Isolation and characterization of tetranucleotide microsatellites from the leatherback turtle (Dermochelys coriacea)

Alstad, Travis I.; Shamblin, Brian M.; Bagley, Dean A.; Ehrhart, Llewellyn M.; Nairn, Campbell J.

doi:10.1007/s12686-010-9378-z

Cited by 5 publications

(4 citation statements)

References 17 publications

(20 reference statements)

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“…Besides the four new markers developed in this study (see above), we also analyzed P186, Dc99 [47], Nigra32 [48], LB141 [37], Derm 5 and Derm 34 [38]. Part of the genotyping (Dc003, Dc005, Dc013, P186, LB141, Derm5 and Derm34) was performed on a Beckman Coulter automated sequencer, using pre-labeled primers with labels D2, D3 or D4.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we aimed to investigate the recent demographic history and the current fine-scale structure of the NW Atlantic Ocean RMU using the most recent markers, including some recently published [37], [38] and sensitive analytical methods [33], [39], [40]. We focused on three nesting rookeries that are very different in terms of population sizes and recent trends in nesting activities.…”

Section: Introductionmentioning

confidence: 99%

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Recent Demographic History and Present Fine-Scale Structure in the Northwest Atlantic Leatherback (Dermochelys coriacea) Turtle Population

Molfetti

Vilaça²,

Georges³

et al. 2013

PLoS ONE

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The leatherback turtle Dermochelys coriacea is the most widely distributed sea turtle species in the world. It exhibits complex life traits: female homing and migration, migrations of juveniles and males that remain poorly known, and a strong climatic influence on resources, breeding success and sex-ratio. It is consequently challenging to understand population dynamics. Leatherbacks are critically endangered, yet the group from the Northwest Atlantic is currently considered to be under lower risk than other populations while hosting some of the largest rookeries. Here, we investigated the genetic diversity and the demographic history of contrasted rookeries from this group, namely two large nesting populations in French Guiana, and a smaller one in the French West Indies. We used 10 microsatellite loci, of which four are newly isolated, and mitochondrial DNA sequences of the control region and cytochrome b. Both mitochondrial and nuclear markers revealed that the Northwest Atlantic stock of leatherbacks derives from a single ancestral origin, but show current genetic structuration at the scale of nesting sites, with the maintenance of migrants amongst rookeries. Low nuclear genetic diversities are related to founder effects that followed consequent bottlenecks during the late Pleistocene/Holocene. Most probably in response to climatic oscillations, with a possible influence of early human hunting, female effective population sizes collapsed from 2 million to 200. Evidence of founder effects and high numbers of migrants make it possible to reconsider the population dynamics of the species, formerly considered as a metapopulation model: we propose a more relaxed island model, which we expect to be a key element in the currently observed recovering of populations. Although these Northwest Atlantic rookeries should be considered as a single evolutionary unit, we stress that local conservation efforts remain necessary since each nesting site hosts part of the genetic diversity and species history.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Recent Demographic History and Present Fine-Scale Structure in the Northwest Atlantic Leatherback (Dermochelys coriacea) Turtle Population

Molfetti

Vilaça²,

Georges³

et al. 2013

PLoS ONE

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“…Informative nuclear markers have also now been developed for all marine turtle species, offering finer-scale resolution in some biological contexts, and applicability to research questions about the male component of populations. This has included informative microsatellites (see Glossary; e.g., Aggarwal et al, 2004;Jensen et al, 2006;Shamblin et al, 2007Shamblin et al, , 2009Shamblin et al, , 2012cAlstad et al, 2011;Roden and Dutton, 2011) and more recently, single nucleotide polymorphisms (SNPs; Roden et al, 2009;Hancock-Hanser et al, 2013;Hurtado et al, 2016). With many alleles at each locus, nuclear microsatellites can be employed for applications such as individual and familial genotyping (Selkoe and Toonen, 2006).…”

Section: Expansion Of Molecular Markersmentioning

confidence: 99%

Advances in the Application of Genetics in Marine Turtle Biology and Conservation

et al. 2017

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Marine turtles migrate across long distances, exhibit complex life histories, and occupy habitats that are difficult to observe. These factors present substantial challenges to understanding fundamental aspects of their biology or assessing human impacts, many of which are important for the effective conservation of these threatened and endangered species. The early development and application of genetic tools made important contributions to understanding marine turtle population and evolutionary biology, such as providing evidence of regional natal homing by breeding adults, establishing connectivity between rookeries and foraging habitats, and determining phylogeography and broad scale stock structure for most marine turtle species. Recent innovations in molecular technologies, statistical methods, and creative application of genetic tools have significantly built upon this knowledge to address key questions in marine turtle biology and conservation management. Here, we evaluate the latest major advances and potential of marine turtle genetic applications, including improved resolution and large-scale syntheses of population structure, connectivity and phylogeography, estimation of key demographic rates such as age to maturity and operational or breeding sex ratios, insight into reproductive strategies and behavior, and assessment of differential human impacts among populations. We then discuss remaining challenges and emerging capabilities, such as rapid, multiplexed genotyping, and investigation of the genomic underpinnings of adaptive variation afforded by high-throughput sequencing technologies.

