Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Pelekanos, Rebecca A.; Sardesai, Varda S.; Futrega, Kathryn; Lott, William B.; Kühn, Michael; Doran, Michael

doi:10.3791/54204-v

Cited by 8 publications

(8 citation statements)

References 10 publications

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“…We lack an understanding of the genetic basis of placental-related diseases and so we cannot currently apply CRISPR/Cas9-mediated genome editing to create mutations for disease modelling in existing hTSCs. Instead, we propose that the mRNA transdifferentiation strategy presented here could be applied and further refined to generate iTSCs from patient fibroblasts, or fibroblasts or mesenchymal stromal cells isolated from disease-affected placentas (Pelekanos et al, 2016). This strategy could open up the possibility of generating patient-specific human TSCs.…”

Section: Discussionmentioning

confidence: 99%

Transcription factor-based transdifferentiation of human embryonic to trophoblast stem cells

Fogarty

Abdelbaki

McCarthy

et al. 2021

Preprint

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During the first week of development, human embryos form a blastocyst comprised of an inner cell mass and trophectoderm (TE) cells, the latter of which are progenitors of placental trophoblast. Here we investigated the expression of transcripts in the human TE from early to late blastocyst stages. We identified enrichment of transcription factors GATA2, GATA3, TFAP2C and KLF5 and characterised their protein expression dynamics across TE development. By inducible overexpression and mRNA transfection we determined that these factors, together with MYC, are sufficient to establish induced trophoblast stem cells (iTSCs) from human embryonic stem cells. These iTSCs self-renew and recapitulate morphological characteristics, gene expression profiles and directed differentiation potential similar to existing human TSCs. Systematic omission of each or combinations of factors, revealed the critical importance of GATA2 and GATA3 for successful iTSC reprogramming. Altogether, these findings provide insights into the transcription factor network that may be operational in the human TE and broaden the methods for establishing cellular models of early human placental progenitor cells, which may be useful in the future to model placental-associated diseases.

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Section: Discussionmentioning

confidence: 99%

Transcription factor-based transdifferentiation of human embryonic to trophoblast stem cells

Fogarty

Abdelbaki

McCarthy

et al. 2021

Preprint

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“…The PMSCs and UCMSCs exhibit self-renewability, multipotent differentiation, low immunogenicity, good immunomodulation, an allogenic nature, cytokine secretion, genetic stability and proangiogenic characteristics. 11,[49][50][51] As shown in Fig. 2, MSCs can be isolated from both the fetal and maternal components of placenta.…”

Section: Placenta and Umbilical Cordderived Mscsmentioning

confidence: 99%

“…61 Fetal-origin MSCs exhibit an enhanced proliferation rate, immunosuppressive property, reduction in inflammation, and angiogenenic properties in comparison with maternal-origin MSCs. 50,60 MSCs derived from different anatomical parts of the placenta can be utilized for miscellaneous clinical applications for the treatment of numerous health afflictions.…”

Section: Reviewmentioning

confidence: 99%

“…4 Summarized protocol of recent enzymatic method for stem cell isolation. 50,61 Cell isolation requires tissue wash in collagenase, incubation, enzyme inactivation/filtration, centrifugation, incubation and subculture. The comparative study by Yi et al to isolate MSCs demonstrated superior results with the enzymatic method than explant or perfusion-based isolation.…”

Section: Comparative Study On Human and Animal Placentaderived Mscsmentioning

confidence: 99%

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Human placenta/umbilical cord derivatives in regenerative medicine – Prospects and challenges

et al. 2023

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“…MSCs also can be isolated from neonatal tissue, including human umbilical cord blood (HUCB-MSCs), umbilical cord MSCs (HUC-MSCs), amniotic fluid (HAF-MSCs), placenta MSCs (HP-MSCs), and amniotic membrane (HAM-MSCs), which have been utilized in treating neurological deficits in animal models and patients with intracerebral hemorrhage (ICH) [24][25][26][27][28][29][30]. Recently, a report from a clinical trial found that it is safe and well-tolerated to infuse HUC-MSCs intravenously for moderate and severe coronavirus disease 2019 (COVID- 19) patients, which may be regarded as a promising therapy in targeting the underlying aberrant immune responses [31].…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Mechanisms and Therapeutic Potential of Mesenchymal Stem Cells

Yang

Fan

Liu

et al. 2023

Stem Cell Rev and Rep

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Mesenchymal stem cells (MSCs) are regarded as highly promising cells for allogeneic cell therapy, owing to their multipotent nature and ability to display potent and varied functions in different diseases. The functions of MSCs, including native immunomodulation, high self-renewal characteristic, and secretory and trophic properties, can be employed to improve the immune-modulatory functions in diseases. MSCs impact most immune cells by directly contacting and/or secreting positive microenvironmental factors to influence them. Previous studies have reported that the immunomodulatory role of MSCs is basically dependent on their secretion ability from MSCs. This review discusses the immunomodulatory capabilities of MSCs and the promising strategies to successfully improve the potential utilization of MSCs in clinical research. Graphical Abstract

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Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

Cited by 8 publications

References 10 publications

Transcription factor-based transdifferentiation of human embryonic to trophoblast stem cells

Transcription factor-based transdifferentiation of human embryonic to trophoblast stem cells

Human placenta/umbilical cord derivatives in regenerative medicine – Prospects and challenges

Immunomodulatory Mechanisms and Therapeutic Potential of Mesenchymal Stem Cells

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