Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp. HS21 and Acinetobacter sp. HS51

Zhang, Weiwei; Wen, Ying-Ying; Niu, Zongliang; Yin, Kun; Xu, Dongxue; Chen, Lingxin

doi:10.1007/s11274-011-0834-z

Cited by 53 publications

(21 citation statements)

References 29 publications

(31 reference statements)

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“…Untreated control soils showed no degradation of the applied sulfamethazine. Although microbial degradation of sulfonamides (24,30) and even the utilization of sulfonamides as the only carbon and energy source have been reported (31), detailed knowledge concerning the bacterial strains involved and particularly the extent to which degradation is accomplished is still lacking. Just one study providing profound information about both topics, the microbial sulfonamide degradation and the bacteria involved, was quite recently published by Topp et al (29).…”

Section: Discussionmentioning

confidence: 99%

Degradation of Sulfadiazine by Microbacterium lacus Strain SDZm4, Isolated from Lysimeters Previously Manured with Slurry from Sulfadiazine-Medicated Pigs

Tappe

Herbst

Hofmann

et al. 2013

Appl Environ Microbiol

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b Sulfadiazine (SDZ)-degrading bacterial cultures were enriched from the topsoil layer of lysimeters that were formerly treated with manure from pigs medicated with 14 C-labeled SDZ. The loss of about 35% of the applied radioactivity after an incubation period of 3 years was attributed to CO 2 release due to mineralization processes in the lysimeters. Microcosm experiments with moist soil and soil slurries originating from these lysimeters confirmed the presumed mineralization potential, and an SDZ-degrading bacterium was isolated. It was identified as Microbacterium lacus, denoted strain SDZm4. During degradation studies with M. lacus strain SDZm4 using pyrimidine-ring labeled SDZ, SDZ disappeared completely but no 14 CO 2 was released during 10 days of incubation. The entire applied radioactivity (AR) remained in solution and could be assigned to 2-aminopyrimidine. In contrast, for parallel incubations but with phenyl ring-labeled SDZ, 56% of the AR was released as 14 CO 2 , 16% was linked to biomass, and 21% remained as dissolved, not yet identified 14 C. Thus, it was shown that M. lacus extensively mineralized and partly assimilated the phenyl moiety of the SDZ molecule while forming equimolar amounts of 2-aminopyrimidine. This partial degradation might be an important step in the complete mineralization of SDZ by soil microorganisms.

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Section: Discussionmentioning

confidence: 99%

Degradation of Sulfadiazine by Microbacterium lacus Strain SDZm4, Isolated from Lysimeters Previously Manured with Slurry from Sulfadiazine-Medicated Pigs

Tappe

Herbst

Hofmann

et al. 2013

Appl Environ Microbiol

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“…Supernatants were obtained by filtering the mixture through 0.45-μm nylon membrane prior to HPLC analysis. To determine whether the reduced DES was due to accumulation in bacterial cells, DES associated with the bacterial cells was detected as described by Zhang et al (2012) with minor modification. Cell pellet was resuspended in 200-μl protein denaturing buffer (100 mM NaH 2 PO 4 , 10 mM Tris-Cl, 8 M urea, adjusted pH to 8.0 using NaOH) and lysed for 1 h by gently shaking.…”

Section: Des Degradation By Bacterial Strainmentioning

confidence: 99%

Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

Zhang

Niu

Liao

et al. 2013

Appl Microbiol Biotechnol

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Since diethylstilbestrol (DES) interrupts endocrine systems and generates reproductive abnormalities in both wildlife and human beings, methods to remove DES from the environments are urgently recommended. In this study, bacterial strain J51 was isolated and tested to effectively degrade DES. J51 was identified as Pseudomonas sp. based on its nucleotide sequence of 16S rRNA. The quinoprotein alcohol dehydrogenase and isocitrate lyase were identified to be involved in DES degradation by MALDI-TOF-TOF MS/ MS analysis. In the presence of 40 mg/l DES, increase of the genes encoding quinoprotein alcohol dehydrogenase and isocitrate lyase in both RNA and protein levels was determined. The HPLC/MS analysis showed that DES was hydrolyzed to a major degrading metabolite DES-4-semiquinone. It was the first time to demonstrate the characteristics of DES degradation by specific bacterial strain and the higher degradation efficiency indicated the potential application of Pseudomonas sp. strain J51 in the treatment of DES-contaminated freshwater and seawater environments.

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“…Ultrafiltrated supernatant was subjected to LCQ Fleet high sensitivity multistage ion trap mass spectrometer system. MS was operated in the electron spray ionization mode with a positive polarity and scanned by normal mass range from 100 to 2,000 m/z antibiotics and sulfonamides increases Choung 2007, 2010;Li and Zhang 2010;Zhang et al 2011), the degradation of sulfadoxine by Pseudomonas sp. DX7 may be potentially exploited to eliminate sulfadoxine contamination from the environment.…”

Section: Degradation Of Sulfadoxine By Pseudomonas Sp Dx7mentioning

confidence: 99%

“…and Acinetobacter sp. have been shown to degrade sulfapyridine and sulfathiazole (Zhang et al 2011). Until now, no specific bacterial strain had been isolated with the capacity to degrade sulfadoxine.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of Pseudomonas sp. DX7 capable of degrading sulfadoxine

Zhang

Niu

et al. 2011

Biodegradation

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Given that the intensive application of sulfonamides in aquaculture, animal husbandry and malaria treatment has lead to an increase in sulfonamide discharge into the environment, there is an increasing need to find a way to remediate sulfonamide-contaminated sites. The bacterial strain DX7 was isolated from a marine environment and is capable of degrading sulfadoxine. DX7 was identified as a Pseudomonas sp.based on 16S rRNA gene sequencing. Approximately 30% of sulfadoxine was degraded after Pseudomonas sp. DX7 was inoculated into mineral salt plus tryptone media containing 10 mg l -1 sulfadoxine for 2 days. The degradation efficiency under different environmental conditions was characterized using HPLC. The optimal temperature and pH for sulfadoxine biodegradation were around 30°C and 6.0, respectively. The optimal concentrations of sulfadoxine and tryptone for sulfadoxine biodegradation were determined to be approximately 30 mg l -1 and between 2.0 and 8.0 g l -1, respectively. Cytotoxicity analysis indicated that the metabolites of sulfadoxine generated by Pseudomonas sp. DX7 showed significantly reduced cytotoxicity to Hela cells. These results suggest that Pseudomonas sp. DX7 is a new bacterial resource for degrading sulfadoxine and indicate the potential of the isolated strain in the bioremediation of sulfadoxinecontaminated environments.

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Isolation and characterization of sulfonamide-degrading bacteria Escherichia sp. HS21 and Acinetobacter sp. HS51

Cited by 53 publications

References 29 publications

Degradation of Sulfadiazine by Microbacterium lacus Strain SDZm4, Isolated from Lysimeters Previously Manured with Slurry from Sulfadiazine-Medicated Pigs

Degradation of Sulfadiazine by Microbacterium lacus Strain SDZm4, Isolated from Lysimeters Previously Manured with Slurry from Sulfadiazine-Medicated Pigs

Isolation and characterization of Pseudomonas sp. strain capable of degrading diethylstilbestrol

Isolation and characterization of Pseudomonas sp. DX7 capable of degrading sulfadoxine

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