Isolation and characterization of the porcine nuclear factor I (NFI) gene

Meisterernst, Michael; Rogge, Lars; Donath, Cornelia; Gander, Irene; Lottspeich, Friedrich; Mertz, Ronald; Dobner, Thomas; Föckler, Renate; Stelzer, Gertraud; Winnacker, Ernst‐Ludwig

doi:10.1016/0014-5793(88)80279-5

Cited by 29 publications

(11 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different isoforms are generated by multiple alternative splicing of individual NFI genes (Santoro et al, 1988;Inoue et al, 1990;Apt et al, 1994). The Nfi genes encode transcription factors in birds and mammals (Gil et al, 1988;Meisterernst et al, 1988;Paonessa et al, 1988;Santoro et al, 1988;Inoue et al, 1990;Rupp et al, 1990). They are specific DNA-binding proteins that were identified through their roles in adenoviral DNA replication, both in vitro (Nagata et al, 1982;Hurwitz et al, 1985;Leegwater et al, 1985;Rosenfeld et al, 1987) and in vivo (Hay, 1985;Wang and Pearson, 1985).…”

mentioning

confidence: 95%

Nuclear factor I gene expression in the developing forebrain

Plachez

Lindwall

Sunn

et al. 2008

J of Comparative Neurology

View full text Add to dashboard Cite

Three members of the Nuclear Factor I (Nfi) gene family of transcription factors; Nfia, Nfib, and Nfix are highly expressed in the developing mouse brain. Nfia and Nfib knockout mice display profound defects in the development of midline glial populations and the development of forebrain commissures (das Neves et al. [1999] Proc Natl Acad Sci U S A 96:11946-11951; Shu et al. [2003] J Neurosci 23:203-212; Steele-Perkins et al. [2005] Mol Cell Biol 25:685-698). These findings suggest that Nfi genes may regulate the substrate over which the commissural axons grow to reach targets in the contralateral hemisphere. However, these genes are also expressed in the cerebral cortex and, thus, it is important to assess whether this expression correlates with a cell-autonomous role in cortical development. Here we detail the protein expression of NFIA and NFIB during embryonic and postnatal mouse forebrain development. We find that both NFIA and NFIB are expressed in the deep cortical layers and subplate prenatally and display dynamic expression patterns postnatally. Both genes are also highly expressed in the developing hippocampus and in the diencephalon. We also find that principally neither NFIA nor NFIB are expressed in callosally projecting neurons postnatally, emphasizing the role for midline glial cell populations in commissure formation. However, a large proportion of laterally projecting neurons express both NFIA and NFIB, indicating a possible cell-autonomous role for these transcription factors in corticospinal neuron development. Collectively, these data suggest that, in addition to regulating the formation of axon guidance substrates, these genes also have cell-autonomous roles in cortical development.

show abstract

mentioning

confidence: 95%

Nuclear factor I gene expression in the developing forebrain

Plachez

Lindwall

Sunn

et al. 2008

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…NFI was discovered as a protein required for adenovirus DNA replication in vitro (19,20), but it is now clear that NFI proteins play an important role in the expression of many cellular genes (reviewed in reference 8). NFI-C/CTF was the initial NFI gene cloned (24) and contains a prototypical proline-rich transcriptional activation domain, as well as a heptamer repeat homologous to the C-terminal domain of RNA polymerase II (18). Because products of the four NFI genes are often coexpressed (3) and bind to similar DNA sequences (15), it has been difficult to determine the roles of individual NFI family members in gene expression during development.…”

mentioning

confidence: 99%

Essential Role for NFI-C/CTF Transcription-Replication Factor in Tooth Root Development

