Isolation and Characterization of the Major Capsid Protein of Bovine Papilloma Virus Type 1

Abstract

SUMMARYThe major capsid protein of bovine papilloma virus type 1 (BPV-1) was isolated by gel filtration following disruption of purified virus particles with guanidine hydrochloride. The capsid protein, VP1, has a mol. wt. of about 53 500. Amino acid composition studies of VP1 showed that it is a highly acidic protein containing almost twice the average number of acidic residues than basic residues. Relatedness was observed between VP1 and the major capsid proteins of simian virus 40 (SV40) and polyoma virus.

Monoclonal Antibodies to the Major Capsid Protein of Human Papillomavirus Type 1

“…3g, i,j), which were barely or not detected on the stained gels or blots of the disrupted viral particles. These minor species could constitute incomplete disruption products of the virions or degradation products of the major viral polypeptide, as previously discussed (Favre et al, 1975a;Gissmann et al, 1977;Lancaster & Olson, 1982;Meinke & Meinke, 1981 ;Pfister et al, 1977). The anti-rotavirus antibodies showed no reaction with the HPV-1 polypeptides (data not shown) and the polyclonal or monoclonal antibodies detected neither a 76000 nor a 54000 polypeptide in the normal human skin extracts (Fig.…”

“…Viral particles contain a major polypeptide, with a molecular weight in the range of 50000 to 60000, several minor components whose number and origin are unclear and four histone-like polypeptides which are associated with the viral DNA in a minichromosome. (Favre et al, 1975aGissmann et al, 1977;Lancaster & Olson, 1982;Meinke & Meinke, 1981 ;Orth et al, 1977Orth et al, , 1978Pass & Maizel, 1973 ;Pfister et al, 1977). In man, papillomaviruses (PVs) induce benign epithelial tumours of the skin and mucous membranes (warts, condylomas), which may evolve into carcinomas in some instances (Lutzner, 1983;Orth et aL, 1980;zur Hausen, 1981).…”

Expression of the full-length products of the human papillomavirus type 6b (HPV-6b) and HPV-11 L2 open reading frames by recombinant baculovirus, and antigenic comparisons with HPV-11 whole virus particles

“…A number of previous studies have provided evidence that the major constituent of PV capsids is a protein with an average Mr of 54K to 57K encoded by the L1 open reading frame (ORF) (Roseto et al, 1984;Favre et al, 1975;Meinke & Meinke, 1981 ;Pilacinski et al, 1984). In these experiments, L10RF coding capacities have been closely correlated with the size of recombinant L1 products and the major capsid protein present in viral particles.…”

Identification of L2 Open Reading Frame Gene Products of Bovine Papillomavirus Type 1 Using Monoclonal Antibodies

“…The BPV-1 minor capsid protein(s) has been putatively identified as having an Mr of approx. 74K by gel electrophoresis (Meinke & Meinke, 1981). However, the gene products of the BPV-1 L20RF have not been previously identified by antibodies specific for the minor capsid protein.…”