Isolation and characterization of the immunostimulating β-glucans of an edible mushroom Termitomyces robustus var.

Bhanja, Sunil K.; Nandan, Chanchal K.; Mandal, Soumitra; Bhunia, Bibhas K.; Maiti, Tapas K.; Mondal, Soumitra; Islam, Syed S.

doi:10.1016/j.carres.2012.04.007

Cited by 53 publications

(30 citation statements)

References 38 publications

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“…In the present investigation another water soluble polysaccharide (ELPS) has been isolated from the aqueous extract of this mushroom and characterized as ␤-glucan. Similar type of ␤-glucan was isolated by Bhanja et al (2012) but differing in the number of sugar moieties and linkages in the skeleton chain. The water insoluble ␤-glucan (PS-II) isolated by Bhanja et al (2012) contained (1 → 3)-linked three glucose moieties in the skeleton chain with branching at C-6 by another glucose residue, where as the present material is a water soluble ␤-glucan (ELPS) consisting of five (1 → 3)-, (1 → 6)-linked glucose moieties in the skeleton chain with branching at C-6 by another one.…”

Section: Introductionmentioning

confidence: 76%

“…Similar type of ␤-glucan was isolated by Bhanja et al (2012) but differing in the number of sugar moieties and linkages in the skeleton chain. The water insoluble ␤-glucan (PS-II) isolated by Bhanja et al (2012) contained (1 → 3)-linked three glucose moieties in the skeleton chain with branching at C-6 by another glucose residue, where as the present material is a water soluble ␤-glucan (ELPS) consisting of five (1 → 3)-, (1 → 6)-linked glucose moieties in the skeleton chain with branching at C-6 by another one. In the present investigation detailed structural characterization, immunological studies and antioxidant properties of the ELPS have been carried out and reported herein.…”

Section: Introductionmentioning

confidence: 76%

“…Macrophage, splenocyte and thymocyte proliferation assays were determined by adopting the procedures as described previously (Bhanja et al, 2012;Nandi et al, 2013). The formulas to calculate the SPI and TPI are as follows:…”

Section: Immunostimulating Propertiesmentioning

confidence: 99%

“…All the chemical shift values of three D residues were same except the chemical shift values of C-6. Among the three D residues, one moiety (D I ) was glycosidically linked to the most rigid part C, hence, its C-6 signal (ı 68.9) showed 0.1 ppm downfield shift with respect to another two residues of D II (ı 68.8) due to neighboring effect (Bhanja et al, 2012) of the rigid part C. The linking at C-6 of the residues C and D were further confirmed by DEPT-135 spectrum (Fig. 2b).…”

Section: Nmr and Structural Analysis Of The Elpsmentioning

confidence: 99%

See 3 more Smart Citations

Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum

Maity

Sen

Maji

et al. 2015

Carbohydrate Polymers

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Section: Introductionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 76%

Section: Immunostimulating Propertiesmentioning

confidence: 99%

Section: Nmr and Structural Analysis Of The Elpsmentioning

confidence: 99%

See 2 more Smart Citations

Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum

Maity

Sen

Maji

et al. 2015

Carbohydrate Polymers

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“…Likewise, glucans are involved in various biological processes such as immunostimulation effects, suppressing cancer cells development [8,9] and drug delivery [4]. It was shown that glucans modulated immunes effects regardless of their structural complexity such as β(1 → 3), β(1 → 4) or β(1 → 6) linkages Interestingly, various studies have pointed out that higher degree of structural complexity is coupled with higher immunomodulatory, antioxidant, and anti-cancer effects [3,6,[10][11][12][13][14][15][16]. However these effects are not the only ones.…”

Section: Introductionmentioning

confidence: 99%

Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation

Larguech¹,

Césaro²,

Toumi³

et al. 2017

Anat Physiol

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Beta-(1 → 3)-glucans constitute highly promising biomolecules as evidenced by their immunostimulating ability which were first used as folk medicine in Chinese populations and further identified in fungi, yeasts and seaweeds. Previous investigations showed that glucans proteins interaction mediate cell growth pathways. We have adopted this approach to study the effect of laminarin, a beta-(1 → 3)-D-glucan, on Mesenchymal Stem Cells (MSCs) proliferation and differentiation. MSCs were cultured in MSC growth and chondrogenic differentiation mediums. Proliferation rate and apoptosis were explored by cell count, MTT assays and Annexin V staining. mRNA and protein expression of specifics markers for MSCs and chondrocytes were studied using qPCR and immunofluorescence. Results showed that laminarin treatment reduced MSCs proliferation in both growth and chondrogenic mediums (p<0.05). Annexin V staining showed no apoptosis. MSC cells in growth medium showed no impact of laminarin for Thy1, nucleostemin and endoglin mRNA analysis. Conversely, in chondrogenetic medium, laminarin had a negative effect on Thy1 levels and no change in nucleostemin and endoglin. Collagen II responded positively in chondrogenitic medium in absence of laminarin and significantly reduced when laminarin was added (p<0.05). These results indicate that laminarin inhibited both cells proliferation and chondrogenic differentiation suggesting potential clinical applications in MSC therapy.

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Chromatography in characterization of polysaccharides from medicinal plants and fungi

Cheong

Zhao

et al. 2012

J of Separation Science

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Polysaccharides isolated from medicinal plants and fungi exhibit multiple pharmacological activities. The biological activities of polysaccharides depend on their chemical characteristics. However, characterization of polysaccahrides is a challenge because of their complicated structure and macromolecular mass. In this review, chromatography in characterization of polysaccharides, including physicochemical characterization (purity, molecular mass, and distribution), structural characterization (constituent monosaccharide composition and the ratio, the features of glycosidic linkages), and fingerprint of polysaccharides (acidic and enzymatic hydrolysates), from medicinal plants and fungi were reviewed and discussed according to the publications collected in Web of Science since 2007. The perspective for characterization of polysaccharides has also been described.

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Isolation and characterization of the immunostimulating β-glucans of an edible mushroom Termitomyces robustus var.

Cited by 53 publications

References 38 publications

Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum

Structural, immunological, and antioxidant studies of β-glucan from edible mushroom Entoloma lividoalbum

Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation

Chromatography in characterization of polysaccharides from medicinal plants and fungi

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