Isolation and characterization of two new Drosophila protein kinase C genes, including one specifically expressed in photoreceptor cells

Schaeffer, Eric; Smith, Dean P.; Mardon, Graeme; Quinn, William G.; Zuker, Charles S.

doi:10.1016/0092-8674(89)90915-x

Cited by 162 publications

(97 citation statements)

References 55 publications

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“…Members of the PKC family of serine/threonine protein kinases are activated by Ca 2+ and DAG, and an eye-enriched PKC encoded by the inaC (inactivation nor afterpotential C) locus [162] is localized to the rhabdomeres and is required for deactivation of the visual cascade [2,163]. Ca 2+ influx through the TRP channels is much higher in the inaC mutant than in the wild-type, indicating that INAC is required for inactivation of the TRP channels [161].…”

Section: Ca 2+ -Dependent Termination Of the Photoresponsementioning

confidence: 99%

Phototransduction and retinal degeneration in Drosophila

Wang

Montell

2007

Pflugers Arch - Eur J Physiol

259

303

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Drosophila visual transduction is the fastest known G-protein-coupled signaling cascade and has therefore served as a genetically tractable animal model for characterizing rapid responses to sensory stimulation. Mutations in over 30 genes have been identified, which affect activation, adaptation, or termination of the photoresponse. Based on analyses of these genes, a model for phototransduction has emerged, which involves phosphoinoside signaling and culminates with opening of the TRP and TRPL cation channels. Many of the proteins that function in phototransduction are coupled to the PDZ containing scaffold protein INAD and form a supramolecular signaling complex, the signalplex. Arrestin, TRPL, and Gα q undergo dynamic light-dependent trafficking, and these movements function in long-term adaptation. Other proteins play important roles either in the formation or maturation of rhodopsin, or in regeneration of phosphatidylinositol 4,5-bisphosphate (PIP 2 ), which is required for the photoresponse. Mutation of nearly any gene that functions in the photoresponse results in retinal degeneration. The underlying bases of photoreceptor cell death are diverse and involve mechanisms such as excessive endocytosis of rhodopsin due to stable rhodopsin/arrestin complexes and abnormally low or high levels of Ca 2+ . Drosophila visual transduction appears to have particular relevance to the cascade in the intrinsically photosensitive retinal ganglion cells in mammals, as the photoresponse in these latter cells appears to operate through a remarkably similar mechanism.

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Section: Ca 2+ -Dependent Termination Of the Photoresponsementioning

confidence: 99%

Phototransduction and retinal degeneration in Drosophila

Wang

Montell

2007

Pflugers Arch - Eur J Physiol

259

303

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“…It was coupled to BSA and used to produce antisera. Eleven of the 15 residues in this sequence are also conserved in yeast SNF1, whereas the second best match-up identified in a search of the SwissProt database was found in PKC2, a protein kinase C homolog from Drosophila (Schaeffer et al, 1989), in which only five of the residues are conserved (Fig. 4).…”

Section: Synthesis Of a Peptide With A Sequence Characteristic Of Plamentioning

confidence: 99%

Evidence That Barley 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Kinase Is a Member of the Sucrose Nonfermenting-1-Related Protein Kinase Family

et al. 1996

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A protein kinase was partially purified from barley (Hordeum vulgare L. cv Sundance) endosperm by ammonium sulfate fractionation, followed by ion-exchange, Reactive Blue, Mono-Q, and phosphocellulose chromatography. It was shown to phosphorylate Arabidopsis 3-hydroxy-3-methylglutaryl-coenzyme A (HMC-COA) reductase and a synthetic peptide that was shown previously to act as a substrate for HMC-COA reductase kinase purified from cauliflower, confirming it to be barley HMG-COA reductase kinase. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the partially purified preparation showed the presence of a polypeptide with an approximate relative molecular weight (M,) of 60,000, which is the size predicted for the barley sucrose nonfermenting-1 (SNF1)-related protein kinases BKIN2 and BKIN12. Antisera were raised to a rye (Secale cereale 1.) SNF1-related protein kinase (RKIN1) expressed in Fscherichia coli as a fusion with maltosebinding protein and to a synthetic peptide with a sequence that is conserved in, and specific to, plant members of the SNF1-related protein kinase family. l h e maltose-binding protein-RKIN1 fusion protein antiserum recognized a doublet of polypeptides with an approximate M, of 60,000 in crude endosperm extracts and a single polypeptide in root extracts, which co-migrated with the smaller polypeptide in the endosperm doublet. Both antisera recognized a polypeptide with an approximate M, of 60,000 i n the partially purified protein kinase preparation, suggesting strongly that barley HMC-COA reductase kinase is a member of the SNFl-related protein kinase family.

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“…INAC has been shown to play critical roles in deactivating TRP channels [61]. INAC is one member of the PKC family of serine/threonine protein kinases [62,63], which is activated by Ca 2+ and DAG [64]. Using phosphorylation assays in isolated signaling complexes, TRP was identified to undergo Ca 2+ -dependent phosphorylation modification [61].…”

Section: Deactivation Of the Phototransduction Cascadementioning

confidence: 99%

Phototransduction in Drosophila

Tian

Tong

et al. 2012

Sci. China Life Sci.

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The Drosophila visual transduction is the fastest known G protein-coupled signaling cascade and has been served as a model for understanding the molecular mechanisms of other G protein-coupled signaling cascades. Numbers of components in visual transduction machinery have been identified. Based on the functional characterization of these genes, a model for Drosophila phototransduction has been outlined, including rhodopsin activation, phosphoinoside signaling, and the opening of TRP and TRPL channels. Recently, the characterization of mutants, showing slow termination, revealed the physiological significance and the mechanism of rapid termination of light response.

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Isolation and characterization of two new Drosophila protein kinase C genes, including one specifically expressed in photoreceptor cells

Cited by 162 publications

References 55 publications

Phototransduction and retinal degeneration in Drosophila

Phototransduction and retinal degeneration in Drosophila

Evidence That Barley 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Kinase Is a Member of the Sucrose Nonfermenting-1-Related Protein Kinase Family

Phototransduction in Drosophila

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