Isolation and Culture of Human Dermal Microvascular Endothelial Cells

Bourland, Jennifer; Mayrand, Dominique; Tremblay, Nathalie; Moulin, Véronique; Fradette, Julie; Auger, François A.

doi:10.1007/978-1-4939-9473-1_7

Cited by 15 publications

(17 citation statements)

References 21 publications

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“…Thus, EC proliferation decreased in the hyperglycemic condition through reduced expression of ICAM-1. These findings established that increased EC proliferation is a major step in injury repair (Liang et al, 2007;Bourland et al, 2019). Given our observation that HG reduced EC proliferation via ICAM-1, we hypothesized that low levels of ICAM expression would decrease injury closure in the HG group.…”

Section: Hg Decreases Ec Proliferation By Reducing Icam-1 Expressionmentioning

confidence: 71%

“…Recent studies have shown that the interaction of LFA-1 with its ligand ICAM-1 mediates several important steps in the cell immune response. For example, LFA-1 integrin is critical for the firm adhesion of neutrophils to ICAM-1 (Meisel et al, 2018) and the expression of ICAM-1 and LFA-1 triggers the activation of myosin light chains, MAPK and Rho GTPase, which enhances neutrophil transmigration into inflamed tissues (Wolcott et al, 2016;Bourland et al, 2019). Neutrophil recruitment has been associated with bacterial clearance at injury sites.…”

Section: Discussionmentioning

confidence: 99%

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Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1 Expression Levels

et al. 2020

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Delayed repair is a serious public health concern for diabetic populations. Intercellular adhesion molecule 1 (ICAM-1) and Lymphocyte function-associated antigen 1 (LFA-1) play important roles in orchestrating the repair process. However, little is known about their effects on endothelial cell (EC) proliferation and neutrophil activity in subjects with hyperglycemia (HG). We cultured ECs and performed a scratch-closure assay to determine the relationship between ICAM-1 and EC proliferation. Specific internally labeled bacteria were used to clarify the effects of ICAM-1 and LFA-1 on neutrophil phagocytosis. Transwell assay and fluorescence-activated cell sorting analysis evaluated the roles of ICAM-1 and LFA-1 in neutrophil recruitment. ICAM-1+/+ and ICAM-1–/– mice were used to confirm the findings in vivo. The results demonstrated that HG decreased the expression of ICAM-1, which lead to the low proliferation of ECs. HG also attenuated neutrophil recruitment and phagocytosis by reducing the expression of ICAM-1 and LFA-1, which were strongly associated with the delayed repair.

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Section: Hg Decreases Ec Proliferation By Reducing Icam-1 Expressionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1 Expression Levels

et al. 2020

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“…Due to technical difficulty in isolation and culture of human dermal microvascular endothelial cells (HDMECs), 19‐22 currently immunohistochemistry in skin tissue 23,24 and human umbilical vein endothelial cells 25,26 are major approaches in the study of pathogenesis of psoriasis. In the present study, we isolated HDMECs from the human dermis and compared the functions, including angiogenesis, migration and metabolism, of HDMECs from psoriatic lesions vs. that from the skin of healthy subjects.…”

Section: Introductionmentioning

confidence: 99%

Increased angiogenesis and migration of dermal microvascular endothelial cells from patients with psoriasis

Hou

Zhou

et al. 2021

Experimental Dermatology

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Psoriasis displays both increased angiogenesis and microvascular dilation in the skin, while human dermal microvascular endothelial cells (HDMECs) are involved in angiogenesis and microvascular dilation. Whether the functions of HDMECs are altered in psoriatic skin versus healthy skin remain unknown. Here, we isolated HDMECs from the skin of 10 patients with psoriasis and 10 healthy subjects and compared angiogenesis, proliferation, migration and cell metabolism between psoriatic HDMECs and normal HDMECs. We found that the morphology of primary HDMECs was comparable between psoriatic HDMECs and normal HDMECs. After passage, psoriatic HDMECs displayed larger cell size and wider intercellular space. In addition to DiI‐Ac‐LDL (DiI‐labelled acetylated low‐density lipoprotein) uptake, expression levels of CD31, vWF (von Willebrand factor) and LYVE‐1 were comparable in psoriatic HDMECs versus normal HDMECs. However, psoriatic HDMECs exhibited increased tube formation (numbers of nodes and meshes, p < 0.05) and migration (numbers of migrated cells, p < 0.001) and reductions in proliferation (growth rates, p < 0.05) and energy metabolism (oxygen consumption rate and extracellular acidification rate, p < 0.05) compared with normal HDMECs. Therefore, psoriatic HDMECs display an increased angiogenesis and migration and decreased proliferation and metabolic activity, suggesting a pathogenic role of HDMECs in psoriasis.

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“…The underlying hypothesis was that grafted tissues that are more rapidly vascularized will not suffer from alterations due to a lack of oxygen and nutrients. To generate tissue-engineered constructs with microvascular networks, the first step is to have access to endothelial cells, which can be isolated from human umbilical vein, neonatal foreskin, and adult skin (reviewed in [108]). Then, endothelial cells must be cocultured with stromal cells or added to the cell sheets during the maturation phase ( Figure 1(c)).…”

Section: Addition Of Vascular and Lymphatic Microvascularmentioning

confidence: 99%

Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors

Roy

Magne

Vaillancourt-Audet

et al. 2020

BioMed Research International

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Cancer research has considerably progressed with the improvement of in vitro study models, helping to understand the key role of the tumor microenvironment in cancer development and progression. Over the last few years, complex 3D human cell culture systems have gained much popularity over in vivo models, as they accurately mimic the tumor microenvironment and allow high-throughput drug screening. Of particular interest, in vitrohuman 3D tissue constructs, produced by the self-assembly method of tissue engineering, have been successfully used to model the tumor microenvironment and now represent a very promising approach to further develop diverse cancer models. In this review, we describe the importance of the tumor microenvironment and present the existing in vitro cancer models generated through the self-assembly method of tissue engineering. Lastly, we highlight the relevance of this approach to mimic various and complex tumors, including basal cell carcinoma, cutaneous neurofibroma, skin melanoma, bladder cancer, and uveal melanoma.

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Isolation and Culture of Human Dermal Microvascular Endothelial Cells

Cited by 15 publications

References 21 publications

Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1 Expression Levels

Hyperglycemia Decreases Epithelial Cell Proliferation and Attenuates Neutrophil Activity by Reducing ICAM-1 and LFA-1 Expression Levels

Increased angiogenesis and migration of dermal microvascular endothelial cells from patients with psoriasis

Human Organ-Specific 3D Cancer Models Produced by the Stromal Self-Assembly Method of Tissue Engineering for the Study of Solid Tumors

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