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“…No total, 10 loci de microssatélites já desenvolvidos para tartarugas marinhas foram utilizados nas análises. São eles: Nigra200 (Dutton 1995), Cm3, Cm58, Cm72, Cc117 (Fitzsimmons et al 1995, Cc7 (Fitzsimmons 1998), OR2, OR7 (Aggarwal et al 2004), LB141 (Roden & Dutton 2011) e Derm5 (Alstad et al 2011).…”

Section: Extração E Amplificação Dos Microssatélitesunclassified

Estrutura populacional e história demográfica da tartaruga-verde (Chelonia mydas) no Atlântico Oeste

Jordão¹

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Whatever you do, enjoy yourself". Ouvi esta frase no começo de 2013 em Baltimore, no simpósio internacional de tartarugas marinhas. A palestra explorava as conexões entre as pessoas e o amor pelo que se faz, imprescindíveis em um campo de estudo multidisciplinar cada vez mais exigente: a conservação. Agradeço as conexões que me trouxeram até aqui, a experiência, a oportunidade de viver o novo, de novo, quantas vezes forem necessárias. À professora Lurdes Toledo, por ter me aceitado em seu laboratório, por sua confiança em mim e por sempre me incentivar, com tanta gentileza. Muito obrigada! Aos amigos que fiz no laboratório de peixes, onde aprendi tanta coisa: Mari, Rivi, Anita, Ricardo, Rodrigo, Carlos, Fê, Carol; foi um prazer ter convivido com vocês, cada um à sua maneira. Mari, fazer PCRs em sua companhia significava uma tarde de boa conversa. Rivi, impossível ficar séria ao seu lado! Obrigada pelas sugestões que oferecia com tanto bom humor. Fê, sou muito grata à confiança e grande ajuda nos meus primeiros passos na pós-graduação. Seus ensinamentos foram fundamentais neste processo. Rodrigo e suas aventuras no laboratório, sempre motivo de muitas risadas! Carlos, obrigada pela ajuda em todos os momentos, nas coletas, no dia-a-dia, por ter sempre um sorriso no rosto e por torcer sinceramente por cada um de nós. Não é à toa que gostamos tanto de você! Carol, obrigada pela amizade, passeios, as coletas de que pude participar, almoços, cervejinhas, nossas longas conversas no laboratório. É muito bom saber que nossa amizade ultrapassou os limites do laboratório. Aos amigos que fiz no departamento de genética: Ju, Rê, Lê, Lili, Danilo e Adam; obrigada pelo apoio no exame de ingresso, disciplinas, qualificação, projetos e conversas. Esta dissertação tem um pouquinho de vocês em suas páginas. À Ana Cris Bondioli, que também aceitou o desafio de me orientar, sabendo que eu não tinha experiência prévia com genética nem com animais marinhos, mas que acreditou em mim. E acreditou tanto que com sua ajuda ampliei minhas perspectivas profissionais e pessoais; não tenho palavras para agradecer nossas conversas sobre biologia, viagens, culinária, arte, música, planos futuros, o seu cuidado e amizade! Além disso, ganhei mais duas companhias, Jeff Collacico e Juju, e vocês se tornaram minha segunda família em São Paulo. Ao Benoit de Thoisy, pela orientação e por ter me recebido tão bem na Guiana Francesa. Lá eu tive a oportunidade de trabalhar dentro do Instituto Pasteur, viajar por praticamente toda a costa do país, conhecer a floresta amazônica, cruzar rios, presenciar a desova da tartaruga-verde de madrugada, dormir em uma tribo indígena, receber um jantar incrível de despedida, arriscar no francês e vivenciar uma cultura tão diferente e rica. Que sorte a minha! À Katia Slama, por sua amizade, pelos nossos almoços aos domingos, vinhos, trilhas, praias, músicas, conversas; sua companhia fez minha estadia tão agradável! À Eugenia Naro-Maciel, por ter me indicado para a experiência na Guiana Francesa; também à Gisele Lobo Hajdu...

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Isolation and characterization of tetranucleotide microsatellites from the leatherback turtle (Dermochelys coriacea)

Cited by 5 publications

References 17 publications

Recent Demographic History and Present Fine-Scale Structure in the Northwest Atlantic Leatherback (Dermochelys coriacea) Turtle Population

Recent Demographic History and Present Fine-Scale Structure in the Northwest Atlantic Leatherback (Dermochelys coriacea) Turtle Population

Advances in the Application of Genetics in Marine Turtle Biology and Conservation

Estrutura populacional e história demográfica da tartaruga-verde (Chelonia mydas) no Atlântico Oeste

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