Isolation and characterization of urease-producing bacteria from tropical peat

Phang, Ignatius Ren Kai; Chan, Yen San; Wong, Kwong Soon; Lau, Sie Yon

doi:10.1016/j.bcab.2017.12.006

Cited by 48 publications

(35 citation statements)

References 72 publications

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“…Different soil bacteria has different tolerance towards pH conditions. Some ureolytic bacteria including Bacillus cereus show higher activity at around pH 4 compared to the neutral pH conditions suggesting acidic urease compatibility [44]. Also, the bacteria (for example Helicobacter pylori) living in harsh acidic environment produce more urease enzyme at acidic conditions [45].…”

Section: Discussionmentioning

confidence: 99%

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Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

et al. 2019

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Microbial induced carbonate precipitation (MICP) is relatively an innovative soil improvement technique, learnt from the bio-mediated geochemical reactions that naturally occur in the earth surface. During the MICP, CaCO 3 is metabolically precipitated in soil pores, cement the particle contacts and improves the strength and stiffness of soil. Environment temperature is one of the most key factors that determines the efficiency MICP. The purpose of this study is to investigate the feasibility of stabilizing the slope soil of cold subarctic region (Hokkaido, Japan). The implication of MICP in cold subarctic zones remains as a major challenge, as the enzymatic performance of the bacteria typically declines during lower temperatures hence insufficient formation of CaCO 3 in soil matrix. Therefore, as a potential approach, this study attempted to investigate the feasibility of using the bacteria which have been adapted to native cold climatic conditions. The objectives of this paper are evaluating (1) the effect of temperature in bacterial response, and (2) the effect of grain size distribution in cementation mechanism. The observations suggest that the enzyme activity of the bacteria is negligible at and above 30 °C, whereas it is significant at relatively lower temperatures. The comparison of treated soils suggests that the fine content in slope soil increased number of particle contacts, facilitated effective packing, and promoted the effectiveness of MICP compared to that of uniformly graded sands. Finally, the technical feasibility in slope soil stabilization was well demonstrated using model solidification test. The limitations in stabilizing the slope are also discussed in detail.

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Section: Discussionmentioning

confidence: 99%

“…Similar phenomena could be seen on certain microbial urease like Sporosarcina pasteurii [23,40], Pararhodobacter sp. [27] and Klebsiella aerogenes [44], those have the optimum activity under weak alkaline conditions.…”

Section: Discussionmentioning

confidence: 99%

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

et al. 2019

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“…Investigations on use of ureolysis-driven MICP process has been widely studied for the soil strengthening and stabilization, remediation of concrete cracks, restoration of limestone surfaces, mitigation of soil erosion, treatment of industrial wastewater and remove heavy metals (Burbank et al, 2013;De Muynck et al, 2010;Fu and Wang, 2011;Işik et al, 2010;Sarda et al, 2009;Van Tittelboom et al, 2010;Whiffin et al, 2007). MICP via ureolysis process can also be used for biosensor, agriculture and healthcare applications (Phang et al, 2018). Nonetheless, MICP is frequently employed to produce bio-calcifying agent (CaCO 3 ) which is used as a construction and building material to improve the geotechnical properties of soils.…”

Section: Introductionmentioning

confidence: 99%

Low-cost cultivation of Sporosarcina pasteurii strain in food-grade yeast extract medium for microbially induced carbonate precipitation (MICP) application

Omoregie

Ngu

Ong

et al. 2019

Biocatalysis and Agricultural Biotechnology

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“…There was an increase in urease activity with an increase of pH from 3.0-9.0 (Khan et al 2019). Some bacterial ureases exhibited high activity in alkaline conditions (pH of 9.0) (Phang et al 2018), while some had maximum urease activity at pH 8 (Danial et al 2015;Mirbod et al 2002). Two fungal isolates of the genus Aspergillus had an optimum pH of 8.0 and 8.5 (Kappaun et al 2018).…”

Section: Purification and Characterization Of Uamentioning

confidence: 97%

Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

Nathan

Jasna

Parvathi

2020

Mar Life Sci Technol

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Mangrove ecosystems are one of the most versatile habitats for microorganisms with a high potential for producing a variety of extracellular hydrolytic enzymes. In this study, bacteria with urease activity, enzymes that catalyze the hydrolysis of urea into carbon dioxide and ammonia, were isolated from mangrove sediments of Poovar (Trivandrum, India). Bacillus halodurans, strain PO15, isolated in this study with high urease (UA) activity (28 U/ml) was subjected to optimization using a Box-Behnken experimental design. Incubation variables included incubation period, pH, inoculation percentage and temperature. Significant factors identified based on the model were incubation period, pH, incubation temperature, and inoculum percentage; variations in these produced a tenfold increase in UA activity (295.80 U/ml). The specific activity of the purified UA enzyme was 62.34 U/mg and was found to be thermostable (active up to 60 °C). UA of B. halodurans PO15 has potential for microbial-induced biomineralization with a reduction of free Ca 2+ to about 82.8% ± 0.17%. The microbialinduced calcium precipitation (MICP) using the UA enzyme will potentially be beneficial in the process of biomineralization as well as for a variety of industrial applications.

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Isolation and characterization of urease-producing bacteria from tropical peat

Cited by 48 publications

References 72 publications

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

Feasibility study for slope soil stabilization by microbial induced carbonate precipitation (MICP) using indigenous bacteria isolated from cold subarctic region

Low-cost cultivation of Sporosarcina pasteurii strain in food-grade yeast extract medium for microbially induced carbonate precipitation (MICP) application

Optimization of urease production by Bacillus halodurans PO15: a mangrove bacterium from Poovar mangroves, India

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