Isolation and characterization of the C-class MADS-box gene from the distylous pseudo-cereal Fagopyrum esculentum

Li, Laiyun; Fang, Zhengwu; Li, Xiaofang; Liu, Zhixiong

doi:10.1007/s12374-016-0488-3

Cited by 19 publications

(20 citation statements)

References 36 publications

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“…Similar results were obtained for the overexpression of AG orthologs using transgenic approaches in other plant species as well, such as tobacco [19][20][21][22], tomato [23] and petunia [24]. In addition, the endogenous AG gene function of specifying stamen and carpel identities in the Arabidopsis ag mutant could be partially or fully compensated by ectopic expression of AG orthologs from other plant species, such as MastAG [16], FaesAG [25] and TcAG [26]. These findings indicate that AG orthologs can also be responsible for the formation of reproductive organs.…”

Section: Introductionsupporting

confidence: 73%

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Zhou

et al. 2019

ARCH BIOL SCI BELGRA

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The floral homeotic C-function MADS gene AGAMOUS (AG) in Arabidopsis plays crucial roles in specifying stamen and carpel identities as well as determining floral meristem. However, there have been only a few studies of floral homeotic C-function genes in cucumber thus far. In the present study, CsMADS24, a putative AG ortholog from cucumber, was isolated and characterized. Sequence analysis and protein sequence alignment revealed that the deduced CsMADS24 protein contained the typical MIKC structure and the N-terminal extension, as well as two highly conserved AG motifs (I and II). Phylogenetic analysis showed that CsMADS24 fell into the clade of core eudicots, while being distant from the AG orthologs of basal eudicots, monocots and gymnosperms. Expression analysis by RT-PCR showed that CsMADS24 was exclusively expressed in female flower buds. In situ hybridization revealed that CsMADS24 expression was only detected in the carpels. Functional analyses indicated that the sepals were partly converted into carpelloid-like structures in 35S::35S::CsMADS24 transgenic plants. In addition, earlier flowering and delayed floral organ abscission during the development of siliques were also observed in transgenic Arabidopsis. Our findings demonstrate that the AG ortholog plays an exclusive role in carpel specification of cucumber, providing a basis for revealing the mechanisms of reproductive development in cucumber.

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Section: Introductionsupporting

confidence: 73%

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Zhou

et al. 2019

ARCH BIOL SCI BELGRA

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“…Our analysis of floral biometry showed two reciprocal herkogam morphs, with a ratio stamenpistil greater than 1.25 and of less than 0.9, matching the most important Barrett's (2019) criteria for functional herkogamy, and the pistils of the morph-1 flowers always 1-2 mm smaller than the pistils of the morph-2, also matching a secondary criteria of heteromorphic system (Barrett 2019). The floral characteristics we found in L.g.hexapetala corresponded to two other well-known distylous species: Fagopyrum esculentum (Li et al, 2017) and Linum suffruticosum (Ruiz-Martín et al, 2018). Both of these species show a non-tubular dystilous flower structure, we also found in L.g.hexapetala (Fig.…”

Section: First Evidence Of a Self-incompatibility System Found In L supporting

confidence: 63%

“…Distylous species genetically express two types of floral morphs, differing in their reciprocal heights of styles and stamen (reciprocal herkogamy). For example, in Primroses (Primula veris & Primula vulgaris), Buckwheat (Fagopyrum esculentum) and Lungwort (Pulmonaria officinalis), individuals develop either long-styled or short-styled morphs, with short and long stamens respectively Jacquemyn, Endels, Brys, Hermy, & Woodell, 2009;Li, Fang, Li, & Liu, 2017;Meeus, Brys, Honnay, & Jacquemyn, 2013). Tristylous species genetically express three types of floral morphs: long-styled, mid-styled and short-styled morphs, again differing in their stigma and anther/stamen heights.…”

Section: Introductionmentioning

confidence: 99%

Self-incompatibility limits sexual reproduction rather than environmental conditions in an invasive aquatic plant

