Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region

Ricca, Alessandra; Cascino, Federica; Gritti, Angela

doi:10.1007/978-1-0716-1783-0_2

Cited by 2 publications

(2 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NSCs can undergo self-renewal replication or differentiate into the main neural cell types: neurons, astrocytes, and oligodendrocytes. These cells grow in vitro as neurospheres (Figure 1), and their propagation in culture takes place in absence of serum and in presence of EGF and bFGF [13]. Moreover, they can differentiate into various lineages (e.g., neurons, astrocytes, and oligodendrocytes) in adhesion (Figure 3), in the presence of low serum concentration (1-2%).…”

Section: Neural Stem Cellsmentioning

confidence: 99%

Two Sides of The Same Coin: Normal and Tumoral Stem Cells, The Relevance of In Vitro Models and Therapeutic Approaches: The Experience with Zika Virus in Nervous System Development and Glioblastoma Treatment

Tinnirello,

Chinnici,

Miceli

et al. 2023

IJMS

View full text Add to dashboard Cite

Neural stem cells (NSCs) were described for the first time more than two decades ago for their ability to differentiate into all neural cell lineages. The isolation of NSCs from adults and embryos was carried out by various laboratories and in different species, from mice to humans. Similarly, no more than two decades ago, cancer stem cells were described. Cancer stem cells, previously identified in hematological malignancies, have now been isolated from several solid tumors (breast, brain, and gastrointestinal compartment). Though the origin of these cells is still unknown, there is a wide consensus about their role in tumor onset, propagation and, in particular, resistance to treatments. Normal and neoplastic neural stem cells share common characteristics, and can thus be considered as two sides of the same coin. This is particularly true in the case of the Zika virus (ZIKV), which has been described as an inhibitor of neural development by specifically targeting NSCs. This understanding prompted us and other groups to evaluate ZIKV action in glioblastoma stem cells (GSCs). The results indicate an oncolytic activity of this virus vs. GSCs, opening potentially new possibilities in glioblastoma treatment.

show abstract

Section: Neural Stem Cellsmentioning

confidence: 99%

Two Sides of The Same Coin: Normal and Tumoral Stem Cells, The Relevance of In Vitro Models and Therapeutic Approaches: The Experience with Zika Virus in Nervous System Development and Glioblastoma Treatment

Tinnirello,

Chinnici,

Miceli

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Starting from fixed tissue, we can define NSCs based on their intracellular and membrane marker expression. The immunohistochemical identification of human NSCs, particularly those in the hippocampus, involves neurofilament markers such as nestin, doublecortin, neuronal nuclei protein, β-tubulin 3, light neurofilament polypeptide, microtubule-associated proteins (e.g., MAP1b/2/5) [ 18 , 19 , 20 , 21 ], and cell proliferation proteins (e.g., Ki67) [ 20 ] known to be present during relatively early neuronal development stages.…”

Section: Neural Stem Cellsmentioning

confidence: 99%

NSC Physiological Features in Spinal Muscular Atrophy: SMN Deficiency Effects on Neurogenesis

Adami

Bottai

2022

IJMS

View full text Add to dashboard Cite

While the U.S. Food and Drug Administration and the European Medicines Evaluation Agency have recently approved new drugs to treat spinal muscular atrophy 1 (SMA1) in young patients, they are mostly ineffective in older patients since many motor neurons have already been lost. Therefore, understanding nervous system (NS) physiology in SMA patients is essential. Consequently, studying neural stem cells (NSCs) from SMA patients is of significant interest in searching for new treatment targets that will enable researchers to identify new pharmacological approaches. However, studying NSCs in these patients is challenging since their isolation damages the NS, making it impossible with living patients. Nevertheless, it is possible to study NSCs from animal models or create them by differentiating induced pluripotent stem cells obtained from SMA patient peripheral tissues. On the other hand, therapeutic interventions such as NSCs transplantation could ameliorate SMA condition. This review summarizes current knowledge on the physiological properties of NSCs from animals and human cellular models with an SMA background converging on the molecular and neuronal circuit formation alterations of SMA fetuses and is not focused on the treatment of SMA. By understanding how SMA alters NSC physiology, we can identify new and promising interventions that could help support affected patients.

show abstract

Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region

Cited by 2 publications

References 53 publications

Two Sides of The Same Coin: Normal and Tumoral Stem Cells, The Relevance of In Vitro Models and Therapeutic Approaches: The Experience with Zika Virus in Nervous System Development and Glioblastoma Treatment

Two Sides of The Same Coin: Normal and Tumoral Stem Cells, The Relevance of In Vitro Models and Therapeutic Approaches: The Experience with Zika Virus in Nervous System Development and Glioblastoma Treatment

NSC Physiological Features in Spinal Muscular Atrophy: SMN Deficiency Effects on Neurogenesis

Contact Info

Product

Resources

About