Isolation and characterization of the intracellular MHC class II compartment

Tulp, A.; Verwoerd, Desirée; Dobberstein, Bernhard; Ploegh, Hidde L.; Pieters, Jean

doi:10.1038/369120a0

Cited by 404 publications

(265 citation statements)

References 47 publications

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“…3 It was previously shown that B cell receptor (BCR)-mediated uptake rapidly targets Ags toward multilamellar or multivesicular compartments (1,2), rich in neosynthesized class II molecules and specialized for efficient peptide loading, which were named MHC-II loading compartments (MIIC) (3)(4)(5)(6). Neosynthesized class II molecules are targeted from the secretory trans-Golgi network to early endosomes before reaching MIIC where they accumulate (7,8).…”

mentioning

confidence: 99%

B Cell Receptors and Complement Receptors Target the Antigen to Distinct Intracellular Compartments

Perrin-Cocon

Villiers

Salamero

et al. 2004

The Journal of Immunology

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The processing of exogenous Ags is an essential step for the generation of immunogenic peptides that will be presented to T cells. This processing relies on the efficient intracellular targeting of Ags, because it depends on the content of the compartments in which Ags are delivered in APCs. Opsonization of Ags by the complement component C3 strongly enhances their presentation by B cells and increases their immunogenicity in vivo. To investigate the role of C3 in the targeting of Ags, we compared the intracellular traffic of proteins internalized by complement receptor (CR) and B cell receptor (BCR) in B lymphocytes. Whereas both receptors are able to induce efficient Ag presentation, their intracellular pathways are different. CR ligand is delivered to compartments containing MHC class II molecules (MHC-II) but devoid of transferrin receptor and Lamp-2, whereas BCR rapidly targets its ligand toward Lamp-2-positive, late endosomal MHC-II-enriched compartments through intracellular vesicles containing transferrin receptor. CR and BCR are delivered to distinct endocytic pathways, and the kinetic evolution of the protein content of these pathways is very different. Both types of compartments contain MHC-II, but CR-targeted compartments receive less neosynthesized MHC-II than do BCR-targeted compartments. The targeting induced by CR toward compartments that are distinct from BCR-targeted compartments probably participates in C3 modulation of Ag presentation.

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mentioning

confidence: 99%

B Cell Receptors and Complement Receptors Target the Antigen to Distinct Intracellular Compartments

Perrin-Cocon

Villiers

Salamero

et al. 2004

The Journal of Immunology

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“…The nature of this MHC class II-loading compartment (termed 'MIIC' for MHC class II compartment) has been studied extensively using immunofluorescence or electron microscopy and cell fractionation. 13,63,64 These studies have characterized MIICs as late endosomal compartments containing the late endosomal/lysosomal markers LAMP1, CD63 and partially processed cathepsin D. Since no late endosomes/lysosomes devoid of MHC class II and HLA-DM are observed in MHC class II-expressing APCs, it is thought that MHC class IIloading compartments are conventional endosomal compartments that, in addition, contain the components for MHC class II loading, namely MHC class II and the peptide-loading chaperone HLA-DM. 13,65,66 This implies that classical APCs like macrophages, B cells and DCs have equipped late endosomes for MHC class II loading.…”

Section: Transport Of Autophagosomes To Mhc Class II Loading Compartmmentioning

confidence: 99%

Immune surveillance of intracellular pathogens via autophagy

Schmid

Münz

2005

Cell Death Differ

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MHC class II molecules are thought to present peptides derived from extracellular proteins to CD4 þ T cells, which are important mediators of adaptive immunity to infections. In contrast, autophagy delivers constitutively cytosolic material for lysosomal degradation and has so far been recognized as an efficient mechanism of innate immunity against bacteria and viruses. Recent studies, however, link these two pathways and suggest that intracellular cytosolic and nuclear antigens are processed for MHC class II presentation after autophagy.

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“…7,8 Along the transition from multivesicular to multilaminar MIICs, DM releases the remaining Ii fragment CLIP (class II-associated Ii peptides) from class II dimers and catalyzes the uptake of antigenic peptides. [9][10][11][12] Many antigens such as tumor antigens and certain viral…”

Section: Introductionmentioning

confidence: 99%

C-terminal extension of the MHC class II-associated invariant chain by an antigenic sequence triggers activation of naive T cells

1999

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In vitro and in vivo activation of T cells was investigated T hybridoma cells. Nonprofessional APC expressing with invariant chain-antigen fusion protein. The CD4 T cell recombinant Ii HEL and H2-A k are also able to activate epitope amino acid 52-61 of hen egg lysozyme (HEL) was naive T cells from 3A9 TCR transgenic mice, a result not attached to the C-terminal end of invariant chain (Ii).achieved with peptide pulsed APC. To elicit an in vivo Expression of this recombinant Ii HEL directs the T cell immune response dendritic cells (DC) were transfected epitope to the class II processing pathway. Class II molwith rIi HEL cDNA: following immunization of CBA mice ecules of transfected antigen presenting cells (APC) are with transfected DC, a primary T cell response against the charged with this HEL epitope. The endogenously provided HEL epitope was induced. Thus the procedure described epitope competes with processing and presentation of here could be used to introduce antigens into the class II exogenously added antigen. APC expressing recombinant processing pathway and to elicit T cell activation both in Ii HEL stimulate a maximal IL-2 response of HEL-specific vitro and in vivo.

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Isolation and characterization of the intracellular MHC class II compartment

Cited by 404 publications

References 47 publications

B Cell Receptors and Complement Receptors Target the Antigen to Distinct Intracellular Compartments

B Cell Receptors and Complement Receptors Target the Antigen to Distinct Intracellular Compartments

Immune surveillance of intracellular pathogens via autophagy

C-terminal extension of the MHC class II-associated invariant chain by an antigenic sequence triggers activation of naive T cells

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