Isolation and Characterization of the <i>mheA</i> (Most Highly Expressed) Gene of <i>Aspergillus oryzae</i>

Liu, Pengcheng; Lim, Ji Young; Kim, Hee Seo; Kim, Jong Hwa; Chae, Keon Sang

doi:10.5941/myco.2012.40.3.208

Cited by 1 publication

(1 citation statement)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The top eight most highly expressed genes (unique genes, no duplicates) were selected as candidate strong promoters. Additionally, pdcA, which appeared at #15 in the rank of this list was selected as other studies suggest this is one of the most highly expressed genes in A. oryzae 109 . thiA, which was ranked #20 in this list, was also selected, because it has successfully been used for overexpression in A. oryzae previously 49 .…”

Section: Identification Of Core Promoters For Expression In a Oryzae ...mentioning

confidence: 99%

Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit

Maini Rekdal,

van der Luijt,

Chen

et al. 2024

Nat Commun

View full text Add to dashboard Cite

Filamentous fungi are critical in the transition to a more sustainable food system. While genetic modification of these organisms has promise for enhancing the nutritional value, sensory appeal, and scalability of fungal foods, genetic tools and demonstrated use cases for bioengineered food production by edible strains are lacking. Here, we develop a modular synthetic biology toolkit for Aspergillus oryzae, an edible fungus used in fermented foods, protein production, and meat alternatives. Our toolkit includes a CRISPR-Cas9 method for gene integration, neutral loci, and tunable promoters. We use these tools to elevate intracellular levels of the nutraceutical ergothioneine and the flavor-and color molecule heme in the edible biomass. The strain overproducing heme is red in color and is readily formulated into imitation meat patties with minimal processing. These findings highlight the promise of synthetic biology to enhance fungal foods and provide useful genetic tools for applications in food production and beyond.

show abstract

Section: Identification Of Core Promoters For Expression In a Oryzae ...mentioning

confidence: 99%

Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit

Maini Rekdal,

van der Luijt,

Chen

et al. 2024

Nat Commun

View full text Add to dashboard Cite

show abstract

Isolation and Characterization of the mheA (Most Highly Expressed) Gene of Aspergillus oryzae

Cited by 1 publication

References 4 publications

Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit

Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit

Contact Info

Product

Resources

About