Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1

Abstract

ABSTRACT. In this study, the nitrate transporter gene CmNRT1 was isolated from the chrysanthemum variety 'Nannongxuefeng'. The full-length cDNA contains an open reading frame of 1761 bp encoding 587 residues. Using qRT-PCR, we found that CmNRT1 was induced by 10 mM NO 3 -in roots and shoots. Two Arabidopsis thaliana transgenic plants expressing CmNRT1 were selected for functional analyses. Root 15 N influx in wild-type and transgenic A. thaliana lines under 10 or 0.2 mM 15 NO 3 was tested. Our results indicate that CmNRT1 encodes a constitutive component for a lowaffinity transporter.

Genome-Wide Identification of NRT Gene Family and Expression Analysis of Nitrate Transporters in Response to Salt Stress in Poncirus trifoliata

“…AtNRT1.11 and AtNRT1.12 were reported to regulate the redistribution of nitrate content in xylem and phloem [ 18 ]. Moreover, much evidence indicates that NPFs possessed the potential activity of low-affinity nitrate transportation in many species, such as Brassica campestris , chrysanthemum, and rice [ 19 , 20 , 21 ]. As for NRT2s , AtNRT2.1 and AtNRT2.2 were mainly expressed in the epidermis of roots for nitrate absorption [ 22 , 23 ].…”