ISOLATION AND CHARACTERIZATION OF TRYPSIN INHIBITOR FROM THE WATER FERN, AZOLLA PINNATA R.Br.

Maity, Joydev; Patra, Bidhan Chandra

doi:10.1111/j.1745-4514.2003.tb00283.x

Cited by 5 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kunitz inhibitors are stored in a number of plant tissues, including seeds, tubers, ,, leaves, rhizome, , fruits, reproductive organs, and leaves. , It is known that the process of subexpression and the location of these inhibitors in certain tissues depend on the genes that code for the production of these proteins; they may be expressed constitutively or as inducible plant defense genes . According to Speransky and co-workers, Kunitz-type protease inhibitors (KPIs) are induced during pathogen infection, suggesting that these inhibitors play a key role in defense against lytic enzymes involved in insect and pathogen attacks.…”

mentioning

confidence: 99%

“…12 However, Kunitz inhibitors are also able to inhibit other proteinases, such as aspartic and cysteine proteinases. 13,14 Kunitz inhibitors are stored in a number of plant tissues, including seeds, 15 tubers, 5,16,17 leaves, 18 rhizome, 19,20 fruits, 21 reproductive organs, 22 and leaves. 23,24 It is known that the process of subexpression and the location of these inhibitors in certain tissues depend on the genes that code for the production of these proteins; they may be expressed constitutively or as inducible plant defense genes.…”

mentioning

confidence: 99%

See 1 more Smart Citation

A Kunitz Proteinase Inhibitor from Corms of Xanthosoma blandum with Bactericidal Activity

et al. 2011

View full text Add to dashboard Cite

Bacterial infections directly affect the world's population, and this situation has been aggravated by indiscriminate use of antimicrobial agents, which can generate resistant microorganisms. In this report, an initial screening of proteins with antibacterial activity from corms of 15 species of the Xanthosoma genus was conducted. Since Xanthosoma blandum corms showed enhanced activity toward bacteria, a novel protein with bactericidal activity was isolated from this particular species. Edman degradation was used for protein N-termini determination; the primary structure showed similarities with Kunitz inhibitors, and this protein was named Xb-KTI. This protein was further challenged against serine proteinases from different sources, showing clear inhibitory activities. Otherwise, no hemolytic activity was observed for Xb-KTI. The results demonstrate the biotechnological potential of Xb-KTI, the first proteinase inhibitor with antimicrobial activity described in the Xanthosoma genus.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

A Kunitz Proteinase Inhibitor from Corms of Xanthosoma blandum with Bactericidal Activity

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Antinutritional factors are inescapable in most plants intended for human consumption such as cereals and legumes [50,51]. In the case of Azolla species, these factors include phytic acid, cyanide [52] and tannins that are associated with proteins, which difficult its application as supplement [53] due to potential inhibitions on the digestive enzyme trypsin [54].…”

Section: Azolla and Its Importance For The Agri-food Sectormentioning

confidence: 99%

Agricultural and Forestry Importance of Microorganism-plant Symbioses: A Microbial Source for Biotechnological Innovations

Carballo-Sánchez

Alarcón

Pérez-Moreno

et al. 2022

RAS

View full text Add to dashboard Cite

The rhizosphere is the soil that surrounds, and is influenced by, the roots of a plant. It is considered one of the most complex ecosystems on the planet due to the intense interactions that occur between plants and microorganisms, as well as the competition that occurs among the microbial components. Due to this competition and beneficial interactions, a contribution of paramount relevance occurs in terms of chemical, physical, and biological characteristics that allow the plant and crop development. To mitigate ecosystem disturbances, it is necessary to compensate the imbalance of these conditions. Unfortunately, human activities involving strong soil disturbance have significantly affected plant development. Therefore, currently it is a priority to avoid the deterioration of soil ecosystems to remediate the damages that have already occurred. In the case of soil microbiology area, there are many solutions that can be designed and applied with beneficial microorganisms, including both fungi and bacteria, that interact mutualistically with plants or crops. At the same time, a deep understanding of these interactions remains challenging due to their complexity. In this review, biotechnological developments with agricultural or forestry importance are analysed. These include plant growth promoter bacteria, the Azolla-Anabaena symbiotic system, arbuscular mycorrhizal fungi and ectomycorrhizal fungi, as well as their relevance in the production of agricultural and forestry biofertilizers.

show abstract

“…Isolating and identifying the T1 from Azolla leaves has not been attempted very often. Trypsin inhibitor from water fern was isolated and characterized by Maity and Patra (2003).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Micro-nutritional and Anti-nutritional Aspects of Azolla for its Application as Functional Food Ingredient

Chandrababu,

Parvathy,

Meenu

et al. 2024

AJDFR

View full text Add to dashboard Cite

Background: Aquatic plants are unexploited sources of bioactive and functional compounds having the potential as food ingredients for developing novel functional food and nutraceutical products. Azolla, an aquatic fern, is an excellent source of nutrients and secondary metabolites and hence finds application possibilities in the human diet. The goal of the current investigation was to expore the micronutrients as well as identify certain anti-nutritional elements in the water fern Azolla. Methods: In two species of Azolla viz., Azolla pinnata and Azolla caroliniana; the micro and anti-nutritional factors were determined using standard laboratory methods. Result: The findings demonstrated that both species of Azolla contained hydrocyanide, phytic acid, oxalate, tannin and saponin, all of which were below the critical level. Comparatively, saponin was found to be in the highest concentration of 9.13±0.012% and 7.52±0.031% and hydrocyanide was the lowest with the content of 0.0045±0.0004% and 0.0036±0.0002% for A. pinnata and A. caroliniana, respectively. Azolla caroliniana, however, outperformed Azolla pinnata in total caroteniod concentration, demonstrating its superior cardio-protective and anti-cancerous properties. Results indicated Azolla pinnata species to be more suitable for fortification as it contained lower anti-nutrients and was high in chlorophyll content when compared to the counterpart.

show abstract

ISOLATION AND CHARACTERIZATION OF TRYPSIN INHIBITOR FROM THE WATER FERN, AZOLLA PINNATA R.Br.

Cited by 5 publications

References 35 publications

A Kunitz Proteinase Inhibitor from Corms of Xanthosoma blandum with Bactericidal Activity

A Kunitz Proteinase Inhibitor from Corms of Xanthosoma blandum with Bactericidal Activity

Agricultural and Forestry Importance of Microorganism-plant Symbioses: A Microbial Source for Biotechnological Innovations

Exploring the Micro-nutritional and Anti-nutritional Aspects of Azolla for its Application as Functional Food Ingredient

Contact Info

Product

Resources

About