Isolation and Determination of Bile Salt Hydrolase-Producing Lactic Acid Bacteria from Fermented Spider Plant

Yasiri, Atipat; Vannaxay, Emilie; Kiatmontri, Jinatta; Seubsasana, Supawadee

doi:10.22207/jpam.12.3.03

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…These effects have been confirmed by clinical studies [ 32 ]. BSH activity is related to the cholesterol-lowering potential of LAB, and its presence has been demonstrated in many strains of L. plantarum , including the strain 299v [ 9 , 33 , 34 , 35 , 36 ]. Several studies on BSH substrate specificity have shown substrate preference toward glycine-conjugated bile salts [ 10 , 12 , 37 , 38 ].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

Hernández-Gómez

López-Bonilla

Trejo‐Tapia

et al. 2021

Foods

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Bile salt hydrolase (BSH) activity in probiotic strains is usually correlated with the ability to lower serum cholesterol levels in hypercholesterolemic patients. The objective of this study was the evaluation of BSH in five probiotic strains of lactic acid bacteria (LAB) and a probiotic yeast. The activity was assessed using a qualitative direct plate test and a quantitative high-performance thin- layer chromatography assay. The six strains differed in their BSH substrate preference and activity. Lactobacillus plantarum DGIA1, a potentially probiotic strain isolated from a double cream cheese from Chiapas, Mexico, showed excellent deconjugation activities in the four tested bile acids (69, 100, 81, and 92% for sodium glycocholate, glycodeoxycholate, taurocholate, and taurodeoxycholate, respectively). In the case of the commercial probiotic yeast Saccharomyces boulardii, the deconjugation activities were good against sodium glycodeoxycholate, taurocholate, and taurodeoxycholate (100, 57, and 63%, respectively). These last two results are part of the novelty of the work. A weak deconjugative activity (5%) was observed in the case of sodium glycocholate. This is the first time that the BSH activity has been detected in this yeast.

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Section: Resultsmentioning

confidence: 99%

In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

Hernández-Gómez

López-Bonilla

Trejo‐Tapia

et al. 2021

Foods

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“…On the other hand, BSH activity has been found primarily in the intestinal bacteria of several genera, including Bacteroides , Bifidobacterium , Clostridium , Enterococcus , and Lactobacillus . In contrast, no BSH activity has been observed in bacteria isolated from bile salt-free environments ( Jones et al, 2008 ), except for a few recent cases ( Yasiri et al, 2018 ; Nuhwa et al, 2019 ; Singhal et al, 2019 ). For instance, AtPVA and PaPVA (penicillin acylases) from A. tumefaciens and Pectobacterium atrosepticum , respectively, inhabiting plants and soils unlikely to contain bile salts did not show any BSH activity ( Sunder et al, 2017 ; Daly et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Bile Salt Hydrolase Degrades β-Lactam Antibiotics and Confers Antibiotic Resistance on Lactobacillus paragasseri

et al. 2022

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Bile salt hydrolase (BSH) is a well-characterized probiotic enzyme associated with bile detoxification and colonization of lactic acid bacteria in the human gastrointestinal tract. Here, we isolated a putative BSH (LpBSH) from the probiotic bacterium Lactobacillus paragasseri JCM 5343T and demonstrated its bifunctional activity that allows it to degrade not only bile salts but also the antibiotic (penicillin). Although antibiotic resistance and bile detoxification have been separately recognized as different microbial functions, our findings suggest that bifunctional BSHs simultaneously confer ecological advantages to host gut bacteria to improve their survival in the mammalian intestine by attaining a high resistance to bile salts and β-lactams. Strain JCM 5343T showed resistance to both bile salts and β-lactam antibiotics, suggesting that LpBSH may be involved in this multi-resistance of the strain. We further verified that such bifunctional enzymes were broadly distributed among the phylogeny, suggesting that the bifunctionality may be conserved in other BSHs of gut bacteria. This study revealed the physiological role and phylogenetic diversity of bifunctional enzymes degrading bile salts and β-lactams in gut bacteria. Furthermore, our findings suggest that the hitherto-overlooked penicillin-degrading activity of penicillin acylase could be a potential new target for the probiotic function of gut bacteria.

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“…Ten antibiotics, which included ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, nalidixic acid, novobiocin, penicillin, tetracycline and vancomycin, were selected to examine the susceptibility profile of the isolate. The procedure was done following Yasiri et al 19…”

Section: Antibiotic Susceptibility Profilementioning

confidence: 99%

Gamma-aminobutyric Acid (GABA) Producing Probiotic Lactiplantibacillus Pentosus Isolated from Fermented Spider Plant (Pak Sian Dong) in Thailand

Amatachaya¹,

Siramolpiwat²,

Kraisorn³

et al. 2023

J. Pure Appl. Microbiol.

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Psychobiotics are defined as the organisms that can provide the mental health benefit. The possible mechanism of psychobiotics is manipulation of neurotransmitter production and neurotransmitter production by the microbes. The lactobacillus group has been reported for the potential of neurotransmitter production, especially g-aminobutyric acid (GABA) which is an important inhibitory neurotransmitter. Therefore, GABA can be used for relaxation and applied in various psychiatric disorders. The aim of this study was determination of lactic acid bacterial isolates from Pak Sian Dong in Thailand for GABA producing ability. The results found that there were 3 isolates, SF66, SF80 and SF82, which revealed the ability to produce glutamic acid decarboxylase (GAD) enzyme. The GABA were detected by thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC) in the bacterial culture containing 3% monosodium glutamate. The survival in gastrointestinal synthetic condition found that only SF66 isolate showed the authentic percentage of survival then this isolate was selected. From the identification, the isolate was identified as Lactiplantibacillus pentosus and was designated as L. pentosus SF66 which exhibited with the potential for further investigation and development to be psychobiotics.

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Isolation and Determination of Bile Salt Hydrolase-Producing Lactic Acid Bacteria from Fermented Spider Plant

Cited by 6 publications

References 35 publications

In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

In Vitro Bile Salt Hydrolase (BSH) Activity Screening of Different Probiotic Microorganisms

Bile Salt Hydrolase Degrades β-Lactam Antibiotics and Confers Antibiotic Resistance on Lactobacillus paragasseri

Gamma-aminobutyric Acid (GABA) Producing Probiotic Lactiplantibacillus Pentosus Isolated from Fermented Spider Plant (Pak Sian Dong) in Thailand

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