1988
DOI: 10.1128/iai.56.11.2979-2983.1988
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Isolation and characterization of the localized adherence factor of enteropathogenic Escherichia coli

Abstract: The binding factor of enteropathogenic Escherichia coli O111:H- responsible for localized adherence (LA) on HeLa cells was investigated. Inhibition of LA by carbohydrates and lectins showed that the reactive epitope on HeLa cells contains N-acetylgalactosamine units. Treatment of bacteria with EDTA for extraction of lipopolysaccharides eliminated these polymers as binding factors. Such treatment also caused a marked increase in adhesion suggesting steric hindrance by lipopolysaccharides of the LA factor bindin… Show more

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Cited by 48 publications
(27 citation statements)
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References 31 publications
(22 reference statements)
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“…This would be particularly likely to be the case for the transmembrane porin proteins. The above reactions closely resembled those of Scaletsky et al [10] where an antiserum, absorbed with a non-adhesive strain of E. coli; also reacted predominantly with a 32 kDa protein and to a lesser extent with a 29 kDa OMP. Unfortunately, Scaletsky et al [10] did not provide evidence as to the reaction of their absorbed antiserum with the non-adherent strain used for absorbing the antiserum.…”
Section: Resultssupporting
confidence: 82%
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“…This would be particularly likely to be the case for the transmembrane porin proteins. The above reactions closely resembled those of Scaletsky et al [10] where an antiserum, absorbed with a non-adhesive strain of E. coli; also reacted predominantly with a 32 kDa protein and to a lesser extent with a 29 kDa OMP. Unfortunately, Scaletsky et al [10] did not provide evidence as to the reaction of their absorbed antiserum with the non-adherent strain used for absorbing the antiserum.…”
Section: Resultssupporting
confidence: 82%
“…Strains of E. coli, previously used for studying localised adhesion of EPEC to HEp-2 cells [8], were examined for the presence of the 32 kDa OMP described by Scaletsky et al [10] as the 'localised adherence factor'. The SDS-PAGE OMP profile of strain E20517 (Fig.…”
Section: Resultsmentioning
confidence: 99%
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“…The addition of this sugar during phagocytic assays at 37 or 41³C caused inhibition of phagocytosis of EPEC (Table 3). Since the outer membrane of enter-opathogenic E. coli bears a polypeptide which binds to N-acetylgalactosamine residues on HeLa cells [16] we suggest that this polypeptide can participate in phagocytosis of EPEC. Microorganisms produce multiple adhesins in order to colonize diverse surfaces and these proteins are also involved in non-opsonic phagocytosis of many microbial pathogens.…”
Section: Inhibition Of Lectinophagocytosis Of Epec At Di¡erent Phs Anmentioning
confidence: 85%