Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

Badraiq, Heba; Devito, Liani; Ilić, Duško

doi:10.1007/7651_2014_116

Cited by 13 publications

(10 citation statements)

References 12 publications

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“…Issues such as source of donor tissue, donor age, environmental background, and isolation methods have been described to impact upon the overall quality of these cells (Badraiq et al 2014;Mori et al 2014;Swamynathan et al 2014). However, individual variability is still an unanswered issue, which we sought to address studying UC-MSCs.…”

Section: Discussionmentioning

confidence: 98%

Comparison between isolation protocols highlights intrinsic variability of human umbilical cord mesenchymal cells

et al. 2015

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Mesenchymal stem cells (MSCs) though multipotent exhibit limited lifespan in vitro, with progressive reduction in capacity for self-renewal leading to irreversible arrest of cell division, which limits their use for therapeutic purposes. Human umbilical cord wall MSCs are easy to process and proliferate rapidly in culture, but variability of individual samples and impact upon in vitro expansion and aging processes is unknown. We compared isolation protocols to determine which one yields the highest number of viable cells with the best proliferation capacity. Three different protocols were tested: two were enzymatic procedures and one explant method. Isolated cells were evaluated in terms of proliferation, differentiation capacity, and phenotype. All samples were processed using one or more protocols. After passage 2 adherent cells displayed standard phenotypic and differentiation characteristics of MSCs, but our results show that isolating cells directly from Wharton's jelly is more advantageous. Cells obtained from explants presented similar characteristics to those from enzymatic protocols, but always reached proliferation arrest earlier, irrespective of initial population doubling times. From the same sample, cells obtained with enzymatic protocol ii reached later passages while exhibiting shorter doubling times in culture than cells from other protocols, that is, took longer to reach senescence. More important, each individual MSC sample exhibited different population doubling rates and reached senescence at different passages, irrespective of protocol. Thus, even when in strict conformity with procedures and quality control, each cord sample shows a unique behavior, a finding that should be taken into account when planning for therapeutic approaches.

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Section: Discussionmentioning

confidence: 98%

Comparison between isolation protocols highlights intrinsic variability of human umbilical cord mesenchymal cells

et al. 2015

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“…Previous study has showed that MSCs could not be isolated and expanded in StemPro MSC SFM medium, mTeSR or TheraPEAKTM MSCGM‐CDTM alone, and the addition of human AB serum is needed for the initial isolation . Moreover, high cell seeding densities are needed for MSCs subculture, and lesser density could slow cell proliferation . Some studies have also reported that the isolation, expansion, phenotype and differentiation potential of MSCs are not well supported in MesenCult‐XF .…”

Section: Introductionmentioning

confidence: 99%

Serum and xeno‐free, chemically defined, no‐plate‐coating‐based culture system for mesenchymal stromal cells from the umbilical cord

Kang

Liu

et al. 2016

Cell Proliferation

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“…Collection of the samples was described previously 49 . Briefly, following informed consent, for this study we recruited seven healthy obese pregnant women with BMI ≥ 30 and seven healthy non-obese with BMI between 21 and 25 at the gestational age of 37 weeks (Figure S1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The derivation of MSCs from UC explants and standard cell culture were described in detail previously 1 , 49 . Briefly, the 5–10 mm 2 explants were cultured in DMEM supplemented with 10% fetal calf serum (Hyclone) at 37 °C, 5% CO 2 , 5% O 2 .…”

Section: Methodsmentioning

confidence: 99%

Effects of maternal obesity on Wharton’s Jelly mesenchymal stromal cells

Badraiq

Čvoro

Galleu

et al. 2017

Sci Rep

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We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord’s Wharton’s Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19–25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity. Genome-wide DNA methylation of triple positive (CD73+CD90+CD105+) WJ MSCs found 67 genes with at least one CpG site where the methylation difference was ≥0.2 in four or more obese donors. Only one gene, PNPLA7, demonstrated significant difference on methylome, transcriptome and protein level. Although the number of analysed donors is limited, our data suggest that the altered metabolic environment related to excessive body weight might bear consequences on the WJ MSCs.

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Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

Cited by 13 publications

References 12 publications

Comparison between isolation protocols highlights intrinsic variability of human umbilical cord mesenchymal cells

Comparison between isolation protocols highlights intrinsic variability of human umbilical cord mesenchymal cells

Serum and xeno‐free, chemically defined, no‐plate‐coating‐based culture system for mesenchymal stromal cells from the umbilical cord

Effects of maternal obesity on Wharton’s Jelly mesenchymal stromal cells

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