Isolation and characterization of trimeric and monomeric PSI cores from Acaryochloris marina MBIC11017

Nagao, Ryo; Ogawa, Hiroto; Tsuboshita, Naoki; Kato, Kuniki; Toyofuku, Reona; Tomo, Tatsuya; Shen, Jian‐Ren

doi:10.1007/s11120-023-01025-x

Cited by 7 publications

(3 citation statements)

References 48 publications

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“…3A) and characterized fluorescence properties of specific fractions. Fraction F3 primarily contained PSI complexes based on position in the density gradient and similarity of pigment absorption and emission compared with published results for iron-replete MBIC11017 (37,49,50). Notably, the F3 absorption spectrum for SW strain MBIC11017 is missing a prominent longer-wavelength shoulder that is observed in MU05 and P4 (Fig.…”

Section: Possible Sources Of Functional Variation Among a Marina Spec...supporting

confidence: 66%

Niche partitioning in a cyanobacterium through divergence of its novel chlorophylld-based light-harvesting system

Ulrich,

Shen,

Bryant

et al. 2024

Preprint

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The evolution of novel traits can have important consequences for biological diversification. New ecological opportunities provided by a novel trait can trigger subsequent trait modification or niche partitioning; however, the underlying mechanisms of novel trait diversification are still poorly understood. Here, we report that the innovation of a new chlorophyll (Chl) pigment, Chld, by the cyanobacteriumAcaryochloris marinawas followed by the functional divergence of its light-harvesting complex. We identified three major photosynthetic spectral types based on Chl fluorescence properties forA. marinalaboratory strains, with shorter and longer wavelength types more recently derived from an ancestral intermediate phenotype. Members of the different spectral types exhibited extensive variation in the Chl-binding proteins as well as the Chl energy levels of their photosynthetic complexes. This spectral type divergence is associated with differences in the wavelength dependence of both growth rate and photosynthetic oxygen evolution. We conclude that the divergence of the light-harvesting apparatus has consequently impactedA. marinaecological diversification through specialization on different far-red photons for photosynthesis.

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Section: Possible Sources Of Functional Variation Among a Marina Spec...supporting

confidence: 66%

Niche partitioning in a cyanobacterium through divergence of its novel chlorophylld-based light-harvesting system

Ulrich,

Shen,

Bryant

et al. 2024

Preprint

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“…The abundance of these two bacterial genera and their associated functions may contribute to the sponge's biological fitness. Acaryochloris MBIC11017 was more abundant in green sponges (the top 20 genera) than in yellow sponges and may contribute to the green colouration since it contains chlorophylls a and d (Miyake et al 2020;Nagao et al 2023). Likewise, the higher abundance of cyanobacterial taxa in green sponges (Supplementary information, Fig.…”

Section: Discussionmentioning

confidence: 98%

Does the microbial community play a role in the different colour morphs of the breadcrumb sponge Halichondria panicea?

Alhaddad,

Caldwell,

Clare

2024

Preprint

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Halichondria panicea has two different seasonal colours, green and yellow, which may reflect the effect of temperature on the abundance of green microalgal species. There is, however, no definitive identification of the strain responsible for the colour change. This question was addressed by metagenomic analysis of the 16S and 18S rRNA V4 regions and comparison of chlorophyll fluorescence of the two colour morphs by epifluorescence microscopy. H. panicea samples were collected for analysis in four seasons in 2022 along the Northumberland coast, North East England. The chlorophyll concentration in green sponges was higher than in yellow sponges. Moreover, the bacterial and cyanobacterial abundance, diversity, and predicted bacterial functions were significantly higher in the green morph. The eukaryotic community in green sponges contained an abundance of specific green microalgal genera and higher eukaryotic abundance. Interestingly, yellow sponges had a higher abundance of golden-brown dinoflagellates known as the Dino-Group-I-Clade-I genus. These dinoflagellates exhibit parasitic behaviour in marine organisms. It is suggested, therefore, that the yellow colour is a sign of a diseased H. panicea rather than a seasonal colour morph. A comprehensive analysis of 16S and 18S rRNA sequences from sponge samples and their predicted functions provided a deeper understanding of sponge colour variations.

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“…Recent structural analyses have also contributed to theoretical studies on the physical processes occurring within type-I RCs. ,,,,− Adolphs et al calculated the excitonic coupling strength between every pair of Chl a molecules on PSI using the Poisson TrESP (P-TrESP) method and obtained the spatial distribution of the site energy shift (SES) values using the charge density coupling (CDC) method. SES values indicate the differences in the electrostatic interactions between the excited and ground states of the Chl a molecules and the surrounding polypeptides.…”

Section: Introductionmentioning

confidence: 99%

Experimental and Theoretical Mutation of Exciton States on the Smallest Type-I Photosynthetic Reaction Center Complex of a Green Sulfur Bacterium Chlorobaclum tepidum

Kimura,

Kitoh-Nishioka,

Kondo

et al. 2024

J. Phys. Chem. B

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The exciton states on the smallest type-I photosynthetic reaction center complex of a green sulfur bacterium Chlorobaculum tepidum (GsbRC) consisting of 26 bacteriochlorophylls a (BChl a) and four chlorophylls a (Chl a) located on the homodimer of two PscA reaction center polypeptides were investigated. This analysis involved the study of exciton states through a combination of theoretical modeling and the genetic removal of BChl a pigments at eight sites. ( 1) A theoretical model of the pigment assembly exciton state on GsbRC was constructed using Poisson TrESP (P-TrESP) and charge density coupling (CDC) methods based on structural information. The model reproduced the experimentally obtained absorption spectrum, circular dichroism spectrum, and excitation transfer dynamics, as well as explained the effects of mutation. (2) Eight BChl a molecules at different locations on the GsbRC were selectively removed by genetic exchange of the His residue, which ligates the central Mg atom of BChl a, with the Leu residue on either one or two PscAs in the RC. His locations are conserved among all type-I RC plant polypeptide, cyanobacteria, and bacteria amino acid sequences. (3) Purified mutant-GsbRCs demonstrated distinct absorption and fluorescence spectra at 77 K, which were different from each other, suggesting successful pigment removal. (4) The same mutations were applied to the constructed theoretical model to analyze the outcomes of these mutations. (5) The combination of theoretical predictions and experimental mutations based on structural information is a new tool for studying the function and evolution of photosynthetic reaction centers.

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Isolation and characterization of trimeric and monomeric PSI cores from Acaryochloris marina MBIC11017

Cited by 7 publications

References 48 publications

Niche partitioning in a cyanobacterium through divergence of its novel chlorophylld-based light-harvesting system

Niche partitioning in a cyanobacterium through divergence of its novel chlorophylld-based light-harvesting system

Does the microbial community play a role in the different colour morphs of the breadcrumb sponge Halichondria panicea?

Experimental and Theoretical Mutation of Exciton States on the Smallest Type-I Photosynthetic Reaction Center Complex of a Green Sulfur Bacterium Chlorobaclum tepidum

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