Isolation and Chemical Structural Characterisation of a Compound with Antioxidant Activity from the Roots of<i>Senna italica</i>

Mokgotho, M. P.; Gololo, Sechene Stanley; Masoko, Peter; Mdee, L. K.; Mbazima, Vusi; Shai, Jerry L.; Bagla, Victor Patrick; Eloff, Jacobus Nicolaas; Mampuru, L. J.

doi:10.1155/2013/519174

Cited by 25 publications

(17 citation statements)

References 18 publications

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“…These solid antioxidant activities might be attributed to its high total phenolic content as it found to be 474 mg gallic acid equivalent (GAE)/gm extract when estimated by the Folin-Ciocalteu method. Similar results were detected in extracts from Cassia abbreviata and other Senna species rich in proanthocyanidins [ 29 , 30 ].…”

Section: Resultssupporting

confidence: 81%

Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract

et al. 2017

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Natural products are considered as an important source for the discovery of new drugs to treat aging-related degenerative diseases and liver injury. The present study profiled the chemical constituents of a methanol extract from Senna singueana bark using HPLC-PDA-ESI-MS/MS and 36 secondary metabolites were identified. Proanthocyanidins dominated the extract. Monomers, dimers, trimers of (epi)catechin, (epi)gallocatechin, (epi)guibourtinidol, (ent)cassiaflavan, and (epi)afzelechin represented the major constituents. The extract demonstrated notable antioxidant activities in vitro: In DPPH (EC50 of 20.8 µg/mL), FRAP (18.16 mM FeSO4/mg extract) assays, and total phenolic content amounted 474 mg gallic acid equivalent (GAE)/g extract determined with the Folin-Ciocalteu method. Also, in an in vivo model, the extract increased the survival rate of Caenorhabditis elegans worms pretreated with the pro-oxidant juglone from 43 to 64%, decreased intracellular ROS inside the wild-type nematodes by 47.90%, and induced nuclear translocation of the transcription factor DAF-16 in the transgenic strain TJ356. Additionally, the extract showed a remarkable hepatoprotective activity against d-galactosamine (d-GalN) induced hepatic injury in rats. It significantly reduced elevated AST (aspartate aminotransferase), and total bilirubin. Moreover, the extract induced a strong cytoplasmic Bcl-2 expression indicating suppression of apoptosis. In conclusion, the bark extract of S. sengueana represents an interesting candidate for further research in antioxidants and liver protection.

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Section: Resultssupporting

confidence: 81%

Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract

et al. 2017

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“…According to [ 23 ] polyphenolic compound in plants may play a major role in the inhibition AGEs. Resveratrol which has been reported to be present in S. italica extract [ 24 ] might have played a role in the ability of the extract to inhibit protein glycation. Recent studies have also revealed that resveratrol has beneficial effects on the liver by extenuating oxidative stress and downregulation of receptor for AGE (RAGE) expression in the liver of rats with type 2 diabetes [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Potential Hypoglycaemic and Antiobesity Effects of Senna italica Leaf Acetone Extract

Malematja

Bagla

Njanje

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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Background Type II diabetes is on the rise while obesity is one of the strongest risk factors of type II diabetes. The search for a drug for type II that can equally mitigate obesity related complication is desired. Methods The acetone leaf extract of Senna italica was evaluated for its cytotoxic, antiglycation, and lipolytic effect, glucose uptake, and GLUT4 translocation and expression using published methods, while that for adipogenesis and protein expression levels of obesity related adipokines was assessed using adipogenesis assay and mouse adipokine proteome profiler kit, respectively. The possible mechanism of glucose uptake was assessed through the inhibition of PI3K pathway. Results The extract had no adverse effect on 3T3-L1 cell viability (CC50 > 1000 μg/ml). High antiglycation effect was attained at 10 mg/ml, while at 25–200 μg/ml it showed no significant increase in adipogenesis and lipolysis. The extract at 100 μg/ml was shown to decrease the expression levels of various adipokines and minimal glucose uptake at 50–100 μg/ml with a nonsignificant antagonistic effect when used in combination with insulin. GLUT4 translocation and expression were attained at 50–100 μg/ml with an increase in GLUT4 expression when in combination with insulin. Conclusion The acetone leaf extract of S. italica stimulates glucose uptake through the PI3K-dependent pathway and can serve as a source of therapeutic agent for the downregulation of obesity-associated adipokines in obesity and antiglycation agents.

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“…Calibrated plastic scale was used to measure the inhibition zones. The test experiment was repeated two times to ensure reliability 11,12 .…”

Section: Methodmentioning

confidence: 99%

Untitled

2017

IJPSR

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Eulophia nuda Lind. belongs to family Orichidaceae and is a rare and endangered orchid. Present research work was carried out on tuber extracts of Eulophia nuda for the evaluation of antimicrobial activities and hepatoprotective. Preliminary phytochemical screening revealed presence of phytochemical constituents like alkaloids, flavonoids, steroids, glycosides (cardiac), tannins, saponins, carbohydrates in three tuber extracts prepared by using solvents (chloroform, acetone and ethanol). Antibacterial activity was carried out with Disc Diffusion method against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Acetone extract was more effective against Staphylococcus aureus with maximum zone of inhibition 18 mm compared to standard antibiotic Ampicillin with zone of inhibition 20 mm. Antifungal studies was carried out using well diffusion method against Candida albicans, Aspergillus niger and Aspergillus flavus. Chloroform extract was more effective against Aspergillus niger having zone of inhibition 17 mm compared with standard antifungal Fluconazole (20mm). Acetone extract shown the zone of inhibition of 16 mm against Aspergillus flavus compared to standard Fluconazole (22mm). Hepatoprotective activity was carried out as per OECD guidelines 425 using Wistar albino rats. Effect of these extracts on CCl 4 induced hepatotoxic rats was studied by SGOT, SGPT and ALP parameters compared with standard LIV 52. From the research work, it was concluded that Eulophia nuda tuber extracts are active as antibacterial, antifungal and hepatoprotective which could be used for the development of some promising formulations, furthermore, structural elucidation of isolated components from the extracts of Eulophia nuda can be carried out using studies like IR and NMR.

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Isolation and Chemical Structural Characterisation of a Compound with Antioxidant Activity from the Roots ofSenna italica

Cited by 25 publications

References 18 publications

Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract

Senna singueana: Antioxidant, Hepatoprotective, Antiapoptotic Properties and Phytochemical Profiling of a Methanol Bark Extract

Potential Hypoglycaemic and Antiobesity Effects of Senna italica Leaf Acetone Extract

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