Isolation and Diversity of Yeasts from Wild Flowers in Ulleungdo and Yokjido, Korea

Abstract

Various yeasts from wild flowers of Ulleungdo in Gyeongsangbuk-do and Yokjido in Gyeongsangnam-do, Korea were isolated and identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA using BLAST. Forty eight yeast strains of twenty two species and sixty yeast strains of twenty five species were isolated from wild flowers of Ulleungdo and Yokjido, respectively. Only seven species were overlapped from the two different islands areas: Cryptococcus albidus, Cryptococcus laurentii, Metschnikowia reukafii, Pichia scolyti, Rhodotorula glutinis, Rhodotorula graminis and Rhodotorula mucilaginosa. Among forty species from two different islands, other thirty three species were restricted to specific collection site suggesting that each area has distinctive yeast flora.

Characteristics of Two Unrecorded Yeasts from Wild Flowers in Ulleungdo, Korea

“…These results were higher than those of Saccharomyces cerevisiae KCTC 7904 (42.1%) [13], Pichia anomala (31.0%), Pichia anomala KCCM 11473 (72.0%) [19], or Pleurotus cornucopiae (78.0%) [20].…”

“…In a previous paper [13], we reported the diversity of yeasts present on wild flowers in Ulleungdo and Yokjido, Korea; we also reported other characteristics of the unrecorded yeasts isolated from Yokjido [14]. Here, we describe the mycological characteristics of the unrecorded yeasts isolated from Ulleungdo, Korea.…”

“…Most of the yeasts that produce these compounds have been isolated from fermented foods or their raw materials [3,4,6]. Recently, we isolated and identified various yeasts, including unrecorded yeasts, from wild flowers growing in cities [7,8], mountains [9], inland and coastal areas [10,11], and, islands, such as Jeju Island, Korea [12,13].…”

“…We investigated the morphological characteristics of the unrecorded yeasts using previously described methods [13]. The physiological functions of the yeasts were determined as follows.…”

Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea

“…한편, 지금까지 효모는 주로 발효식품 등에서 분리되어 Saccharomyces cerevisiae 등의 일부만이 발효식품산업에 응 용되고 있다. 최근 필자 등은 우리나라 자연 환경의 효모 종 다양성을 확립하고 나아가 국내외 미기록이나 신종 효모를 발굴하기 위하여 야생화에서 1850균주의 효모들을 분리 동 정하였고 43균주의 미기록 효모들을 발굴하여 보고하였다 [10][11][12][13][14][15][16][17][18][19]. 또한 이들 가운데 비병원성 효모들을 선별하여 항 통풍성 xanthine oxidase 저해활성을 보고하였다 [20].…”