Isolation and complete nucleotide sequence of the gene for bovine parathyroid hormone

Weaver, Christine A.; Gordon, David F.; Kissil, M.S.; Mead, David A.; Kemper, Byron

doi:10.1016/0378-1119(84)90149-5

Cited by 62 publications

(17 citation statements)

References 20 publications

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“…Confirmation of the meat species was according to Wolf et al [10]. Design of primers was based on the bovine Pth gene (K01938) and a 12S rRNA gene [8,21]. Selected primers, (5 0 -tataaaagtcacattgaagggtctacag-3 0 and 5 0 -tgtaagaaagaactcatggaaacttaaa-3 0 for the Pth gene and (a/g)cttaccttgttac-3 0 for the 12SrRNA gene [11], were based on criteria of specific DNA amplification to Pth gene and 12S rRNA gene without leaving non-specific products.…”

Section: Methodsmentioning

confidence: 99%

“…15) were described in Table 2. Since, it was previously addressed that the Pth gene is single copy per haploid genome [21], the relationship between a target gene and its corresponding cell numbers was then estimated based on the fact that each bovine cell contained two copies of DNAs per diploid (single copy per haploid) whereas total cell numbers of both bovine and non-bovine were estimated from the 12S rRNA genes. The ratios between copy number of Pth genes and total 12S rRNAs in the experiment were used to estimate the bovine content in the samples the same way applied to content determination among genetically modified foods [12,19].…”

Section: Quantitative Analysis Of Bovine Contents In Feedstuffsmentioning

confidence: 99%

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Novel electrochemical identification and semi quantification of bovine constituents in feedstuffs

Chaumpluk

Chikae

Takamura

et al. 2006

Science and Technology of Advanced Materials

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Identifying contaminating bovine constituents in feed has been a major means to help prevent the spread of bovine spongiform encephalopathy (BSE). The phenomenon of DNA aggregation induced by Hoechst 33258 in conjunction with the change in anodic current measurement was, for the first time, applied for bovine DNA detection in feedstuffs. By using the PCR amplification system specific to bovine parathyroid and common 12S rRNA genes, anodic current peaks measurements of these PCR products on linear sweep voltammetry could be determined. Anodic peaks measurement of bovine parathyroid gene among ruminant meat and pet foods containing bovine constituents were at 1.18-1.52 mA while anodic peaks among non bovine samples were greater than 1.78 mA. In the study, anodic current peaks greater than 1.75 mA could be used to distinguish non-bovine from other samples in a qualitative analysis. For quantitative analysis, bovine content was measured using the comparative ratio between copy number of bovine parathyroid and 12S rRNA genes. This ratio reflected the proportion of target bovine cells to total cell numbers. In the experiment, contents of bovine constituents in four kinds of tested pet foods were 10.88, 8.76, 6.39 and 2.69%. Compared with the first two samples on which defined content had been addressed, the estimated content with 90.66 and 87.60% accuracy could be obtained, respectively. Although, this quantitative detection was not a real-time determination, the method had several merits on its rapidity and simplicity in performing the test and on its cost effectiveness since no sophisticated devices and expensive reagents were needed. q

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Section: Methodsmentioning

confidence: 99%

Section: Quantitative Analysis Of Bovine Contents In Feedstuffsmentioning

confidence: 99%

Novel electrochemical identification and semi quantification of bovine constituents in feedstuffs

Chaumpluk

Chikae

Takamura

et al. 2006

Science and Technology of Advanced Materials

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“…It is worth noting that the CRE box is present in the promoters of several other genes known to be expressed in neuroendocrine cells, such as parathyroid hormone [24], proenkephalin A [25] and somatostatin [26]. Since these genes are inducible by CAMP [26], it is tempting to speculate that the expression of mouse CgB may be stimulated by those extracellular factors that increase intracellular CAMP levels.…”

Section: The Mouse Cgb Promotermentioning

confidence: 99%

The organisation of the mouse chromogranin B (secretogranin I) gene

Pohl¹,

Phillips²,

Song³

et al. 1990

FEBS Letters

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A cosmid clone containing the gene for mouse chromogranin B (secretogranin I), a secretory protein found in secretory granules of most endocrine cells and neurons, was isolated and sequenced. The chromogranin B protein was found to be encoded by 5 exons which correspond to the cleaved signal peptide, the short N-terminal sequence preceding the disulfide-bonded loop structure, the disulfide-bonded loop structure itself, the large, variable region comprising -90% of the protein, and the conserved C-terminal sequence. The promoter region of the chromogranin B gene is very GC-rich and contains a CATAA motif, a CAMP-responsive element and an Spl binding site.

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“…The coding region of amino acids 1 to 84 of mature PTH present on exon 3 of the bovine PTH gene (20) was amplified by polymerase chain reaction from genomic calf liver DNA using primers: 5Ј-GATCCTCAT-CATATGGCTGTGAGTGAAATACAGTTT-3Ј and 5Ј-GGTGGGATCCC-TATTACTGGGGTTTAGCTTTAATTAATA-3Ј. NdeI and BamHI restriction sites in the polymerase chain reaction primers were used to clone the amplified PTH gene fragment into vector pET-14B (Novagen, Madison, WI).…”

Section: Cloning and Purification Of Mature Bovine Parathyroid Hormone-mentioning

confidence: 99%

Megalin Antagonizes Activation of the Parathyroid Hormone Receptor

Hilpert¹,

Nykjaer²,

Jacobsen³

et al. 1999

Journal of Biological Chemistry

110

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Parathyroid hormone (PTH) is predominantly cleared from the circulation by glomerular filtration and degradation in the renal proximal tubules. Here, we demonstrate that megalin, a multifunctional endocytic receptor in the proximal tubular epithelium, mediates the uptake and degradation of PTH. Megalin was purified from kidney membranes as the major PTH-binding protein and shown in BIAcore analysis to specifically bind full-length PTH and amino-terminal PTH fragments (K d 0.5 M). Absence of the receptor in megalin knockout mice resulted in 4-fold increased levels of amino-terminal PTH fragments in the urine. In F9 cells expressing both megalin and the PTH/PTH-related peptide receptor (PTH/PTHrP receptor), uptake and lysosomal degradation of the hormone was mediated through megalin. Blocking megalin-mediated clearance of PTH resulted in 3-fold increased stimulation of the PTH/PTHrP receptor. These data provide evidence that megalin is involved in the renal catabolism of PTH and potentially antagonizes PTH/PTHrP receptor activity in the proximal tubular epithelium.

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Isolation and complete nucleotide sequence of the gene for bovine parathyroid hormone

Cited by 62 publications

References 20 publications

Novel electrochemical identification and semi quantification of bovine constituents in feedstuffs

Novel electrochemical identification and semi quantification of bovine constituents in feedstuffs

The organisation of the mouse chromogranin B (secretogranin I) gene

Megalin Antagonizes Activation of the Parathyroid Hormone Receptor

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