Isolation and characterization of the TaSnRK2.10 gene and its association with agronomic traits in wheat (Triticum aestivum L.)

Abstract

Sucrose non-fermenting 1-related protein kinases (SnRKs) comprise a major family of signaling genes in plants and are associated with metabolic regulation, nutrient utilization and stress responses. This gene family has been proposed to be involved in sucrose signaling. In the present study, we cloned three copies of the TaSnRK2.10 gene from bread wheat on chromosomes 4A, 4B and 4D. The coding sequence (CDS) is 1086 bp in length and encodes a protein of 361 amino acids that exhibits functional domains shared with SnRK2s. Based on the haplotypes of TaSnRK2.10-4A (Hap-4A-H and Hap-4A-L), a cleaved amplified polymorphic sequence (CAPS) marker designated TaSnRK2.10-4A-CAPS was developed and mapped between the markers D-1092101 and D-100014232 using a set of recombinant inbred lines (RILs). The TaSnRK2.10-4B alleles (Hap-4B-G and Hap-4B-A) were transformed into allele-specific PCR (AS-PCR) markers TaSnRK2.10-4B-AS1 and TaSnRK2.10-4B-AS2, which were located between the markers D-1281577 and S-1862758. No diversity was found for TaSnRK2.10-4D. An association analysis using a natural population consisting of 128 winter wheat varieties in multiple environments showed that the thousand grain weight (TGW) and spike length (SL) of Hap-4A-H were significantly higher than those of Hap-4A-L, but pant height (PH) was significantly lower.

Genome-wide association mapping of bread wheat genotypes for sustainable food security and yield potential under limited water conditions

“…The TaSnRK2 gene determined sucrose non-fermenting 1-related protein kinase situated at chromosomes 4A and 4B. Its role is important for responding against several environmental conditions and depict significant association with SPS and GPS [ 18 , 59 , 60 ]. Wheat yield related genes, such as TaGW2-A1 at 6A TaTGW6-A1 at 3A, TaCwi at 2A, TaGS5-A1/TaGS-D1 at 4A, TaSus1 at 7A and TaSus2 at 7B reported by wheat scientists [ 40 , 61 , 62 ].…”

Identification and Validation of New Stable QTLs for Grain Weight and Size by Multiple Mapping Models in Common Wheat

“…Therefore, the PM H + -ATPase is a component of the auxin-signaling cascade that may direct pattern formation in embryos. Moreover, several reported genes related to TGW belong to the serine/threonine-protein kinase family, such as TaSnRK2.3 ( Miao et al, 2017 ), TaSnRK2.9 ( Rehman et al, 2019 ), TaSnRK2.10 ( Zhang Z. et al, 2017 ), and Tasg-D1 ( Cheng et al, 2020 ), implying that the serine/threonine-protein kinase proteins play important roles in regulation of wheat grain development. Taken together, TraesCS4B02G376400 and TraesCS4B02G376800 are likely to be candidate genes of Qtgw.ahau-4B.2 .…”

“…6-SFT-A2 is involved in fructan biosynthesis and significantly associated with TGW under rainfed conditions ( Yue et al, 2015 ). TaSnRK2.3 , TaSnRK2.9 , and TaSnRK2.10 , three members of the SnRK2 family, are associated with TGW ( Miao et al, 2017 ; Zhang Z. et al, 2017 ; Rehman et al, 2019 ). More recently, Cheng et al (2020) isolated the Tasg-D1 gene, which encodes serine/threonine protein kinase glycogen synthase kinase 3 (STKc_GSK3), leading to formation of round grains in Indian dwarf wheat ( Triticum sphaerococcum Perc.).…”

A Genome-Wide Association Study of Wheat Spike Related Traits in China

“…The TaSnRK2 gene encoded sucrose non-fermenting 1-related protein kinase was detected on chromosome 4A/4B/4D. It plays crucial roles in response to various environment stimuli and shows significant correlation to spike length and thousand kernel weight (Miao et al, 2017 ; Zhang et al, 2017 ). AGO1d gene mutant in a tetraploid durum wheat produced shorter spikes and fewer kernels per spike than wild-type controls (Feng et al, 2017 ).…”