Isolation and Cultivation of Anaerobes

Börner, Rosa Aragão

doi:10.1007/10_2016_1

Cited by 13 publications

(12 citation statements)

References 81 publications

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“…To prove whether-potential periodontal pathogens could be causal for LVAD-related infections, other localizations and the examined bacteria would need to be investigated. First, the above-mentioned bacteria related to periodontitis are anaerobic, showing a complex metabolism and special cultivation requirements [23]. Second, the common potential periodontal pathogens or their DNA were found in different cardiac tissue [24,25].…”

Section: Discussionmentioning

confidence: 99%

No Association between Clinical Periodontal Conditions and Microbiological Findings on Driveline of Patients with Left-Ventricular Assist Devices (LVAD)

et al. 2021

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The aim of this retrospective study was to investigate whether there would be an association between periodontal disease parameters and positive bacterial findings at the driveline of patients with a left ventricular assist device (LVAD). Patients with an LVAD, who underwent a full oral and microbiological examination between 2016 and 2018, were included. During oral examination, periodontitis severity (stage and grade) and the periodontal inflamed surface area (PISA) were evaluated. A microbiological analysis was performed from swabs of the driveline, whereby different bacterial species were cultivated and analyzed. A total of 73 patients were included in the current study. The majority of participants (80.8%) had at least one positive bacterial finding during the study period. Most patients had a periodontitis stage of III-IV (80.9%). The determined PISA of the total group was 284.78 ± 352.29 mm². No associations were found between the periodontal disease parameters and the bacterial findings in general, the bacterial findings on the day of oral examination or the bacterial findings 12 months prior to/after the oral examination (p > 0.05). Periodontitis is not associated with cultivated microbiological findings at the driveline of patients with an LVAD and thus appears not to be a risk indicator for driveline colonization. Nevertheless, the high periodontal burden in LVAD patients underlines the need for their improved periodontal care.

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Section: Discussionmentioning

confidence: 99%

No Association between Clinical Periodontal Conditions and Microbiological Findings on Driveline of Patients with Left-Ventricular Assist Devices (LVAD)

et al. 2021

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“…Although the accuracy and ease of functional gene prediction is rapidly improving, advances in functional assignment of microbial-glycan interactions have been limited. This is because cultivation can be difficult and low throughput (Alain and Querellou, 2009;Börner, 2016), even with the development of culturomic techniques [e.g., iChip (Berdy et al, 2017)]. Recently, phenotype-based methods have gained popularity because they enable functional screening of microbial cells in complex microbial communities.…”

Section: Next-generation Physiology To Identify the Function Of Single Cells Visualizing Microbiome-carbohydrate Interactions With Carbohmentioning

confidence: 99%

Approaches to Investigate Selective Dietary Polysaccharide Utilization by Human Gut Microbiota at a Functional Level

et al. 2021

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The human diet is temporally and spatially dynamic, and influenced by culture, regional food systems, socioeconomics, and consumer preference. Such factors result in enormous structural diversity of ingested glycans that are refractory to digestion by human enzymes. To convert these glycans into metabolizable nutrients and energy, humans rely upon the catalytic potential encoded within the gut microbiome, a rich collective of microorganisms residing in the gastrointestinal tract. The development of high-throughput sequencing methods has enabled microbial communities to be studied with more coverage and depth, and as a result, cataloging the taxonomic structure of the gut microbiome has become routine. Efforts to unravel the microbial processes governing glycan digestion by the gut microbiome, however, are still in their infancy and will benefit by retooling our approaches to study glycan structure at high resolution and adopting next-generation functional methods. Also, new bioinformatic tools specialized for annotating carbohydrate-active enzymes and predicting their functions with high accuracy will be required for deciphering the catalytic potential of sequence datasets. Furthermore, physiological approaches to enable genotype-phenotype assignments within the gut microbiome, such as fluorescent polysaccharides, has enabled rapid identification of carbohydrate interactions at the single cell level. In this review, we summarize the current state-of-knowledge of these methods and discuss how their continued development will advance our understanding of gut microbiome function.

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“…Cultivation of anaerobes may be performed to propagate the microorganism of interest and to produce metabolic end products. Anaerobic microorganism can be found in a wide range of environments (Börner 2016 ). An organism can be classified with respect to the energy source, the electron donor species/compound and the carbon source it uses.…”

Section: Anaerobic Microbiology and Biomass Cultivation Techniquesmentioning

confidence: 99%

Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology

et al. 2018

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Anaerobic microorganisms (anaerobes) possess a fascinating metabolic versatility. This characteristic makes anaerobes interesting candidates for physiological studies and utilizable as microbial cell factories. To investigate the physiological characteristics of an anaerobic microbial population, yield, productivity, specific growth rate, biomass production, substrate uptake, and product formation are regarded as essential variables. The determination of those variables in distinct cultivation systems may be achieved by using different techniques for sampling, measuring of growth, substrate uptake, and product formation kinetics. In this review, a comprehensive overview of methods is presented, and the applicability is discussed in the frame of anaerobic microbiology and biotechnology.

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Isolation and Cultivation of Anaerobes

Cited by 13 publications

References 81 publications

No Association between Clinical Periodontal Conditions and Microbiological Findings on Driveline of Patients with Left-Ventricular Assist Devices (LVAD)

No Association between Clinical Periodontal Conditions and Microbiological Findings on Driveline of Patients with Left-Ventricular Assist Devices (LVAD)

Approaches to Investigate Selective Dietary Polysaccharide Utilization by Human Gut Microbiota at a Functional Level

Methods for quantification of growth and productivity in anaerobic microbiology and biotechnology

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