Steele-Perkins

Butz

Lyons

et al. 2003

Molecular and Cellular Biology

180

228

View full text Add to dashboard Cite

The mammalian tooth forms by a series of reciprocal epithelial-mesenchymal interactions. Although several signaling pathways and transcription factors have been implicated in regulating molar crown development, relatively little is known about the regulation of root development. Four genes encoding nuclear factor I (NFI) transcription-replication proteins are present in the mouse genome: Nfia, Nfib, Nfic, and Nfix. In order to elucidate its physiological role(s), we disrupted the Nfic gene in mice. Heterozygous animals appear normal, whereas Nfic ؊/؊ mice have unique tooth pathologies: molars lacking roots, thin and brittle mandibular incisors, and weakened abnormal maxillary incisors. Feeding in Nfic ؊/؊ mice is impaired, resulting in severe runting and premature death of mice reared on standard laboratory chow. However, a soft-dough diet mitigates the feeding impairment and maintains viability. Although Nfic is expressed in many organ systems, including the developing tooth, the tooth root development defects were the prominent phenotype. Indeed, molar crown development is normal, and well-nourished Nfic ؊/؊ animals are fertile and can live as long as their wild-type littermates. The Nfic mutation is the first mutation described that affects primarily tooth root formation and should greatly aid our understanding of postnatal tooth development.Tooth formation is a complex developmental process that is mediated through a series of epithelial-mesenchymal interactions (29). Several signaling pathways required for early molar tooth development have been identified, including the BMP (31), FGF (13, 27), SHH (5, 10), and WNT (25, 33) pathways. In addition, targeted disruption of a number of transcription factors, including Msx1 and -2 (1), Dlx1 and -2 (22), Pax-9 (21) and others, severely disrupts early tooth development. In contrast, only one pathway regulating late tooth developement has been identified. The Tabby (Ta), Downless (Dl), and Crinkled (Cr) mouse mutations each cause similar defects of postnatal development (32) by disrupting different components of the Ectodysplasin-A hormone signaling system.The nuclear factor I (NFI) family of transcription-replication factors is encoded by four genes in mammals (NFI-A, -B, -C, and -X) and a single gene in Drosophila melanogaster and Caenorhabditis elegans (8,14,23). Prokaryotic homologues of the NFI genes have not been identified. NFI was discovered as a protein required for adenovirus DNA replication in vitro (19,20), but it is now clear that NFI proteins play an important role in the expression of many cellular genes (reviewed in reference 8). NFI-C/CTF was the initial NFI gene cloned (24) and contains a prototypical proline-rich transcriptional activation domain, as well as a heptamer repeat homologous to the C-terminal domain of RNA polymerase II (18). Because products of the four NFI genes are often coexpressed (3) and bind to similar DNA sequences (15), it has been difficult to determine the roles of individual NFI family members in gene expression during deve...

show abstract

“…Since then, the corresponding gene and several highly homologous genes have been cloned in birds and mammals (Santoro et al, 1988;Paonessa, 1988;Gil et al, 1988;Meisterernst et al, 1988;Inoue et al, 1990;Rupp et al, 1990). Sequence analysis of the two Xenopus NFI genes reported here demonstrates that they are the homologs of chicken NFI-B and NFI-C genes, respectively.…”

Section: Fig 9 Anti-nfi-b1 Antibody Supershifts Nfi-dna Complexesmentioning

confidence: 99%

“…The proteins of nuclear factor I (NFI) 1 family are transcription factors encoded by multiple genes in birds and mammals (Gil et al, 1988;Santoro et al, 1988;Meisterernst et al, 1988;Paonessa et al, 1988;Inoue et al, 1990;Rupp et al, 1990). In addition, different forms of these factors can be generated by multiple alternative splicing of individual NFI genes (Santoro et al, 1988;Inoue et al, 1990;Apt et al, 1994), although the functional difference among these various forms is still unclear.…”

mentioning

confidence: 99%

Nuclear Factor I as a Potential Regulator during Postembryonic Organ Development

Puzianowska-Kuźnicka

Shi

1996

Journal of Biological Chemistry

View full text Add to dashboard Cite

Nuclear factor I (NFI) family members are transcription factors that are believed to also participate in DNA replication. We have cloned two Xenopus laevis NFIs that are up-regulated by thyroid hormone. They are 84 -95% identical to their counterparts in birds and mammals. In contrast, the two Xenopus NFIs are much less homologous to each other, sharing only 58% homology, which largely resides in the DNA binding domain at the amino terminus. However, both NFIs can bind to a consensus NFI binding site and activate the transcription of a promoter bearing the site. Northern blot reveals that both NFI genes are regulated in tissue-and developmental stage-dependent manners. They are first activated, independently of thyroid hormone, to low levels at stages 23/24, around the onset of larval organogenesis. After stage 54, their mRNA levels are dramatically upregulated by endogenous thyroid hormone, and high levels of their expression correlate with organ-specific metamorphosis. Furthermore, gel mobility shift assay indicates that the NFI proteins are present in different organs and that their levels are regulated similarly to the mRNA levels. These results strongly suggest that NFIs play important roles during postembryonic organ development, in contrast to the general belief that NFIs are ubiquitous factors.

show abstract

Isolation and characterization of the porcine nuclear factor I (NFI) gene

Cited by 29 publications

References 18 publications

Nuclear factor I gene expression in the developing forebrain

Nuclear factor I gene expression in the developing forebrain

Essential Role for NFI-C/CTF Transcription-Replication Factor in Tooth Root Development

Nuclear Factor I as a Potential Regulator during Postembryonic Organ Development

Contact Info

Product

Resources

About