“…The number of shoots was reported stagnant on MS medium supplemented with Kin (0.5 mg/L), even after four months of incubation. Generally, ferns micropropagated using spores in most of the studies like Salvinia natans [32], Dryopteris affinis [33], Equisetum arvense [34], Marsilea minuta [35], and Pteris tripartita [36], but the shoots were induced from the nodal part of the rhizomes in present study. It has been reported that the supplementation of abscisic acid in the medium induced shoots, roots, and elongation of petioles of M. quadrifolia [37,38], whereas the supplementation of auxins has no ameliorative effects on the regenerative response in this plant species [28].…”