Isolation and Characterization of Zinc Solubilizing Bacteria  from Kashmir Himalayas

Jagana, Chanrda Sekhar; Baba, Zahoor Ahmad; Krishnanand, S.I.; Zargar, MY; Badri, Z.A.; Khan, Imran

doi:10.20546/ijcmas.2019.806.152

Cited by 3 publications

(3 citation statements)

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“…Manasa et al 25 isolated and screened Zn-solubilizing bacteria from plant rhizosphere in vitro, establishing the idea of rhizosphere colonization with these beneficial microorganisms. Similarly, the creation of a halo zone by different microorganisms in culture plates could be attributed to acidity transport during their active growth stages 6,13,26 ------------------------------------------------------------------------------------------------------ The frequency of isolated zinc solubilizers from tea rhizosphere soil in various mediums is shown in Table 1. The isolation frequency of ZSMs was more (3.0±1.4×10 5 CFU gG 1 dry soil-6.9±0.7×10 5 CFU gG 1 dry soil) when modified Bunt and Rovira medium was used as one of the selective media to isolate ZSMs from tea soil followed by modified PKV medium (1.0±0.9×10 5 CFU gG 1 dry soil-4.6±1.0×10 5 CFU gG 1 dry soil) and ZSA medium (1.0±0.9×10 5 CFU gG 1 dry soil-2.4±1.1×10 5 CFU gG 1 dry soil) irrespective of the bacteria or fungi under culture.…”

Section: Resultsmentioning

confidence: 99%

“…Variations in Zn solubilization potential among the isolates might be attributed to their isolation dynamics and variations in habitat or locations as well as their diverse abilities in solubilizing the insoluble source of mineral to its organic form. The formation of organic acids and consequent acidification of the medium could explain the drop in pH of the medium 13,20 . The most essential process for heterotrophic metal solubilization was recorded to be the production of H+ and organic acids 20 .…”

Section: Resultsmentioning

confidence: 99%

“…Rhizospheric bacteria can use a solubilization mechanism to convert an inaccessible metal into an available form 11 . Other studies isolated and screened zinc solubilizing microbes from various rhizosphere soils, establishing the potential of the rhizosphere soil environment as one of the richest niches harnessing the existence of Agriculturally Important Microbes (AIMs) like ZSMs that could be best utilised for accelerating zinc bioavailability to the growing plant 12,13 .…”

Section: Introductionmentioning

confidence: 99%

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Zinc-Solubilization Potential of Putative Microorganisms Isolated from Tea [Camellia sinensis (L.) O. Kuntze] Rhizosphere

Sarma,

Bhattacharya,

Sarmah

et al. 2022

Res. J. Microbiol

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Background and Objective: Monocropping farming practices in Northeastern tea soils are a major risk of zinc deficiency, leading to micronutrient malnutrition. Zinc Solubilizing Microorganisms (ZSMs) are important drivers in the solubilization of insoluble Zn compounds and consequently play an important role in soil fertility and nutrient allocation. The present study attempts to isolate, characterize and qualify the Zn-solubilizing microbes from the tea rhizosphere. Materials and Methods: Using a culture-dependent approach and dilution plate technique, the ZSMs were isolated and quantified in vitro from the tea rhizosphere. Standard methodologies were used to determine the effectiveness of Zn solubilization. Results: The 16 out of the 20 ZSM isolates were shown to have substantial halo zones. Bacteria accounted for 10 of the 16 ZSMs, whereas fungi for six. Modified Bunt and Rovira medium and 10 5 dilutions had the highest frequency of ZSM isolation (up to 6.9±0.7×10 5 CFU gG 1 dry soil), followed by modified Pikovskayas agar (4.6±1.0×10 5 CFU gG 1 dry soil). The Zn solubilisation index ranged from 125-325%. Several bacteria-produced acids, as evidenced by a drop in the pH of the broth medium. When given ZnO as the source of Zn, the strains produced Indole-3-Acetic Acid (IAA) (up to 12.5 ppm by ZSB#09). The most common fungus genera were Aspergillus spp. and Trichoderma spp. Gram-positive cocci were dominant among bacteria. Conclusion: As zinc deficiency is common in Northeastern tea soils, utilising the potential of ZSMs could lead to improve sustainability in Zn supplementation.

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