Isolation and Characterization of Tomato Leaf Phyllosphere Methylobacterium and Their Effect on Plant Growth

Senthilkumar, M.; Krishnamoorthy, Rajagopal

doi:10.20546/ijcmas.2017.611.250

Cited by 27 publications

(15 citation statements)

References 53 publications

(45 reference statements)

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“…However, different cultivars of the same plants, carry similar methylotrophic bacterial communities when they grow at the same geographical location (Knief et al 2010b;Krishnamoorthy et al 2018). Several reports have demonstrated the importance and interactions between Methylobacteriumplant species (Sy et al 2001;Lee et al 2006;Madhaiyan et al 2009;Dourado et al 2012;Senthilkumar and Krishnamoorthy 2017).…”

Section: Discussionmentioning

confidence: 99%

Diversity and functional characterization of endophyticMethylobacteriumisolated from banana cultivars of South India and its impact on early growth of tissue culture banana plantlets

et al. 2021

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Aims: This study aimed at determining the distribution, colonization and growth promoting nature of Methylobacterium spp. in tissue culture banana plantlets. Methods and Results: Leaf samples from different field grown banana cultivars were used for Methylobacterium spp., isolation. Metabolic profile and functional characterization for plant growth-promoting traits of the isolates were assessed. The isolates were confirmed using 16S rRNA gene sequencing analysis, which resulted in six distinct species of Methylobacterium namely M. radiotolerans, M. salsuginis, M. thiocyanatum, M. rhodesianum, M. rhodinum and M. populi. Methylobacterium spp. inoculation experiment was conducted under hydroponic system in tissue culture banana plantlets (germ free) with eight selected isolates. A significant increase in growth parameters of Methylobacterium treated plantlets compared to uninoculated control was observed. Methylobacterium salsuginis TNMB03-gfp29 was developed and colonization micrograph was obtained using confocal laser scanning microscopy (CLSM) and scanning electron microscopy in different parts of banana plantlets (root, stem and leaves). Conclusion: Field grown banana plants found to harbour diverse endophytic Methylobacterium population. Our finding suggests that endophytic Methylobacterium species may provide significant plant growth promoting compounds/nutrients to the banana plants. The experimental results demonstrated the efficacy of Methylobacterium spp. as a potential bioinoculant and can be exploited as a phyllosphere and rhizosphere based bioinoculant for the initial establishment and growth of tissue culture banana plantlets. Significance and Impact of the Study: This study extended our knowledge on the distribution of Methylobacterium spp. in banana plants and endophytic colonization nature of this particular genus in plants. In addition, efficient isolate (M. salsuginis TNMB03) identified in this study may be promoted as bio-inoculants for banana plants after field evaluation.

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Section: Discussionmentioning

confidence: 99%

Diversity and functional characterization of endophyticMethylobacteriumisolated from banana cultivars of South India and its impact on early growth of tissue culture banana plantlets

et al. 2021

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“…Hence, plant beneficial and pathogenic species have been identified. It has been shown that facultative methylotrophic bacteria of the genus Methylobacterium are able to promote tomato growth, biomass and fruit yield (Innerebner, Knief, and Vorholt 2011;Senthilkumar and Krishnamoorthy 2017). Bacterial genera like Pseudomonas, Sphingomonas, Bacillus and Methylobacterium have been described as microbial core members on the phyllosphere (Guittar and Shade 2019;Rastogi et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

Runge

Ventura

Kemen

et al. 2021

Preprint

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops, however little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over two consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves. Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

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“…Similar results are in support of the findings of reported that the inoculation of Bacillus amyloliqufaciens and Pseudomonas putida enhanced the vigor index in chickpea under stressed condition and increasing potential to mitigate the stress. Senthil kumar and Krishnamoorthy et al, (2017) reported the influence of Methylobacterium inoculation on increasing the seed germination and vigor index of tomato over un-inoculated control. In addition to this Poorniammal et al, (2020) conducted a field experiment under dry land condition to study the effect of Methylobacterium on seed imbibition to improving vigor index of barnyard millet.…”

Section: Vigor Indexmentioning

confidence: 99%

Comparative Analysis of two Methylobacterium spp. for Mitigating Moisture Stress Induced by PEG in Early Growth Stages of Tomato

Kumutha¹,

Kamaraj²,

Senthilkumar³

2021

Int.J.Curr.Microbiol.App.Sci

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Tomato (Solanum lycopersicum) is widely cultivated vegetable crop and processed throughout the world which is highly susceptible to moisture stress. In this study Methylobacterium spp. viz., M. aminovorans and M. thiocyanatum were used to increase the moisture stress tolerance. Artificial moisture stress was induced to tomato seeds using Polyethylene Glycol (PEG) with concentration ranged from 0 to -3bars.The results showed that highest growth parameters were exhibited in tomato seedlings treated with M.thiocyanatum, where it exhibited maximum germination percentage at 0 bar (42.7%), vigor index at 0 bar (170.8), shoot length at -3bar (2.7 cm),root and seedling length at -2 bar (4.1cm, 5.1cm) followed by M.aminovorans, which showed germination percentage of 40% at (-1.5 bar), vigor index of 131.2 at (-1 bar), root, shoot and seedling length of 2.9cm, 1.9cm, 4.8cm at (-3 bar) respectively. Compared to treated seeds, the un-inoculated control showed lowest germination percentage at 0 bar (17.2%), vigor index (48.3), root, shoot and seedling length (1.7cm, 1.1cm, 2.2cm). Root hair deformation was observed in both treated seedlings which are grown under aseptic condition. In which, M. aminovorans performed well when compared to M. thiocyanatum. The activity of antioxidant enzymes catalase (CAT), peroxidase (PER), proline, glycine betaine (GB) and superoxide dismutase (SOD) were enhanced by M. aminovorans at -1.5 bars & M. thiocyanatum at -2 bars which have the ability to protect the plant under moisture stress conditions. Thus, Methylobacterium has the ability to mitigate drought stress and increase the drought tolerance of tomato crop. K e y w o r d sEnvironmental stresses, CO2 reduction, day neutral plant, tomato

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Isolation and Characterization of Tomato Leaf Phyllosphere Methylobacterium and Their Effect on Plant Growth

Cited by 27 publications

References 53 publications

Diversity and functional characterization of endophyticMethylobacteriumisolated from banana cultivars of South India and its impact on early growth of tissue culture banana plantlets

Diversity and functional characterization of endophyticMethylobacteriumisolated from banana cultivars of South India and its impact on early growth of tissue culture banana plantlets

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

Comparative Analysis of two Methylobacterium spp. for Mitigating Moisture Stress Induced by PEG in Early Growth Stages of Tomato

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