Isolation and Comparative Study on the Characterization of Guanidine Hydrochloride Soluble Collagen and Pepsin Soluble Collagen from the Body of Surf Clam Shell (Coelomactra antiquata)

Abstract

The aim of this study was to characterize the collagens from the body of surf clam shell (Coelomactra antiquata). Guanidine hydrochloride and pepsin were used to extract collagens. Guanidine hydrochloride soluble collagen (GSC) and pepsin soluble collagen (PSC) were separately isolated from the body of surf clam shell. Results showed that the moisture, protein, carbohydrate, and ash contents of the body of surf clam shell were 82.46%, 11.56%, 3.05%, and 2.38%, respectively, but the fat content was only 0.55%. The yields were 0.59% for GSC and 3.78% for PSC. Both GSC and PSC were composed of α1 and α2 chains and a β chain, however, GSC and PSC showed distinct differences from each other and the type I collagen from grass carp muscle on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). GSC and PSC contained glycine as the major amino acid and had imino acid of 150 and 155 residues/1000 residues, respectively. Fourier transform infrared spectroscopy (FTIR) spectra of GSC and PSC revealed the presence of a triple helix. The GSC appeared to have a dense sheet-like film linked by random-coiled filaments and PSC had fine globular filaments under scanning electron microscopy (SEM). The maximum transition temperature (Tmax) of GSC and PSC was 33.05 °C and 31.33 °C, respectively. These results provide valuable scientific information for the texture study and development of surf clam shell or other bivalve mollusks.

Fish Collagen: Extraction, Characterization, and Applications for Biomaterials Engineering

“…Type I collagen is found in the cartilage of Sphyrna lewini, Dasyatis akjei, and Raja porosa [ 59 ]. In Amur sturgeon ( Acipenser schrenckii ) cartilage, type I collagen was found in acid-solubilized and salt-solubilized collagen and type II with other minor types was found in pepsin-solubilized collagen [ 26 ]. Hoki cartilage-derived collagens have been reported to have similar α chain assembly, amino acid composition, and structure to collagens in mammalian cartilage; and they could have potential in biomaterials for the treatment of cartilage-related diseases [ 60 ].…”

“…The low amino acid content (proline and Hyp) in marine collagen causes the low denaturation temperature, which makes fish collagen challenging to handle as it denatures at human body temperature. At present, collagen from marine invertebrate organisms is under investigation, including jellyfish and marine sponges [ 24 , 25 , 26 ].…”

“…Collagen takes part in the construction of a fibrous network of cells called fibroblasts, which form the base for the growth of new cells. In areas such as the dermis, collagen plays an active role in the protection of the skin by inhibiting the absorption and spreading of pathogenic substances, environmental toxins, micro-organisms, and cancerous cells [ 2 , 26 ]…”

Utilization of marine by-products for the recovery of value-added products

“…Fish skin, scales, bone, and fins have been widely studied as alternative sources of collagen (Bae et al, 2008;Ehrlich et al, 2018;Hernández-Briones et al, 2009;Kim et al, 2016;Kim and Mendis 2006;Nomura, et al, 1995). In addition, it has been found that the mantle of scallops (Shen et al, 2007), the muscle layer of ascidians (Mizuta et al, 2002a), and the adductor of pearl oys- Shahidi et al Utilization of marine by-products for the recovery of value-added products ters (Mizuta et al, 2002b;Wu et al, 2019) can be used as new sources of collagen. Approximately, 36-54% of collagen could be obtained from fish skin, bone and fin (Nagai and Suzuki, 2000).…”

Utilization of marine by-products for the recovery of value-added products

“…Fish skin, scales, bone, and fins have been widely studied as alternative sources of collagen (Bae et al, 2008;Ehrlich et al, 2018;Hernández-Briones et al, 2009;Kim et al, 2016;Kim and Mendis 2006;Nomura, et al, 1995). In addition, it has been found that the mantle of scallops (Shen et al, 2007), the muscle layer of ascidians (Mizuta et al, 2002a), and the adductor of pearl oys- Shahidi et al Utilization of marine by-products for the recovery of value-added products ters (Mizuta et al, 2002b;Wu et al, 2019) can be used as new sources of collagen. Approximately, 36-54% of collagen could be obtained from fish skin, bone and fin (Nagai and Suzuki, 2000).…”