Isolation and Distribution of Bacterial Flora in Farmed Rainbow Trout from Mexico

Salgado-Miranda, Celene; Palomares, E. Gabriela; Jurado, Mirsam; Marín, Aída; Vega, Fernando; Soriano‐Vargas, Edgardo

doi:10.1577/h09-004.1

Cited by 19 publications

(16 citation statements)

References 7 publications

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“…To this end, we analysed 253 whitefish samples from five lakes and identified an unprecedented number of bacterial genera in kidneys, including 579 different genera among which 10 were pathogenic bacterial genera that comprised 18 known pathogenic species. This contrasts with previous studies that considered kidneys to be a sterile organ in healthy fish (Goldschmidt‐Clermont et al ., ; Dionne et al ., ; Salgado‐Miranda et al ., ). Of course, one must consider that contamination could partly explain the level of bacterial diversity observed in whitefish kidneys.…”

Section: Discussionmentioning

confidence: 97%

Microbiome investigation in the ecological speciation context of lake whitefish (Coregonus clupeaformis) using next‐generation sequencing

Sevellec

Pavey

Boutin

et al. 2014

J of Evolutionary Biology

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Few studies have applied NGS methods to investigate the microbiome of vertebrates in their natural environment and in freshwater fishes in particularly. Here, we used pyrosequencing of the 16S gene rRNA to (i) test for differences in kidney bacterial communities (i.e. microbiota) of dwarf and normal whitefish found as sympatric pairs, (ii) test the hypothesis of higher bacterial diversity in normal compared with dwarf whitefish and (iii) test for the occurrence of parallelism with the presence and composition of bacterial communities across species pairs inhabiting different lakes. The kidney microbiota of 253 dwarf and normal whitefish from five lakes was analysed combining a double-nested PCR approach with 454 pyrosequencing. Bacteria were detected in 52.6% of the analysed whitefish. There was no overall significant difference among lakes and forms, although the lake 9 form interaction was found significant. We identified 579 bacterial genera, which is substantially more than previous descriptions using less sensitive techniques of fish bacterial diversity in kidney, pathogenic or not. Ten of these genera contained eighteen pathogenic species. Differences in bacteria composition between whitefish forms were not parallel among lakes. In accordance with the higher diversity of prey types, normal whitefish kidney tissue consistently had a more diverse bacterial community and this pattern was parallel among lakes. These results add to building evidence from previous studies on this system that the adaptive divergence of dwarf, and normal whitefish has been driven by both parallel and nonparallel ecological conditions across lakes.

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Section: Discussionmentioning

confidence: 97%

Microbiome investigation in the ecological speciation context of lake whitefish (Coregonus clupeaformis) using next‐generation sequencing

Sevellec

Pavey

Boutin

et al. 2014

J of Evolutionary Biology

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“…fluorescens being one of them [70]. However Pseudomonas species have been identified as members of autochtonous microbiota of farmed fish [71][72][73][74], and several authors have reported the potential probiotic role play for strains of Pseudomonas genus [8,71,75,76].…”

Section: Discussionmentioning

confidence: 99%

Modulation of the Intestinal Microbiota and Immune System of Farmed Sparus aurata by the Administration of the Yeast Debaryomyces hansenii L2 in Conjunction with Inulin

