Abstract:Summary. Prolactin from frozen turkey pituitary glands was purified by alkaline ethanol extraction of the pituitary residue after pre-extractions of the gonadotrophins and GH, followed by gel filtration on Sephadex G-100 and DEAE\p=m-\cellulosecolumn chromatography at different pH. A specific radioligand\p=m-\receptorassay for ovine prolactin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified turkey prolactin was characterized by a radi… Show more
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