posterior lateral plate mesoderm, while ctnnb2 knockdown results in loss of spaw expression in the anterior lateral plate mesoderm with little alteration of charon expression. Taken together, our findings suggest that ctnnb1 and ctnnb2 regulate multiple processes of laterality development in zebrafish embryos through similar and distinct mechanisms.

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Section: Dfcs-specific Ctnnb1/2 Activity Regulates Distinct Transcripmentioning

confidence: 73%

β-Catenin 1 and β-catenin 2 play similar and distinct roles in left-right asymmetric development of zebrafish embryos

Zhang

Lin

et al. 2012

Development

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“…The expression of ciliogenic transcription factor foxj1a is initiated in DFCs, becomes robust towards the end of gastrulation (Aamar and Dawid, 2008;Yu et al, 2008;Tian et al, 2009) and is then gradually downregulated in KV (A.C., unpublished). The correlation between foxj1a expression in DFCs/KV lineage and the temporal requirement for Wnt signaling in KV ciliogenesis prompted us to examine foxj1a transcripts.…”

Section: Non-transgenic Siblings (Gfp-)mentioning

confidence: 99%

“…Two distinct foxj1 genes, foxj1a and foxj1b, have been identified in zebrafish tissues. Their expression is mutually exclusive in KV (foxj1a) and OVs (foxj1b), but partially overlaps in PDs and FP (Aamar and Dawid, 2008;Yu et al, 2008). Depletion of foxj1a results in abnormal LR asymmetry and renal cyst formation, whereas depletion of foxj1b leads to otolith disorganization (Stubbs et al, 2008;Yu et al, 2008;Yu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer’s vesicle

2012

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SUMMARYCilia are essential for normal development. The composition and assembly of cilia has been well characterized, but the signaling and transcriptional pathways that govern ciliogenesis remain poorly studied. Here, we report that Wnt/-catenin signaling directly regulates ciliogenic transcription factor foxj1a expression and ciliogenesis in zebrafish Kupffer's vesicle (KV). We show that Wnt signaling acts temporally and KV cell-autonomously to control left-right (LR) axis determination and ciliogenesis. Specifically, reduction of Wnt signaling leads to a disruption of LR patterning, shorter and fewer cilia, a loss of cilia motility and a downregulation of foxj1a expression. However, these phenotypes can be rescued by KV-targeted overexpression of foxj1a. In comparison to the FGF pathway that has been previously implicated in the control of ciliogenesis, our epistatic studies suggest a more downstream function of Wnt signaling in the regulation of foxj1a expression and ciliogenesis in KV. Importantly, enhancer analysis reveals that KV-specific expression of foxj1a requires the presence of putative Lef1/Tcf binding sites, indicating that Wnt signaling activates foxj1a transcription directly. We also find that impaired Wnt signaling leads to kidney cysts and otolith disorganization, which can be attributed to a loss of foxj1 expression and disrupted ciliogenesis in the developing pronephric ducts and otic vesicles. Together, our data reveal a novel role of Wnt/-catenin signaling upstream of ciliogenesis, which might be a general developmental mechanism beyond KV. Moreover, our results also prompt a hypothesis that certain developmental effects of the Wnt/-catenin pathway are due to the activation of Foxj1 and cilia formation. KEY WORDS: Wnt/-catenin signaling, Ciliogenesis, Foxj1, Kupffer's vesicle ZebrafishWnt/-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle

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“…Among the two paralogous foxj1 genes in the zebrafish genome, foxj1b is expressed in cells of the otic vesicle (Aamar and Dawid, 2008;Tian et al, 2009a;Yu et al, 2008). Transcription of foxj1b initiates as early as 10 hpf, and at this stage is observed throughout the nascent otic placode (Fig.…”

Section: Foxj1b Expression and The Distribution Of Motile Cilia In Thmentioning

confidence: 99%

Cilia-driven fluid flow as an epigenetic cue for otolith biomineralization on sensory hair cells of the inner ear

Lau

et al. 2011

Development

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SUMMARYCiliary motility is necessary for many developmental and physiological processes in animals. In zebrafish, motile cilia are thought to be required for the deposition of otoliths, which comprise crystals of protein and calcium carbonate, on hair cells of the inner ear. The identity of the motile cilia and their role in otolith biogenesis, however, remain controversial. Here, we show that the ear vesicle differentiates numerous motile cilia, the spatial distribution of which changes as a function of the expression pattern of the ciliogenic gene foxj1b. By contrast, the hair cells develop immotile kinocilia that serve as static tethers for otolith crystallization. In ears devoid of all cilia, otoliths can form but they are of irregular shapes and sizes and appear to attach instead to the hair cell apical membranes. Moreover, overproduction of motile cilia also disrupts otolith deposition through sustained agitation of the precursor particles. Therefore, the correct spatial and temporal distribution of the motile cilia is crucial for proper otolith formation. Our findings support the view that the hair cells express a binding factor for the otolith precursors, while the motile cilia ensure that the precursors do not sediment prematurely and are efficiently directed towards the hair cells. We also provide evidence that the kinocilia are modified motile cilia that depend on Foxj1b for their differentiation. We propose that in hair cells, a Foxj1b-dependent motile ciliogenic program is altered by the proneural Atoh proteins to promote the differentiation of immotile kinocilia.

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Isolation and expression analysis of foxj1 and foxj1.2 in zebrafish embryos

Cited by 26 publications

References 36 publications

β-Catenin 1 and β-catenin 2 play similar and distinct roles in left-right asymmetric development of zebrafish embryos

β-Catenin 1 and β-catenin 2 play similar and distinct roles in left-right asymmetric development of zebrafish embryos

Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer’s vesicle

Cilia-driven fluid flow as an epigenetic cue for otolith biomineralization on sensory hair cells of the inner ear

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