Isolation and Characterization of vB_MsmS_Celfi: A New<i>Mycobacterium tuberculosis</i>Bacteriophage

Payaslián, Florencia; Gradaschi, Victoria; Salazar, Liliana Rondón; Dieterle, M. Eugenia; Urdániz, Estefanía; Paola, Matilde de; Carcamo, J.; Zon, F. Van der; Sosa, Ezequiel; Porto, Darío Fernández Do; Dunne, Matthew; Goeller, Pauline; Klumpp, Jochen; Raya, Raúl R.; Reyes, Alejandro; Piuri, Mariana

doi:10.1089/phage.2020.0030

Cited by 2 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most show no infection, although Gabriela infects at a reduced EOP (10 23 ). This is consistent with the report that the subcluster L2 phage Celfi infects M. tuberculosis mc 2 6230, a derivative of M. tuberculosis H37Rv (45). The genomic basis for these differences is unclear, as subcluster L2 genomes are very closely related to each other (44).…”

Section: Resultssupporting

confidence: 90%

Toward a Phage Cocktail for Tuberculosis: Susceptibility and Tuberculocidal Action of Mycobacteriophages against Diverse Mycobacterium tuberculosis Strains

Bustamante

Dedrick

Garlena

et al. 2021

mBio

View full text Add to dashboard Cite

The global health burden of human tuberculosis (TB) and the widespread antibiotic resistance of its causative agent Mycobacterium tuberculosis warrant new strategies for TB control. The successful use of a bacteriophage cocktail to treat a Mycobacterium abscessus infection suggests that phages could play a role in tuberculosis therapy. To assemble a phage cocktail with optimal therapeutic potential for tuberculosis, we have explored mycobacteriophage diversity to identify phages that demonstrate tuberculocidal activity and determined the phage infection profiles for a diverse set of strains spanning the major lineages of human-adapted strains of the Mycobacterium tuberculosis complex. Using a combination of genome engineering and bacteriophage genetics, we have assembled a five-phage cocktail that minimizes the emergence of phage resistance and cross-resistance to multiple phages, and which efficiently kills the M. tuberculosis strains tested. Furthermore, these phages function without antagonizing antibiotic effectiveness, and infect both isoniazid-resistant and -sensitive strains. IMPORTANCE Tuberculosis kills 1.5 million people each year, and resistance to commonly used antibiotics contributes to treatment failures. The therapeutic potential of bacteriophages against Mycobacterium tuberculosis offers prospects for shortening antibiotic regimens, provides new tools for treating multiple drug-resistant (MDR)-TB and extensively drug-resistant (XDR)-TB infections, and protects newly developed antibiotics against rapidly emerging resistance to them. Identifying a suitable suite of phages active against diverse M. tuberculosis isolates circumvents many of the barriers to initiating clinical evaluation of phages as part of the arsenal of antituberculosis therapeutics.

show abstract

Section: Resultssupporting

confidence: 90%

Toward a Phage Cocktail for Tuberculosis: Susceptibility and Tuberculocidal Action of Mycobacteriophages against Diverse Mycobacterium tuberculosis Strains

Bustamante

Dedrick

Garlena

et al. 2021

mBio

View full text Add to dashboard Cite

show abstract

The CABANA model 2017–2022: research and training synergy to facilitate bioinformatics applications in Latin America

Campos-Sánchez,

Willis,

Gopalasingam

et al. 2024

Front. Educ.

View full text Add to dashboard Cite

The CABANA project (Capacity Building for Bioinformatics in Latin America) was funded by the UK’s Global Challenges Research Fund in 2017 with the aim to strengthen the bioinformatics capacity and extend its applications in Latin America focused on three challenge areas – communicable diseases, sustainable food production and protection of biodiversity. For 5 years, the project executed activities including data analysis workshops, train-the-trainer workshops, secondments, eLearning development, knowledge exchange meetings, and research projects in 10 countries. The project was successful in accomplishing all its goals with a major impact on the region. It became a model by which the research needs determined the training that was delivered. Multiple publications and over 800 trainees are part of the legacy of the project.

show abstract

Isolation and Characterization of vB_MsmS_Celfi: A NewMycobacterium tuberculosisBacteriophage

Cited by 2 publications

References 36 publications

Toward a Phage Cocktail for Tuberculosis: Susceptibility and Tuberculocidal Action of Mycobacteriophages against Diverse Mycobacterium tuberculosis Strains

Toward a Phage Cocktail for Tuberculosis: Susceptibility and Tuberculocidal Action of Mycobacteriophages against Diverse Mycobacterium tuberculosis Strains

The CABANA model 2017–2022: research and training synergy to facilitate bioinformatics applications in Latin America

Contact Info

Product

Resources

About