Isolation and Characterization of the Unit of Electron Transfer in Heart Mitochondria

Green, D. E.; Blair, P.V.; Oda, Takuzo

doi:10.1126/science.140.3565.382

Cited by 19 publications

(10 citation statements)

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“…It was suggested that these isolated elementary particles (EP) correspond to the structures seen by negative staining procedures. FernSndezMorfin (1963) and Green et al (1963) state that the moleculzr weight of this isolated EP is ca. 1.4 X 106 and that (Fern~ndez-Morgm, 1962 b) the "ETP is considered to be a composite of the smaller particles invested with a coat of structural protein."…”

Section: Discussionmentioning

confidence: 93%

“…No attempt has yet been made to carry out correlated biochemical and electron microscopic studies on the insect sarcosome preparations, and thus no experimental basis exists at present for the characterization of the particles from these sarcosomes. Furthermore, although suggestive evidence is accumulating (Fern~ndez-Mor~n, 1963;Green et al 1963) it does not seem that the nature of particles from mammalian tissue has been rigorously demonstrated. However, as a basis for later studies on the structural organization of the insect sarcosome, a brief comparison between the biochemical properties of insect and vertebrate mitochondria may be apposite.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, the stalked parby FcrnSndez-Mor~n (1962 a, b;1963) and Green et al (1963). These authors describe the isolation from beef heart mitochondria of a homogeneous fraction consisting of structures smaller than the ETP units, in which the activities of various components of the electron transfer system are concentrated about three-fold relative to the intact mitochondrion.…”

Section: Discussionmentioning

confidence: 99%

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THE STRUCTURE OF FLIGHT MUSCLE SARCOSOMES IN THE BLOWFLY CALLIPHORA ERYTHROCEPHALA (DIPTERA)

Smith

1963

The Journal of Cell Biology

151

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The electron microscopic structure of sectioned indirect flight muscle fibers of the blowfly Callipkora is described. Particular attention is paid to the organization of the sarcosomes (mitochondria) of this tissue, and this description is accompanied by an account of the appearance of these bodies in negatively stained preparations. In sectioned material, it has been shown that these sarcosomes are similar to other mitochondria in the disposition of the outer and inner limiting membranes, but that the cristae, confluent with the latter, are unusually regular, and form parallel plates, containing circular fenestrations forming cylindrical channels within the matrix. Negatively stained preparations of disrupted sarcosomes reveal that both the outer limiting membrane and the cristae membranes bear large numbers of small particles, similar in appearance to those described by FernfindezMoran and others in various mitochondria. In Calliphora, these particles consist of a subspherical "head" and a cylindrical "stalk," and appear to be arranged on the mitochondrial membranes either randomly distributed, or collected into circular or elongated groups. Recent suggestions concerning the nature of these submitochondrial particles are discussed, and an attempt is made to correlate the aspects of organization of Calliphora sarcosomes, revealed by conventional sectioning of the "intact" structures, and by negative staining of sarcosomal derivatives.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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THE STRUCTURE OF FLIGHT MUSCLE SARCOSOMES IN THE BLOWFLY CALLIPHORA ERYTHROCEPHALA (DIPTERA)

Smith

1963

The Journal of Cell Biology

151

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“…Descriptions of the structure of the mitochondrial system, from different cell typcs, have demonstrated repeating, structural subunits associated with the periphery of the cristae and the mitochondrial membrane surface facing the matrix (4,(6)(7)(8)(9)(10)12). Such subunits have been visualized with the negative stain technique.…”

Section: Introductionmentioning

confidence: 99%

“…A purified mitochondrial fraction, containing particles of the same size as the structural subunits, has been found to contain the components of the electron transport system (6)(7)(8). According to Green et el.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Sonic Oscillation on the Structure and Function of Beef Heart Mitochondria

Stasny

Crane

1964

The Journal of Cell Biology

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Sonic treatment of mitochondria from beef heart results in a distribution of variously sized particles which can be fractionated by differential centrifugation. Electron microscopic observations of negatively stained fractions shows the presence of subunits, which are "knoblike" in appearance, attached to the mitochondrial membrane and membrane derivatives. Such subunits are not completely removed from the membrane by 5 minutes of sonic treatment. However, a fraction containing singular subunits of dimensions similar to those of units attached to the mitochondrial membrane has been observed. Enzymatic activities and cytochrome contents were determined for mitochondria and the fractionated, mitochondrial membrane derivatives. A concentration of enzyme activity and cytochrome content was found in fractions sedimented at 35,300 g and 79,420 g via differential centrifugation in 2x distilled water. The fraction with the highest enzymatic activities and cytochrome content, although rich in mitochondrial membrane derivatives, is deficient in membrane-bound subunits. The fraction with the lowest enzymatic activities and cytochrome content resembles detached mitochondrial subunits when examined in the electron microscope. The biochemical data and the electron microscopic observations suggest that the mitochondrial membrane and not the subunits are responsible for electron transport activity.

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Submikroskopische Cytologie der eukaryotischen Zelle

Sitte

1966

Fortschritte Der Botanik

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Isolation and Characterization of the Unit of Electron Transfer in Heart Mitochondria

Cited by 19 publications

References 0 publications

THE STRUCTURE OF FLIGHT MUSCLE SARCOSOMES IN THE BLOWFLY CALLIPHORA ERYTHROCEPHALA (DIPTERA)

THE STRUCTURE OF FLIGHT MUSCLE SARCOSOMES IN THE BLOWFLY CALLIPHORA ERYTHROCEPHALA (DIPTERA)

The Effect of Sonic Oscillation on the Structure and Function of Beef Heart Mitochondria

Submikroskopische Cytologie der eukaryotischen Zelle

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