Isolation and characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos

Suasnavas, Edison; Heywood, Sierra; Ward, Anika; Cox, Lindsay; O'Grady, Merecedes; Zhao, Yuanfeng; Gilbert, Lacey; Isom, S. Clay

doi:10.1016/j.anireprosci.2015.01.012

Cited by 9 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the main problem of most CASA-Morph studies so far is the use of a low sample size 232688899091. Only a few authors24929394 have conducted large-scale studies to assess, in the same species, the sperm head morphometry and also to study its relationship with sperm function.…”

Section: Assessment Of Sperm Morphometry I – Technological Aspectsmentioning

confidence: 99%

“…Most of the research made in this aspect has usually been conducted at an interspecific level44101 since finding differences between species is easier than intraspecific or intra-male levels. Studies at the intraspecific level are quite limited and most of them have used a low sample size (fewer than 25 individuals)23888991 making robust conclusions difficult to obtain.…”

Section: Assessment Of Sperm Morphometry II – Functional Aspectsmentioning

confidence: 99%

See 1 more Smart Citation

Current status and potential of morphometric sperm analysis

et al. 2016

View full text Add to dashboard Cite

The spermatozoon is the most diverse cell type known and this diversity is considered to reflect differences in sperm function. How the diversity in sperm morphology arose during speciation and what role the different specializations play in sperm function, however, remain incompletely characterized. This work reviews the hypotheses proposed to explain sperm morphological evolution, with a focus on some aspects of sperm morphometric evaluation; the ability of morphometrics to predict sperm cryoresistance and male fertility is also discussed. For this, the evaluation of patterns of change of sperm head morphometry throughout a process, instead of the study of the morphometric characteristics of the sperm head at different stages, allows a better identification of the males with different sperm cryoconservation ability. These new approaches, together with more studies employing a greater number of individuals, are needed to obtain novel results concerning the role of sperm morphometry on sperm function. Future studies should aim at understanding the causes of sperm design diversity and the mechanisms that generate them, giving increased attention to other sperm structures besides the sperm head. The implementation of scientific and technological advances could benefit the simultaneous examination of sperm phenotype and sperm function, demonstrating that sperm morphometry could be a useful tool for sperm assessment.

show abstract

Section: Assessment Of Sperm Morphometry I – Technological Aspectsmentioning

confidence: 99%

Section: Assessment Of Sperm Morphometry II – Functional Aspectsmentioning

confidence: 99%

Current status and potential of morphometric sperm analysis

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Thus, a narrow range of SOX2 expression is needed to prevent differentiation (Kopp et al, ). Indeed, cessation of SOX2 expression is observed in the in vivo‐derived trophectdoderm of pre‐implantation porcine blastocysts compared to the epiblast and hypoblast tissue (du Puy et al, ; Fujii et al, ; Gao et al, ), while porcine trophectoderm stem cell lines displayed no SOX2 expression (Suasnavas et al, ). Similarly, SOX2 expression is down‐regulated in the trophectoderm of elongating bovine blastocysts (Degrelle et al, ).…”

Section: Discussionmentioning

confidence: 99%

Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

et al. 2017

View full text Add to dashboard Cite

Thirteen independent induced bovine trophectroderm (iBT) cell lines were established by reprogramming bovine fetal liver-derived fibroblasts after viral-vector transduction with either six or eight factors, including POU5F1 (OCT4), KLF4, SOX2, MYC, NANOG, LIN28, SV40 large T antigen, and hTERT. Light- and electron-microscopy analysis showed that the iBT cells had epithelial cell morphology typical of bovine trophectoderm cells. Reverse-transcription-PCR assays indicated that all of the cell lines expressed interferon-tau (IFNT) at passages 1 or 2. At later passages (≥ passage 8), however, immunoblot and antiviral activity assays revealed that more than half of the iBT cell lines had stopped expressing IFNT. Messenger RNAs specific to trophectoderm differentiation and function were found in the iBT cell lines, and 2-dimensional-gel analysis for cellular proteins showed an expression pattern similar to that of trophectoderm cell lines derived from bovine blastocysts. Integration of some of the human reprogramming factors, including POU5F1, KLF4, SOX2, MYC, NANOG, and LIN28, were detected by PCR, but their transcription was mostly absent in the iBT cell lines. Gene expression assessment of endogenous bovine reprogramming factor orthologs revealed endogenous bLIN28 and bMYC transcripts in all; bSOX2 and bNANOG in none; and bKLF4 and bPOU5F1 in less than half of the iBT cell lines. These results demonstrate that bovine trophectoderm can be induced via reprogramming factor expression from bovine liver-derived fibroblasts, although other fibroblast populations-e.g., derived from fetal thigh tissue-may produce similar results, albeit at lower frequencies.