Lemus¹,

Bozec²,

Harang³

et al. 2020

Preprint

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SummaryFruitfulness and fertility are important components of sexual reproductive success in plants, and often depends on environmental conditions and reproductive systems. For invasive plants, fruitfulness and fertility control their ecological success and adaptation in invaded ecosystems. We studied which factors bring about fruitfulness and fertility in invasive populations of the aquatic plant Ludwigia grandiflora subsp. hexapetala.We analysed fruitfulness and fertility of 37 populations growing under variable climatic conditions in Western Europe, and sub-sampled fruitful and fruitless populations grown in common controlled conditions. We carried out self- and cross-pollinations and measured their floral biometrics.Environmental conditions, and temperature in particular, did not affect fruitfulness and fertility in-situ or in common controlled environments. Hand-pollinations resulted in fruit production by individuals sampled from fruitless populations when pollen came from fruitful populations, and by individuals sampled from fruitful populations whatever the origin of pollen. Floral biometrics evidenced the existence of two floral morphs that overlapped with fruitfulness, and individual incompatibility.Our results rebutted the hypothesis that environmental conditions control fruitfulness and fertility in these invasive populations. We instead found that fruit and seed production were controlled by a reproductive system involving a self-incompatible approach herkogamous morph and a self-compatible reverse herkogamous morph. We assessed the floral morphs distribution worldwide of fruitless and fruitful native and invasive populations that matched our results at larger scale. Our results may constitute the first evidence of a possible heteromorphic self-incompatible system in Ludwigia populations and in Onagraceae phylogeny. It calls for further investigations on reproductive systems in this plant family. Finally, the observation that the self-incompatible morph seemed to be the world most invasive morph in this species tackles our understanding of biological and ecological conditions for invasiveness.Synthesis. Our study showed that fruitfulness and fertility in the aquatic invasive plant, Ludwigia grandiflora subsp. hexapetala depend on a self-incompatibility system coinciding with two floral morphs, rather than environmental conditions and limitations. These new explanations on the sexual success of Ludwigia invasive populations will help defining new predictions about its worldwide spreads and ecological success, and will help reappraising future management plans.

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“…Plasmid pBI121(negative control) was also transformed into homozygous ap1-10 Arabidopsis using the same protocol described above. Transgenic Arabidopsis seeds were selected and seedlings were cultivated according to Li et al [25]. Moreover, transgenic A. thaliana lines were confirmed by PCR and qPCR as described above using the same gene-specific primers for MapaAGL6-1 and MapaAGL6-2, respectively.…”

Section: Vector Construction and Arabidopsis Transformationmentioning

confidence: 99%

Characterization of Two AGL6–Like Genes from a Chinese Endemic Woody Tree, Manglietia patungensis (Magnoliaceae) Provides Insight into Perianth Development and Evolution in Basal Angiosperms

Liu

Zhang

et al. 2019

Forests

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Manglietia patungensis (Magnoliaceae) exhibits radially symmetric flowers with perianth consisting of three separate sepaloid tepals in whorl 1 and six petaloid tepals in the inner two whorls, which shows an obvious difference from flowers of most Magnoliaceae species that contain three uniform petaloid tepals whorls, and make it an excellent model for understanding perianth morphology differentiation during early flower evolution. Here, two AGL6 orthologs, MapaAGL6-1 and MapaAGL6-2, were isolated from M. patungensis. Sequence alignment and phylogenetic analyses grouped both genes into the AGL6 lineage. MapaAGL6-1 is expressed only in the perianth whorls, while MapaAGL6-2 is strongly expressed in the perianth whorls but is lowly expressed in gynoecium. Furthermore, ectopic expression of MapaAGL6-1 results in strong complementation phenotypes in the Arabidopsis ap1-10 flower and production of normal floral organs in four floral whorls only with the petal number reduced in whorl 2, while ectopic expression of MapaAGL6-2 only results in petals partly rescued but failing to terminate carpelloid development in Arabidopsis ap1-10 mutant. In addition, the daughter lines generated from a cross between 35S::MapaAGL6-1 transgenic plants showing strong phenotypes and 35S::MapaAGL6-2 transgenic plants showing phenotypic changes produce normal flowers. Our results suggest that MapaAGL6-1 is a reasonable A-function gene controlling perianth identity in Magnoliaceae, which infers from its expression region and complementation phenotypes in Arabidopsis ap1 mutant, while MapaAGL6-2 is mainly involved in petaloid tepal development. Our data also provide a new clue to uncover the perianth development and early evolution in basal angiosperms.

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Isolation and characterization of the C-class MADS-box gene from the distylous pseudo-cereal Fagopyrum esculentum

Cited by 19 publications

References 36 publications

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Ectopic expression of CsMADS24, an AGAMOUS ortholog from cucumber, causes homeotic conversion of sepals into carpels in transgenic arabidopsis plants

Self-incompatibility limits sexual reproduction rather than environmental conditions in an invasive aquatic plant

Characterization of Two AGL6–Like Genes from a Chinese Endemic Woody Tree, Manglietia patungensis (Magnoliaceae) Provides Insight into Perianth Development and Evolution in Basal Angiosperms

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