Isolation and characterization of toxins from brown recluse spider venom (Loxosceles reclusa)

Geren, Collis R.; Chan, Tak K.; Howell, D. E.; Odell, George V.

doi:10.1016/0003-9861(76)90327-1

Cited by 57 publications

(10 citation statements)

References 18 publications

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“…This enzyme also causes cell lysis by hydrolysis of the membrane phospholipid sphingomyelin (Geren et al, 1976;Forrester et al, 1978;Merchant et al, 1998;van Meeteren et al, 2004) and complement activation (Tambourgi et al, 1995(Tambourgi et al, , 1998. We confirmed the presence of sphingomyelinase activity in the five Loxosceles venoms studied.…”

Section: Discussionsupporting

confidence: 89%

Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas

Barbaro¹,

Knysak²,

Martins³

et al. 2005

Toxicon

106

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Section: Discussionsupporting

confidence: 89%

Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas

Barbaro¹,

Knysak²,

Martins³

et al. 2005

Toxicon

106

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“…This difference may be explained by the fact that whereas L. gaucho and L. intermedia are native to Brazil, L. laeta is native to Chile (Gertsch and Ennick, 1983). Geren et al (1973Geren et al ( , 1975Geren et al ( , 1976 working with L. reclusa reported that the venom components responsible for lethal and dermonecrotic activities were two toxins of about 34 kDa. Components with lower molecular weight showed no toxic activity.…”

Section: Discussionmentioning

confidence: 99%

Compared chemical properties of dermonecrotic and lethal toxins from spiders of the genusLoxosceles (Araneae)

et al. 1996

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Loxosceles spider venom usually causes a typical dermonecrotic lesion in bitten patients, but it may also cause systemic effects that may be lethal. Gel filtration on Sephadex G-100 of Loxosceles gaucho, L. laeta, or L. intermedia spider venoms resulted in three fractions (A, containing higher molecular mass components. B containing intermediate molecular mass components, and C with lower molecular mass components). The dermonecrotic and lethal activities were detected exclusively in fraction A of all three species. Analysis by SDS-PAGE showed that the major protein contained in fraction A has molecular weight approximately 35 kDa in L. gaucho and L. intermedia, but 32 kDa in L. laeta venom. These toxins were isolated from venoms of L. gaucho, L. laeta, and L. intermedia by SDS-PAGE followed by blotting to PVDF membrane and sequencing. A database search showed a high level of identity between each toxin and a fragment of the L. reclusa (North American spider) toxin. A multiple sequence alignment of the Loxosceles toxins showed many common identical residues in their N-terminal sequences. Identities ranged from 50.0% (L. gaucho and L. reclusa) to 61.1% (L. intermedia and L. reclusa). The purified toxins were also submitted to capillary electrophoresis peptide mapping after in situ partial hydrolysis of the blotted samples. The results obtained suggest that L. intermedia protein is more similar to L. laeta toxin than L. gaucho toxin and revealed a smaller homology between L. intermedia and L. gaucho. Altogether these findings suggest that the toxins responsible for most important activities of venoms of Loxosceles species have a molecular mass of 32-35 kDa and are probably homologous proteins.

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“…Brown recluse venom is cytotoxic and haemolytic. It contains at least nine components, including enzymes such as hyaluronidase, alkaline phosphotase, esterase, lipase and sphingomyelinidase D2, [1][2][3][4][5] which are responsible for both tissue destruction and haemolysis.…”

Section: Introductionmentioning

confidence: 99%

Epidemiological and clinical manifestations of patients hospitalized with brown recluse spider bite

Dyachenko¹,

Ziv²,

Rozenman³

2006

Acad Dermatol Venereol

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Isolation and characterization of toxins from brown recluse spider venom (Loxosceles reclusa)

Cited by 57 publications

References 18 publications

Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas

Enzymatic characterization, antigenic cross-reactivity and neutralization of dermonecrotic activity of five Loxosceles spider venoms of medical importance in the Americas

Compared chemical properties of dermonecrotic and lethal toxins from spiders of the genusLoxosceles (Araneae)

Epidemiological and clinical manifestations of patients hospitalized with brown recluse spider bite

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