Isolation and complete genome sequence of a novel cyanophage, S-B05, infecting an estuarine Synechococcus strain: insights into environmental adaptation

Jiang, Yong; Guo, Cui; Wang, Min; Wang, Meiwen; You, Siyuan; Liu, Huan; Zhang, Xinran; Shao, Hongbing; McMinn, Andrew

doi:10.1007/s00705-020-04595-6

Cited by 9 publications

(11 citation statements)

References 51 publications

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“…The percolates were then filtered with a 50 kDa cartridge (Pellicon ® XL Cassette, Biomax ® 50 kDa; polyethersulfone, Millipore Corporation, Billerica, MA, USA) and concentrated through tangential flow to make the viral concentration reach 300 times the initial concentration. The viral concentrate was stored at 4 • C in the dark [26,27].…”

Section: Cyanophage Isolationmentioning

confidence: 99%

“…strain MW02 (NCBI accession number KP113680). The algal culture was grown in conical flasks with f/2 seawater medium under a constant illumination of approximately 25 µmol m −2 s −1 at 25 • C in a 12-h/12-h light-dark cycle [27]. The phage enrichment was performed by adding the viral-concentrated seawater to the exponentially growing host Synechococcus in a ratio of 1:9.…”

Section: Cyanophage Isolationmentioning

confidence: 99%

“…Phage purification was performed using the serial dilution method, as described previously [27]. Generally, the infectivity was tested across the serially diluted phage samples (10 times dilution over 7 orders of magnitude).…”

Section: Phage Purificationmentioning

confidence: 99%

“…The cyanophage was then concentrated using Amicon ® Ultra 15 with a 30 k-Da ultra-PL membrane (Merck, Ireland) [30]. Further purification was performed by sucrose density gradient centrifugation [27].…”

Section: Phage Purificationmentioning

confidence: 99%

“…Phage DNA was extracted from the sucrose density gradient-purified phages using a TIANamp Virus DNA Kit (TIANGEN) [27]. A total of 1 µg DNA per sample was used as input for the DNA sample preparations.…”

Section: Genome Sequencing and Assemblymentioning

confidence: 99%

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Genome Analysis of Two Novel Synechococcus Phages That Lack Common Auxiliary Metabolic Genes: Possible Reasons and Ecological Insights by Comparative Analysis of Cyanomyoviruses

Jiang

Guo

Wang

et al. 2020

Viruses

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The abundant and widespread unicellular cyanobacteria Synechococcus plays an important role in contributing to global phytoplankton primary production. In the present study, two novel cyanomyoviruses, S-N03 and S-H34 that infected Synechococcus MW02, were isolated from the coastal waters of the Yellow Sea. S-N03 contained a 167,069-bp genome comprising double-stranded DNA with a G + C content of 50.1%, 247 potential open reading frames and 1 tRNA; S-H34 contained a 167,040-bp genome with a G + C content of 50.1%, 246 potential open reading frames and 5 tRNAs. These two cyanophages contain fewer auxiliary metabolic genes (AMGs) than other previously isolated cyanophages. S-H34 in particular, is currently the only known cyanomyovirus that does not contain any AMGs related to photosynthesis. The absence of such common AMGs in S-N03 and S-H34, their distinct evolutionary history and ecological features imply that the energy for phage production might be obtained from other sources rather than being strictly dependent on the maintenance of photochemical ATP under high light. Phylogenetic analysis showed that the two isolated cyanophages clustered together and had a close relationship with two other cyanophages of low AMG content. Comparative genomic analysis, habitats and hosts across 81 representative cyanomyovirus showed that cyanomyovirus with less AMGs content all belonged to Synechococcus phages isolated from eutrophic waters. The relatively small genome size and high G + C content may also relate to the lower AMG content, as suggested by the significant correlation between the number of AMGs and G + C%. Therefore, the lower content of AMG in S-N03 and S-H34 might be a result of viral evolution that was likely shaped by habitat, host, and their genomic context. The genomic content of AMGs in cyanophages may have adaptive significance and provide clues to their evolution.

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Section: Cyanophage Isolationmentioning

confidence: 99%

Section: Cyanophage Isolationmentioning

confidence: 99%

Section: Phage Purificationmentioning

confidence: 99%

Section: Phage Purificationmentioning

confidence: 99%

Section: Genome Sequencing and Assemblymentioning

confidence: 99%

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