show abstract

“…As previously described by McGill et al (2016) , RNA was isolated from muscle samples using Trizol Reagent (Life Technologies, Carlsbad, CA, USA), and, after RNA isolation, cDNA was created using Superscript III Reverse Transcriptase and random primers (Invitrogen, Waltham, MA, USA) following the manufacturer’s instructions. Gene expression analysis was performed using a 96.96 Dynamic Array Integrated Fluidic Circuit (Fluidigm, San Francisco, CA, USA) as previously described by Suasnavas et al (2015) . Supplemental Table S1 lists the 24 targeted genes used for analysis.…”

Section: Methodsmentioning

confidence: 99%

Effect of increasing zinc supplementation on post-transit performance, behavior, blood and muscle metabolites, and gene expression in growing beef feedlot steers

Heiderscheit

Hansen

2022

Journal of Animal Science

View full text Add to dashboard Cite

Fifty-four Angus-cross steers (297 kg ± 12) were stratified by body weight (BW) to pens (6 steers per pen) to determine effects of supplemental Zn on post-transit growth performance and blood and muscle metabolites. Dietary treatments started 25 d before trucking: control (CON; analyzed 54 mg Zn/kg DM), industry (IND; CON + 70 mg supplemental Zn/kg DM), and supranutritional Zn (SUPZN; CON + 120 mg supplemental Zn/kg DM). Supplemental Zn was bis-glycinate bound Zn (Plexomin Zn; Phytobiotics North America, Cary, NC). On d 0, steers were loaded onto a commercial trailer and transported 18 h (1,822 km). Individual BW were recorded on d -26, -25, -1, 0 (pre-transit), 1 (post-transit), 6, 27, and 28. Blood was collected on d -1, 1, 6, and 27. Longissimus thoracis biopsies were collected on d -1, 1, and 28. Daily individual feed disappearance was recorded via GrowSafe bunks. Data were analyzed using Proc Mixed of SAS with fixed effect of diet and steer as the experimental unit (growth performance, blood: n = 18 steers per treatment; muscle: n = 12 steers per treatment). Individual initial BW was used as a covariate in BW analysis. Contrast statements to test linear, quadratic, and Zn effects were used to analyze performance and blood parameters. Repeated measures analysis was used for post-transit DMI recovery and weekly post-transit DMI and Zn intake with the repeated effect of time. MetaboAnalyst 5.0 was utilized for statistical analysis of d 1 (off truck) muscle metabolites. Plasma Zn linearly increased due to Zn on d 1, 6, and 27 (P = 0.01), and off-truck (d 1) serum lactate increased over d -1 by 20, 0, and 20% in CON, IND, and SUPZN, respectively (Quadratic: P = 0.01). Muscle lactate tended to increase post-transit in CON and IND (P ≤ 0.07) but not SUPZN. Muscle metabolites relating to amino acid and nitrogen metabolism were increased in all treatments post-transit (P ≤ 0.02), and alanine-glucose cycle metabolites tended to increase in CON and IND (P ≤ 0.07). Steers supplemented with Zn recovered pre-transit DMI quicker than CON (by d 2: P = 0.01), while IND had greater overall post-transit DMI than CON with SUPZN intermediate (P = 0.04), and Zn-fed steers had greater ADG post-transit (P = 0.04). Zinc supplementation mitigated muscle or serum lactate increases due to transit and increased post-transit ADG.

show abstract

Isolation and characterization of trophoblast-derived stem-like cells from peri-implantation porcine embryos

Cited by 9 publications

References 35 publications

Current status and potential of morphometric sperm analysis

Current status and potential of morphometric sperm analysis

Bovine trophectoderm cells induced from bovine fibroblasts with induced pluripotent stem cell reprogramming factors

Effect of increasing zinc supplementation on post-transit performance, behavior, blood and muscle metabolites, and gene expression in growing beef feedlot steers

Contact Info

Product

Resources

About