Isolation and differential secretion of metalloproteinase by superficial chondrocytes in articular cartilage

Takagi, Toshitaka; Takahashi, Saki; Koshino, Tomihisa; Okamoto, Ryo; Jasin, Hugo E.

doi:10.1007/s002640050208

Cited by 6 publications

(8 citation statements)

References 9 publications

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“…Previous studies have also reported similar aggrecan expression levels between SZC and DZC but have documented that SZC produce less PG than DZC, because of increased activity of matrix degradation enzymes (Darling et al 2004;Darling and Athanasiou 2005;Hauselmann et al 1996;Takagi et al 1998;Reid et al 2000). Our findings suggest a relative deficiency in this degradative activity in adult versus immature SZC, as PG amounts are higher in cultures of adult SZC than in immature SZC or adult DZC, despite similar aggrecan gene expression levels in all three groups.…”

Section: Zonal Stratification Of Articular Chondrocytessupporting

confidence: 72%

Maturational differences in superficial and deep zone articular chondrocytes

et al. 2005

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To examine whether differences in chondrocytes from skeletally immature versus adult individuals are important in cartilage healing, repair, or tissue engineering, superficial zone chondrocytes (SZC, from within 100 microm of the articular surface) and deep zone chondrocytes (DZC, from 30%-45% of the deepest un-mineralized part of articular cartilage) were harvested from immature (1-4 months) and young adult (18-36 months) steers and compared. Cell size, matrix gene expression and protein levels, integrin levels, and chemotactic ability were measured in cells maintained in micromass culture for up to 7 days. Regardless of age, SZC were smaller, had a lower type II to type I collagen gene expression ratio, and higher gene expression of SZ proteins than their DZC counterparts. Regardless of zone, chondrocytes from immature steers had higher levels of Sox 9 and type II collagen gene expression. Over 7 days in culture, the SZC of immature steers had the highest rate of proliferation. Phenotypically, the SZC of immature and adult steers were more stable than their respective DZC. Cell surface alpha5 and alpha2 integrin subunit levels were higher in the SZC of immature than of adult steers, whereas beta1 integrin subunit levels were similar. Both immature and adult SZC were capable of chemotaxis in response to fetal bovine serum or basic fibroblast growth factor. Our data indicate that articular chondrocytes vary in the different zones of cartilage and with the age of the donor. These differences may be important for cartilage growth, tissue engineering, and/or repair.

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Section: Zonal Stratification Of Articular Chondrocytessupporting

confidence: 72%

Maturational differences in superficial and deep zone articular chondrocytes

et al. 2005

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“…Furthermore, a number of studies have reported that the superficial chondrocytes synthesise •NO at a greater rate than deep zone cells in response to IL-1β [19][20][21] . Moreover, it has been demonstrated recently that •NO mediates the inhibition of proteoglycan synthesis in chondrocyte sub-populations [22][23][24][25] .…”

Section: Introductionmentioning

confidence: 99%

Dynamic compression counteracts IL-1β-induced release of nitric oxide and PGE2by superficial zone chondrocytes cultured in agarose constructs

Chowdhury

Bader

Lee

2003

Osteoarthritis and Cartilage

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“…The smaller chondrocytes consisted of a superficial layer of articular cartilage and the larger consisted of a deeper layer of articular cartilage. 11,36 The chondrocytes in both subpopulations expressed CD44 in suspension culture.…”

Section: Discussionmentioning

confidence: 99%

“…[32][33][34][35] Superficial chondrocytes secreted greater amounts of metalloproteinase 36 and NO 34 compared to chondrocytes obtained from the deeper layer of cartilage. Fibronectin synthesis by superficial chondrocytes was less than that by deep chondrocytes, and superficial chondrocytes had protective ability in the early phase of inflammatory arthritis.…”

Section: Discussionmentioning

confidence: 99%

Upregulation of CD44 expression on bovine articular chondrocytes induced by synthetic lipid A

Sato

Takagi

Okamoto

et al. 2002

Modern Rheumatology

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The purpose of the study reported in this article was to investigate effects of synthetic lipid A on the expression of adhesion molecule CD44 on bovine articular chondrocytes. Full-thickness bovine articular cartilage was dissected from the carpometacarpal joints of 24 cows. Cartilage pieces were enzymatically digested to liberate chondrocytes. The chondrocytes were incubated in the presence of synthetic lipid A in suspension culture. Cell characteristics and binding of monoclonal antihuman CD44 antibodies were assessed with a flow cytometer. The expression of CD44 mRNA in chondrocytes was detected by reverse transcription-polymerase chain reaction (RT-PCR) technique. PCR products were quantified with a chargecoupled device image sensor. The percentage of CD44positive chondrocytes was 42.2% Ϯ 12.0%, 51.7% Ϯ 6.8%, and 51.1% Ϯ 5.0%, in the presence of lipid A at 0.25 µg/ml, 2.5 µg/ml, and 25 µg/ml, respectively, whereas it was 39.2% Ϯ 8.9% in the absence of lipid A. In flow cytometry, two subpopulations of chondrocytes were found in each of five separate experiments, one with smaller number of forward scatter (FS) and the other with larger number of FS. The percentage of CD44-positive cells was 24.8% Ϯ 8.5% in the subpopulation with smaller number of FS and 31.9% Ϯ 6.4% in the subpopulation with larger number of FS at time 24 h after incubation. The bacterial component, lipid A, upregulated expression of CD44 on articular chondrocytes.

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Isolation and differential secretion of metalloproteinase by superficial chondrocytes in articular cartilage

Cited by 6 publications

References 9 publications

Maturational differences in superficial and deep zone articular chondrocytes

Maturational differences in superficial and deep zone articular chondrocytes

Dynamic compression counteracts IL-1β-induced release of nitric oxide and PGE2by superficial zone chondrocytes cultured in agarose constructs

Upregulation of CD44 expression on bovine articular chondrocytes induced by synthetic lipid A

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