2011

J Aquac Res Development

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Fish gastrointestinal tract is one of the most important sites of interaction with the external world. Interactions between the intestinal microbiota and the host modulate the functionality of the intestinal mucosa and gene expression. Application of probiotics, prebiotics and synbiotics for aquatic animals is increasing to improve welfare and promote growth. However, data about the use of probiotics in conjunction with prebiotics, in farmed marine organisms are scarce. The objective of this work is to study the modulation ability of the intestinal microbiota and the immune system of gilthead seabream exerted by the probiotic yeast Debaromycess hansonii L2 in conjunction with the prebiotic inulin. Fish were fed a commercial diet (control diet I), and with the same diet supplemented with 1.1% D. hansenii strain L2 (10 6 CFU g -1 ) plus inulin (3%) (diet II) for 4 weeks. The whole intestines of healthy fish from each group were aseptically removed at 2 and 4 weeks after starting the experiment and they were analysed by PCR-Denaturing Gradient Gel Electrophoresis (DGGE). Samples of blood and head kidney were obtained for humoral and cellular immune parameters determination. The expression of 12 selected genes related to the immune response (IgM, MHCIα, MHCIIα, C3, IL-1β,TLR, TNFα, CSF-1R, NCCRP-1, Hep, TCRβ y CD8) were analyzed by real-time PCR from skin, intestine, liver and head-kidney tissue.In this study, relevant changes in the intestinal microbiota of gilthead seabream specimens fed the diets assayed has been demonstrated. An important effect on the intestinal microbiota by the dietary administration of a synbiotic mixture has also been detected, especially in fish receiving the supplemented diet for 4 weeks. These changes coincided in the same time with an up-regulation of the expression of immunological genes in skin and head kidney of gilthead seabream at 2 and 4 weeks, respectively.

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“…A large number of freshwater species harbor plesiomonads. In addition to those fish cited in a previous review (28), recent investigations have isolated Plesiomonas from rainbow trout (53,54), carp (55), and tilapia (44,56,57). Studies of the intestinal tracts of a number of freshwater fish suggest that the genus Plesiomonas is one of the most common species composing the bacterial microbiota of these vertebrates, in addition to Fusobacterium and Aeromonas (58,59).…”

Section: Invertebrate and Vertebrate Hostsmentioning

confidence: 99%

Plesiomonas shigelloides Revisited

Janda¹,

2016

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SUMMARYAfter many years in the familyVibrionaceae, the genusPlesiomonas, represented by a single species,P. shigelloides, currently resides in the familyEnterobacteriaceae, although its most appropriate phylogenetic position may yet to be determined. Common environmental reservoirs for plesiomonads include freshwater ecosystems and estuaries and inhabitants of these aquatic environs. Long suspected as being an etiologic agent of bacterial gastroenteritis, convincing evidence supporting this conclusion has accumulated over the past 2 decades in the form of a series of foodborne outbreaks solely or partially attributable toP. shigelloides. The prevalence ofP. shigelloidesenteritis varies considerably, with higher rates reported from Southeast Asia and Africa and lower numbers from North America and Europe. Reasons for these differences may include hygiene conditions, dietary habits, regional occupations, or other unknown factors. Other human illnesses caused byP. shigelloidesinclude septicemia and central nervous system disease, eye infections, and a variety of miscellaneous ailments. For years, recognizable virulence factors potentially associated withP. shigelloidespathogenicity were lacking; however, several good candidates now have been reported, including a cytotoxic hemolysin, iron acquisition systems, and lipopolysaccharide. WhileP. shigelloidesis easy to identify biochemically, it is often overlooked in stool samples due to its smaller colony size or relatively low prevalence in gastrointestinal samples. However, one FDA-approved PCR-based culture-independent diagnostic test system to detect multiple enteropathogens (FilmArray) includesP. shigelloideson its panel. Plesiomonads produce β-lactamases but are typically susceptible to many first-line antimicrobial agents, including quinolones and carbapenems.

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Isolation and Distribution of Bacterial Flora in Farmed Rainbow Trout from Mexico

Cited by 19 publications

References 7 publications

Microbiome investigation in the ecological speciation context of lake whitefish (Coregonus clupeaformis) using next‐generation sequencing

Microbiome investigation in the ecological speciation context of lake whitefish (Coregonus clupeaformis) using next‐generation sequencing

Modulation of the Intestinal Microbiota and Immune System of Farmed Sparus aurata by the Administration of the Yeast Debaryomyces hansenii L2 in Conjunction with Inulin

Plesiomonas shigelloides Revisited

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